Apmap - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated.
| Format | 2 gRNA vectors, 1 linear donor |
| Donor DNA | EF1a-GFP-P2A-Puro |
Products
C20orf3 mouse monoclonal antibody, clone OTI1D5 (formerly 1D5), Biotinylated
| Applications | WB |
| Reactivities | Human, Mouse, Rat |
C20orf3 mouse monoclonal antibody, clone OTI1D5 (formerly 1D5), HRP conjugated
| Applications | WB |
| Reactivities | Human, Mouse, Rat |
Transient overexpression lysate of chromosome 20 open reading frame 3 (C20orf3)
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
APMAP CRISPRa kit - CRISPR gene activation of human adipocyte plasma membrane associated protein
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Apmap CRISPRa kit - CRISPR gene activation of mouse adipocyte plasma membrane associated protein
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
qPCR primer pairs and template standards against Mus musculus gene 2310001A20Rik
| Application | Plasmid of exact quantity for transcript copy number calculation |
Apmap (Rat) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
qSTAR qPCR primer pairs against Homo sapiens gene APMAP
qSTAR qPCR primer pairs against Mus musculus gene Apmap
Transient overexpression of APMAP in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack
APMAP - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
Apmap - Rat, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
Apmap - Rat, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
APMAP - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
