Setdb1 - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated.
| Format | 2 gRNA vectors, 1 linear donor |
| Donor DNA | EF1a-GFP-P2A-Puro |
Products
Setdb1 (Myc-DDK-tagged) - Mouse SET domain, bifurcated 1 (Setdb1), transcript variant 2
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
- TrueORF®
Setdb1 (Myc-DDK-tagged) - Mouse SET domain, bifurcated 1 (Setdb1), transcript variant 3
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
- TrueORF®
SETDB1 (Myc-DDK tagged) - Homo sapiens SET domain, bifurcated 1 (SETDB1), transcript variant 3
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
- TrueORF®
Purified recombinant protein of Homo sapiens SET domain, bifurcated 1 (SETDB1), transcript variant 2, 1 mg
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
Purified recombinant protein of Homo sapiens SET domain, bifurcated 1 (SETDB1), transcript variant 2, 100 µg
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
Transient overexpression lysate of SET domain, bifurcated 1 (SETDB1), transcript variant 2
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
Transient overexpression lysate of SET domain, bifurcated 1 (SETDB1), transcript variant 1
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
Lenti ORF clone of Human SET domain, bifurcated 1 (SETDB1), transcript variant 1, mGFP tagged
| Type | Human Tagged ORF Clone |
| Tag | mGFP |
| Vector | pLenti-C-mGFP |
- LentiORF®
SETDB1 CRISPRa kit - CRISPR gene activation of human SET domain bifurcated histone lysine methyltransferase 1
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Setdb1 CRISPRa kit - CRISPR gene activation of mouse SET domain, bifurcated 1
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
qPCR primer pairs and template standards against Homo sapiens gene SETDB1
| Application | Plasmid of exact quantity for transcript copy number calculation |
qSTAR qPCR primer pairs against Mus musculus gene Setdb1
qSTAR qPCR primer pairs against Homo sapiens gene SETDB1
Setdb1 (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
