GFAP mouse monoclonal antibody, clone OTI1H7
| Applications | ELISA |
| Reactivities | Human |
Products
GFAP mouse monoclonal antibody, clone OTI4D12
| Applications | ELISA |
| Reactivities | Human |
GFAP mouse monoclonal antibody, clone OTI4B5
| Applications | ELISA |
| Reactivities | Human |
GFAP mouse monoclonal antibody, clone OTI1F4
| Applications | ELISA |
| Reactivities | Human |
GFAP mouse monoclonal antibody, clone OTI3E8
| Applications | ELISA |
| Reactivities | Human |
GFAP mouse monoclonal antibody, clone OTI9H6
| Applications | ELISA |
| Reactivities | Human |
GFAP Rabbit polyclonal Antibody
| Applications | ELISA, ICC/IF, IHC, WB |
| Reactivities | Human, Mouse, Rat |
| Modifications | Unmodified |
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qPCR primer pairs and template standards against Homo sapiens gene GFAP
| Application | Plasmid of exact quantity for transcript copy number calculation |
GFAP - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
Gfap (Rat) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
Transient overexpression of GFAP, transcript variant 2, in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack
Gfap - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
Gfap - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
Gfap - Rat, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
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- ES Cell Differentiation/IPS (201)
- WB (4)
- 10 mM Tris-HCl (2)
- 2 mM dithiothreitol (2)
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Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to Filaments is performed by dissolving in 6 M urea buffer (see above) at concentrations of approx. 0.5 mg/ml. Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl (2) - e.g. Dulbeccos PBS). (2)
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pH 7.4).
For immunization purposes (2) - the solution can be further dialyzed against PBS (phosphate buffered saline (2)
