Lenti ORF clone of Vim (Myc-DDK-tagged) - Mouse vimentin (Vim)
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
- LentiORF®
Products
VIM (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
SR322185 is the updated version of SR305078.
Vim (untagged) - Mouse vimentin (Vim), (10ug)
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
Vim (untagged ORF) - Rat vimentin (Vim), (10 ug)
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
Purified recombinant protein of Mouse vimentin (Vim), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
| Tag | C-MYC/DDK |
| Expression Host | HEK293T |
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Species
Reactivities
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Antibody Hosts
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Lentivirus
Vectors
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Knockout (KO) Validation
Conjugates
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- e.g. Dulbecco's PBS). (4)
- 10 mM sodium phosphate (2)
- 10mM Tris-HCl (2)
- 2 mM dithiothreitol (2)
- 2mM dithiothreitol (2)
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Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above) (2) -
Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after vimentin is dissolved in 6 M urea buffer (see above) (2) - pH 7.4). For immunization purposes (2)
-
pH 7.4).
For immunization purposes (2) - protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl (2)
- protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl (2)
- the solution can be further dialyzed against PBS (Phosphate Buffered Saline (2)
- the solution can be further dialyzed against PBS (phosphate buffered saline (2)
