Kmt5a - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated.
| Format | 2 gRNA vectors, 1 linear donor |
| Donor DNA | EF1a-GFP-P2A-Puro |
Products
KMT5A (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
SETD8 mouse monoclonal antibody, clone OTI2F5 (formerly 2F5)
| Applications | IHC, WB |
| Reactivities | Human |
Transient overexpression lysate of SET domain containing (lysine methyltransferase) 8 (SETD8)
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
SETD8 mouse monoclonal antibody, clone OTI5E5 (formerly 5E5)
| Applications | WB |
| Reactivities | Human |
SETD8 mouse monoclonal antibody, clone OTI2B3 (formerly 2B3)
| Applications | WB |
| Reactivities | Human |
SETD8 mouse monoclonal antibody, clone OTI5C7 (formerly 5C7)
| Applications | WB |
| Reactivities | Human |
Kmt5a CRISPRa kit - CRISPR gene activation of mouse lysine methyltransferase 5A
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
KMT5A CRISPRa kit - CRISPR gene activation of human lysine methyltransferase 5A
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
3' UTR clone of SET domain containing (lysine methyltransferase) 8 (SETD8) for miRNA target validation
| Vector | pMirTarget |
| Species | Human |
| Transfection Reporter | RFP |
| Assay Reporter | Luciferase |
qPCR primer pairs and template standards against Homo sapiens gene SETD8
| Application | Plasmid of exact quantity for transcript copy number calculation |
qSTAR qPCR primer pairs against Mus musculus gene Kmt5a
qSTAR qPCR primer pairs against Homo sapiens gene KMT5A
Transient overexpression of KMT5A in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack
KMT5A - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
