NFS1 (Myc-DDK-tagged)-Human NFS1 nitrogen fixation 1 homolog (S. cerevisiae) (NFS1), nuclear gene encoding mitochondrial protein, transcript variant 1
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
NFS1 (Myc-DDK-tagged)-Human NFS1 nitrogen fixation 1 homolog (S. cerevisiae) (NFS1), nuclear gene encoding mitochondrial protein, transcript variant 1
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
NFS1 (Myc-DDK-tagged)-Human NFS1 nitrogen fixation 1 homolog (S. cerevisiae) (NFS1), transcript variant 3
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
NFS1 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
Purity | HPLC purified |
Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
NFS1 - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated.
Format | 2 gRNA vectors, 1 linear donor |
Donor DNA | EF1a-GFP-P2A-Puro |
Transient overexpression lysate of NFS1 nitrogen fixation 1 homolog (S. cerevisiae) (NFS1), nuclear gene encoding mitochondrial protein
Tag | C-Myc/DDK |
Expression Host | HEK293T |
NFS1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
Format | Retroviral plasmids |
Vector | pRS |
NFS1 HEK293T cell transient overexpression lysate (as WB positive control)
Tag | C-Myc/DDK |
Expression Host | HEK293T |
NFS1 CRISPRa kit - CRISPR gene activation of human NFS1 cysteine desulfurase
Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Transient overexpression lysate of NFS1 nitrogen fixation 1 homolog (S. cerevisiae) (NFS1), transcript variant 3
Tag | C-Myc/DDK |
Expression Host | HEK293T |
qPCR primer pairs and template standards against Homo sapiens gene NFS1
Application | Plasmid of exact quantity for transcript copy number calculation |
qSTAR qPCR primer pairs against Homo sapiens gene NFS1
Transient overexpression of NFS1, transcript variant 1, in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack
Transient overexpression of NFS1, transcript variant 3, in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack
NFS1 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
Format | Lentiviral plasmids |
Vector | pGFP-C-shLenti |
NFS1 (tGFP-tagged) - Human NFS1 nitrogen fixation 1 homolog (S. cerevisiae) (NFS1), nuclear gene encoding mitochondrial protein, transcript variant 1
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Vector | pCMV6-AC-GFP |