DVL1 (Myc-DDK-tagged)-Human dishevelled, dsh homolog 1 (Drosophila) (DVL1)
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
Products
DVL1 (Myc-DDK-tagged)-Human dishevelled, dsh homolog 1 (Drosophila) (DVL1), transcript variant 3
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
DVL1 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pGFP-V-RS |
DVL1 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
DVL1 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
DVL1 - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated.
| Format | 2 gRNA vectors, 1 linear donor |
| Donor DNA | EF1a-GFP-P2A-Puro |
Purified recombinant protein of Human dishevelled, dsh homolog 1 (Drosophila) (DVL1), Met1-Tyr279, with N-terminal His tag, expressed in E.coli, 50ug
| Tag | N-His |
| Expression Host | E. coli |
Transient overexpression lysate of dishevelled, dsh homolog 1 (Drosophila) (DVL1)
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
DVL1 HEK293T cell transient overexpression lysate (as WB positive control)
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
DVL1 CRISPRa kit - CRISPR gene activation of human dishevelled segment polarity protein 1
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Transient overexpression lysate of dishevelled, dsh homolog 1 (Drosophila) (DVL1), transcript variant 3
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
qPCR primer pairs and template standards against Homo sapiens gene DVL1
| Application | Plasmid of exact quantity for transcript copy number calculation |
qSTAR qPCR primer pairs against Homo sapiens gene DVL1
Transient overexpression of DVL1, transcript variant 3, in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack
Transient overexpression of DVL1 in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack
