Human IgE mouse monoclonal antibody, clone I27/mAb27
| Applications | FACS surface, Western Blot, Basophil degranulation, Study of IgE binding to CD23 (Fc epsilon R2). Usage recommendation: *This monoclonal antibody may be used between 1-10 μg/ml. *Optimal dilution should be determined by each laboratory for each application. *Coupled antibody: to maintain RT before use. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgA (alpha chain) goat polyclonal antibody, Biotin
| Applications | Immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates: ELISA: 1/60,000-1/600,000 Western Blot: 1/2,000-1/10,000 Immunohistochemistry: 1/1,000-1/5,000 |
| Reactivities | Human |
| Conjugation | Biotin |
Human IgA (alpha chain) goat polyclonal antibody, Aff - Purified
| Applications | Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. Recommended Dilutions: ELISA: 1/80,000. Wester Blot: 1/2,000-1/10,000. Immunohistochemistry: 1/1,000-1/5,000. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Mouse IgG (Fc specific) goat polyclonal antibody, Ig Fraction
| Applications | ELISA: 1/20000-1/100000. Western Blot: 1/2000-1/10000. Immunohistochemistry: 1/1000-1/5,000. |
| Reactivities | Mouse |
| Conjugation | Unconjugated |
Chicken IgG / Chicken IgY mouse monoclonal antibody, clone 4G12, Purified
| Applications | Western Blot: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. ELISA: 0.1 µg/ml. Immunoprecipitation: 1 - 10 µg. |
| Reactivities | Chicken |
| Conjugation | Unconjugated |
Chicken IgM (Fc specific) goat polyclonal antibody, FITC
| Applications | Can be used in Immunocytochemical and Immunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of chicken origin known to be of the IgM isotype in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended working dilutions: 1/10 - 1/40, depending on the method used. |
| Reactivities | Chicken |
| Conjugation | FITC |
Mouse IgG goat polyclonal antibody, AP, Aff - Purified
| Applications | ELISA: 1/10000. Western Blot: 1/5000-1/10000, Immunohistochemistry. |
| Reactivities | Mouse |
| Conjugation | AP |
Porcine IgG (H+L chain) rabbit polyclonal antibody, TRITC
| Applications | ELISA. Immunocytochemistry. Immunofluorescence. Immunohistochemistry Frozen Sections. Can be used to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates. If two Nordic immunoconjugates with different labels but originating from the same host animal species are used for direct two-colour staining, they can safely be mixed and used in a single step, without the danger of interaction between the two reagents. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: 1/20-1/80. |
| Reactivities | Porcine |
| Conjugation | TRITC |
Porcine IgG (H+L chain) rabbit polyclonal antibody, FITC
| Applications | ELISA. Immunocytochemistry. Immunofluorescence. Immunohistochemistry Frozen Sections. Can be used to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of swine origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: 1/20-1/80. |
| Reactivities | Porcine |
| Conjugation | FITC |
Mouse IgG goat polyclonal antibody, HRP, Aff - Purified
| Applications | ELISA: 1/10000. Western Blot: 1/5000-1/10000, Immunohistochemistry. |
| Reactivities | Mouse |
| Conjugation | HRP |
Sheep IgM (H+L chain) rabbit polyclonal antibody, Azide Free
| Applications | ELISA. Dot blot. Immunoblotting. Indirect Immunofluorescence. Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Recommended Working Dilutions: Histochemistry Use: 1/50-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000. Precipitin titre: 1/64 when tested against pooled normal sheep serum in agar-block immunodiffusion titration. |
| Reactivities | Sheep |
| Conjugation | Unconjugated |
Sheep IgM (Fc specific) rabbit polyclonal antibody, FITC
| Applications | ELISA. Dot blot. Immunoblotting. Immunocytochemistry. Immunohistochemistry Frozen Sections. Can be used in immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of sheep origin known to be of the IgM isotype in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: 1/20-1/80. |
| Reactivities | Sheep |
| Conjugation | FITC |
Sheep IgM (Fc specific) rabbit polyclonal antibody, Azide Free
| Applications | ELISA. Dot blot. Immunoblotting. Indirect Immunofluorescence. Can be used as unlabelled primary or secondary reagent for indirect detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to prepare conjugates of the user’s own choice; to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in nonisotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used. Recommended Working Dilutions: Histochemistry Use: 1/50-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000. Precipitin titre: 1/64 when tested against pooled normal sheep serum in agar-block immunodiffusion titration. |
| Reactivities | Sheep |
| Conjugation | Unconjugated |
Porcine IgG (H+L chain) rabbit polyclonal antibody, Azide Free
| Applications | ELISA. Dot blot. Immunoblotting. Indirect Immunofluorescence. Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Recommended Working Dilutions: Histochemistry Use: 1/50-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000. Precipitin titre: 1/64 when tested against pooled normal Swine serum in agar-block immunodiffusion titration. |
| Reactivities | Porcine |
| Conjugation | Unconjugated |
Sheep IgM (H+L chain) rabbit polyclonal antibody, FITC
| Applications | ELISA. Dot blot. Immunoblotting. Immunocytochemistry. Immunohistochemistry Frozen Sections. Can be used to identify IgM at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases, where IgM and IgG antibodies can be expected. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: 1/20-1/80. |
| Reactivities | Sheep |
| Conjugation | FITC |
