MGMT (Myc-DDK-tagged)-Human O-6-methylguanine-DNA methyltransferase (MGMT)
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
Products
Recombinant protein of human O-6-methylguanine-DNA methyltransferase (MGMT), 20 µg
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
MGMT (Myc-DDK-tagged)-Human O-6-methylguanine-DNA methyltransferase (MGMT)
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
- TrueORF®
MGMT - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pGFP-V-RS |
MGMT (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
3' UTR clone of O-6-methylguanine-DNA methyltransferase (MGMT) for miRNA target validation
| Vector | pMirTarget |
| Species | Human |
| Transfection Reporter | RFP |
| Assay Reporter | Luciferase |
MGMT - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated.
| Format | 2 gRNA vectors, 1 linear donor |
| Donor DNA | EF1a-GFP-P2A-Puro |
Recombinant protein of human O-6-methylguanine-DNA methyltransferase (MGMT), 1 mg
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
Recombinant protein of human O-6-methylguanine-DNA methyltransferase (MGMT), 100 µg
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
Recombinant protein of human O-6-methylguanine-DNA methyltransferase (MGMT), full length, with N-terminal HIS tag, expressed in E.Coli, 50ug
| Tag | N-His |
| Expression Host | E. coli |
MGMT - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
Transient overexpression lysate of O-6-methylguanine-DNA methyltransferase (MGMT)
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
MGMT - human gene knockout kit via CRISPR, HDR mediated
| Format | 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control |
| Donor DNA | GFP-puro |
MGMT CRISPRa kit - CRISPR gene activation of human O-6-methylguanine-DNA methyltransferase
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
MGMT - human gene knockout kit via CRISPR, HDR mediated
| Format | 2 gRNA vectors, 1 Luciferase-Puro donor, 1 scramble control |
| Donor DNA | Luciferase-Puro |
