Gpr83 - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated.
| Format | 2 gRNA vectors, 1 linear donor |
| Donor DNA | EF1a-GFP-P2A-Puro |
Products
Transient overexpression lysate of G protein-coupled receptor 83 (GPR83)
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
GPR83 mouse monoclonal antibody,clone OTI13B6
| Applications | WB |
| Reactivities | Mouse, Rat |
GPR83 mouse monoclonal antibody,clone OTI6C6
| Applications | WB |
| Reactivities | Mouse, Rat |
GPR83 mouse monoclonal antibody,clone OTI3E8
| Applications | WB |
| Reactivities | Rat, Mouse |
GPR83 CRISPRa kit - CRISPR gene activation of human G protein-coupled receptor 83
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Gpr83 CRISPRa kit - CRISPR gene activation of mouse G protein-coupled receptor 83
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
qPCR primer pairs and template standards against Homo sapiens gene GPR83
| Application | Plasmid of exact quantity for transcript copy number calculation |
Transient overexpression of GPR83 in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack
Gpr83 - Rat, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
GPR83 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
Gpr83 - Rat, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
GPR83 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
Gpr83 (Rat) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
qSTAR qPCR primer pairs against Homo sapiens gene GPR83
