Rabbit IgG (H+L chain, adsorbed) goat polyclonal antibody, Aff - Purified
| Applications | Suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user. ELISA: 1/15,000-1/30,000 Western blot: 1/500-1/2,000 Immunohistochemistry: 1/500-1/2,000. |
| Reactivities | Rabbit |
| Conjugation | Unconjugated |
Canine IgE goat polyclonal antibody, HRP
| Applications | ELISA: 1/10,000-1/100,000. Western Blot: 1/1000-1/10,000. Immunohistochemistry on Frozen Sections: 1/200-1/500. |
| Reactivities | Canine |
| Conjugation | HRP |
Canine IgG1 goat polyclonal antibody, Purified
| Applications | ELISA: 1:100 - 1:10,000. Western Blot: 1:1000 - 1:10,000. Immunohistochemistry on frozen sections: 1:200 - 1:2000. |
| Reactivities | Canine |
| Conjugation | Unconjugated |
Human IgG3 (Hinge Region) mouse monoclonal antibody, clone HP6050, AP
| Applications | ELISA: 1/500-1/1,000. Western blotting. Dot- and Slot-Immunoblotting Immunohistochemistry on Frozen Sections. |
| Reactivities | Human |
| Conjugation | AP |
Human IgM mouse monoclonal antibody, clone M15/8, Purified
| Applications | Flow Cytometry: 20 µg/ml. Immunohistochemistry on frozen sections: 20 µg/ml. ELISA: 5 µg/ml as coating Antibody. Immunoprecipitation. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgG (Fc specific) goat polyclonal antibody, Aff - Purified
| Applications | ELISA: 1-5 µg/ml. Immunohistochemistry on Frozen Sections. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Canine IgE mouse monoclonal antibody, clone E6-2A1, Purified
| Applications | Western Blot. ELISA: The antibody may be used as a Capture antibody in ELISA in combination with SM1499B as detecting antibody. |
| Reactivities | Canine |
| Conjugation | Unconjugated |
Rat IgG2ab (subclass specific) goat polyclonal antibody, TRITC
| Applications | Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgG2 at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to identify and measure IgG2 in rat serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions 1/20 - 1/80, depending on the method used. |
| Reactivities | Rat |
| Conjugation | TRITC |
Rat IgG2ab (subclass specific) goat polyclonal antibody, FITC
| Applications | Can be used to identify and measure IgG2, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of rat origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions: 1/20 - 1/80. |
| Reactivities | Rat |
| Conjugation | FITC |
Rat IgG2ab (subclass specific) goat polyclonal antibody, Azide Free
| Applications | As unlabelled primary or secondary reagent for indirect detection of IgG2a at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to prepare conjugates of the user’s own choice; to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used. Recommended working dilutions: Histochemistry: 1/100 - 1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500 - 1/5000. |
| Reactivities | Rat |
| Conjugation | Unconjugated |
Rat IgG2ab (subclass specific) goat polyclonal antibody, Biotin
| Applications | Can be used: In immunocytochemical and Immunohistochemical staining for the detection of IgG2 at the cellular and subcellular level of appropriately treated cell and tissue substrates. To demonstrate circulating IgG2 antibodies in serodiagnostic microbiology and autoimmune diseases. To identify a specific antigen using a reference antibody of Rat origin known to be of the IgG2 isotype in the middle layer of the indirect test procedure. In non-isotopic assay methodology (e.g. ELISA) to measure IgG2 in rat serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user's choice has to be used. This immunoconjugate is not pre-diluted. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemical and Cytochemical Use: 1/100-1/250. ELISA and comparable non-precipitating antibody-binding assays between: 1/2.000-1/10,000. |
| Reactivities | Rat |
| Conjugation | Biotin |
Mouse IgG3 (subclass specific) goat polyclonal antibody, TRITC
| Applications | Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgG3 at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to identify and measure IgG3 in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions 1/10 - 1/40, depending on the method used. |
| Reactivities | Mouse |
| Conjugation | TRITC |
Mouse IgG3 (subclass specific) goat polyclonal antibody, Azide Free
| Applications | Can be used as unlabelled primary or secondary reagent for indirect detection of IgG3 at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to prepare conjugates of the user’s own choice; to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used. Recommended Working Dilutions: Histochemistry: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5.000. Performance testing: Titre, specificity and reactivity of the subclass specific cross-adsorbed IgG fraction is further evaluated in a number of highly sensitive non-precipitating antibody-binding assay systems. These performance tests include direct single and double identification of cIg in mouse cell and tissue substrates and their evaluation as second antibody in the analysis of human cells and tissues after reacting with a primary monoclonal mouse antibody to a human antigen. The quantitative specific recognition ability is verified in double staining procedures together with reference reagents of known specificity and reactivity. |
| Reactivities | Mouse |
| Conjugation | Unconjugated |
Rabbit IgA+IgG+IgM (H+L chain) goat polyclonal antibody, Azide Free
| Applications | The cytochemical grade allows the use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates; in radioimmunoassay; for the production of immunoconjugates with a selected marker; to prepare immunoaffinity adsorbents by coupling to an artificial carrier; in non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent; in Western blotting. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used. Recommended Working Dilutions: Histochemical and Cytochemical Use: 1/100-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5000. |
| Reactivities | Rabbit |
| Conjugation | Unconjugated |
Rabbit IgA (Fc specific) goat polyclonal antibody, Azide Free
| Applications | As unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Recommended Working Dilutions: Histochemistry: 1/100-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/2500. Antibody titre: Precipitin titre not less than 1/32 when tested against pooled normal rabbit serum in agar-block immunodiffusion titration. |
| Reactivities | Rabbit |
| Conjugation | Unconjugated |
