Ago1 - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated.
| Format | 2 gRNA vectors, 1 linear donor |
| Donor DNA | EF1a-GFP-P2A-Puro |
Products
Purified recombinant protein of Human eukaryotic translation initiation factor 2C, 1 (EIF2C1), Gln226-Cys490, with N-terminal HIS tag, expressed in E. coli, 50ug
| Tag | N-His |
| Expression Host | E. coli |
Purified recombinant protein of Human eukaryotic translation initiation factor 2C, 1 (EIF2C1), full length, with N-terminal GST and C-terminal His tag, expressed in E.coli, 50ug
| Tag | N-GST and C-HIS |
| Expression Host | E. coli |
EIF2C1 mouse monoclonal antibody,clone OTI2E8
| Applications | WB |
| Reactivities | Human, Mouse, Rat |
Transient overexpression lysate of eukaryotic translation initiation factor 2C, 1 (EIF2C1)
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
EIF2C1 mouse monoclonal antibody,clone OTI3F5
| Applications | WB |
| Reactivities | Human, Mouse, Rat |
AGO1 CRISPRa kit - CRISPR gene activation of human argonaute RISC component 1
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Ago1 CRISPRa kit - CRISPR gene activation of mouse argonaute RISC catalytic subunit 1
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
qPCR primer pairs and template standards against Homo sapiens gene EIF2C1
| Application | Plasmid of exact quantity for transcript copy number calculation |
qSTAR qPCR primer pairs against Homo sapiens gene AGO1
Ago1 (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
qSTAR qPCR primer pairs against Mus musculus gene Ago1
Transient overexpression of AGO1 in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack
AGO1 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
Ago1 - Rat, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
