Lenti ORF clone of Krt14 (Myc-DDK-tagged ORF) - Rat keratin 14 (Krt14), (10 ug)
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
- LentiORF®
Products
Lenti ORF clone of Krt14 (Myc-DDK-tagged) - Mouse keratin 14 (Krt14)
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
- LentiORF®
Lenti ORF clone of Krt14 (mGFP-tagged ORF) - Rat keratin 14 (Krt14), (10 ug)
| Type | Rat Tagged ORF Clone |
| Tag | mGFP |
| Vector | pLenti-C-mGFP-P2A-Puro |
- LentiORF®
Krt14 (untagged ORF) - Rat keratin 14 (Krt14), (10 ug)
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
Krt14 (untagged) - Mouse keratin 14 (Krt14), (10ug)
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Vector | PCMV6-Kan/Neo |
Krt14 (untagged) - Mouse keratin 14 (cDNA clone MGC:7044 IMAGE:3156333), (10ug)
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Vector | PCMV6-Kan/Neo |
KRT14 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
Krt14 (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
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- 10 mM Tris-HCl (2)
- 2 mM dithiothreitol (2)
-
Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml (2) - both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl (2)
- e.g. Dulbecco's PBS). (2)
-
pH 7.4).
For immunization purposes (2) - the solution can be further dialyzed against PBS (phosphate buffered saline (2)
