HADHA CRISPRa kit - CRISPR gene activation of human hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit alpha
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Products
HADHA - human gene knockout kit via CRISPR, HDR mediated
| Format | 2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control |
| Donor DNA | RFP-BSD |
Hadha CRISPRa kit - CRISPR gene activation of mouse hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
HADHA - human gene knockout kit via CRISPR, HDR mediated
| Format | 2 gRNA vectors, 1 mBFP-Neo donor, 1 scramble control |
| Donor DNA | mBFP-Neo |
HADHA - human gene knockout kit via CRISPR, HDR mediated
| Format | 2 gRNA vectors, 1 Luciferase-Puro donor, 1 scramble control |
| Donor DNA | Luciferase-Puro |
qSTAR qPCR primer pairs against Homo sapiens gene HADHA
HADHA - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRFP-C-RS |
qPCR primer pairs and template standards against Mus musculus gene Hadha
| Application | Plasmid of exact quantity for transcript copy number calculation |
qPCR primer pairs and template standards against Homo sapiens gene HADHA
| Application | Plasmid of exact quantity for transcript copy number calculation |
Hadha (Rat) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
Hadha (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
qSTAR qPCR primer pairs against Mus musculus gene Hadha
Transient overexpression of HADHA in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack
Hadha - Rat, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
Hadha - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
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Knockout (KO) Validation
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- Biosynthesis of unsaturated fatty acids (18)
- Butanoate metabolism (18)
- Fatty acid elongation in mitochondria (18)
- Fatty acid metabolism (18)
- Limonene and pinene degradation (18)
- Lysine degradation (18)
- Metabolic pathways (18)
- Propanoate metabolism (18)
- Tryptophan metabolism (18)
- Valine (18)
- beta-Alanine metabolism (18)
- leucine and isoleucine degradation (18)
