UBE2S mouse monoclonal antibody, clone OTI4E4 (formerly 4E4)
| Applications | IF, WB |
| Reactivities | Human, Mouse, Rat |
Products
UBE2S CRISPRa kit - CRISPR gene activation of human ubiquitin conjugating enzyme E2 S
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
UBE2S MS Standard C13 and N15-labeled recombinant protein (NP_055316)
| Tag | C-Myc/DDK |
| Expression Host | HEK293 |
3' UTR clone of ubiquitin-conjugating enzyme E2S (UBE2S) for miRNA target validation
| Vector | pMirTarget |
| Species | Human |
| Transfection Reporter | RFP |
| Assay Reporter | Luciferase |
qPCR primer pairs and template standards against Homo sapiens gene UBE2S
| Application | Plasmid of exact quantity for transcript copy number calculation |
qPCR primer pairs and template standards against Homo sapiens gene UBE2S
| Application | Plasmid of exact quantity for transcript copy number calculation |
qSTAR qPCR primer pairs against Mus musculus gene Ube2s
qPCR primer pairs and template standards against Homo sapiens gene UBE2S
| Application | Plasmid of exact quantity for transcript copy number calculation |
Purified recombinant protein of Mouse ubiquitin-conjugating enzyme E2S (Ube2s), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
| Tag | C-MYC/DDK |
| Expression Host | HEK293T |
Transient overexpression of UBE2S in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack
Ube2s - Rat, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
Ube2s (Rat) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
qSTAR qPCR primer pairs against Homo sapiens gene UBE2S
Ube2s - Rat, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
Ube2s - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
