GUCA2A CRISPRa kit - CRISPR gene activation of human guanylate cyclase activator 2A
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Products
qSTAR qPCR primer pairs against Homo sapiens gene GUCA2A
Guanylin human recombinant protein, 0.1 mg
| Expression Host | E. coli |
GUCA2A (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
活动过滤器
优化搜索结果
Expression Hosts
Families
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Adermann K. Role of the prosequence of guanylin. Protein Sci. 1999 Sep; 8(9): 1850-9.
4. Lauber T (1) -
Cohen MB. Proguanylin secretion and the role of negative-feedback inhibition in a villous epithelial cell line. Am J Physiol Gastrointest Liver Physiol. 2002 Sep; 283(3): G695-702.
3. Schulz A (1) - Forssmann WG (1)
- Hawkins JA (1)
- Hidaka Y (1)
- Marx UC (1)
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Marx UC. Native and recombinant proguanylin feature identical biophysical properties and are monomeric in solution. Biochemistry. 2002 Dec 10; 41(49): 14602-12.
2. Rudolph JA (1) - Marx UC. Solution structure of human proguanylin: the role of a hormone prosequence. J Biol Chem. 2003 Jun 27; 278(26): 24118-24. Epub 2003 Apr 21. (1)
- Neudecker P (1)
- Nourse A (1)
- Rosch P (1)
- Schulz A (1)
- Shimonishi Y (1)
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Western Blot.
Protocol: 1. Lauber T (1)
