Products
GFAP (417-430) goat polyclonal antibody, Aff - Purified
| Applications | ELISA, IHC, WB |
| Reactivities | Human, Rat, Canine, Mouse, Bovine, Bat, Equine, Monkey, Rabbit |
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- ES Cell Differentiation/IPS (108)
- WB (4)
- 10 mM Tris-HCl (2)
- 2 mM dithiothreitol (2)
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Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to Filaments is performed by dissolving in 6 M urea buffer (see above) at concentrations of approx. 0.5 mg/ml. Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl (2) - e.g. Dulbeccos PBS). (2)
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pH 7.4).
For immunization purposes (2) - the solution can be further dialyzed against PBS (phosphate buffered saline (2)
