Products

Proteins

Over-expression Lysates

Human

Mouse

SARS-CoV-2

BDNF

CONSTITUTIVE ANDROSTANE RECEPTOR

CD59

PDE9A

ALPHA 1 ANTITRYPSIN

TGIF

BIN1

CACNB2

CAMK2B

CHRM2

HNRNP F

NAT1

NCALD

NDRG2

PPP2R2B

RIBONUCLEASE INHIBITOR

UBE2D3

IRF5

14-3-3 ZETA

ABLIM2

APC11

APRATAXIN

BANP

CD10

GABA A RECEPTOR ALPHA 1

HMGA1

MBNL1

NRG1

P2X2

RNASE9

TCF7L2

TTC23

ZFYVE27

APOL3

MTH1

ALKALINE PHOSPHATASE

ARCHAEMETZINCIN 2

AURORA A

CABYR

CASPASE-7

CATHEPSIN B

CD1E

CLAUDIN 14

DISC1

DYSTROBREVIN ALPHA

DYSTROPHIN

EYA1

FHL1

ICAM2

IL32

MU OPIOID RECEPTOR

MYADM

MYELIN BASIC PROTEIN

NEK6

OPA1

OPTINEURIN

OSBPL9

PAX2

PCBP2

PQBP1

PRKAR1B

RAD17

RBFOX1

SIGLEC6

SSH3BP1

STEROL CARRIER PROTEIN 2

TPM1

TSPAN4

ZBTB20

ZFP200

MYOSIN

TAU

ACRV1

ARSA

ATAXIN 3

ATF3

B3GALT5

BCAT1

CAMKK2

CARBONIC ANHYDRASE I

CCM2

CD32B

CERKL

CHID1

CLDND1

CLEAVAGE STIMULATION FACTOR

CREM

CRISP2

CUG BP1

DGKA

DGLUCY

DHRS9

DOUBLECORTIN

EEF1D

ER81

FAM111A

FGFR1

FGFR4

FGL1

FOLATE BINDING PROTEIN

WB

HEK293T

C-Myc/DDK

C-MYC/DDK

Druggable Genome

Transmembrane

WB

Transcription Factors

Secreted Protein

Protein Kinase

ES Cell Differentiation/IPS

Protease

Stem cell - Pluripotency

GPCR

Phosphatase

Adult stem cells

Ion Channels: Other

Embryonic stem cells

Nuclear Hormone Receptor

Cancer stem cells

Ion Channels: Potassium

Induced pluripotent stem cells

P450

Stem cell relevant signaling - JAK/STAT signaling pathway

Ion Channels: Cys-loop Receptors

Stem cell relevant signaling - TGFb/BMP signaling pathway

Stem cell relevant signaling - DSL/Notch pathway

Ion Channels: Transient receptor potential

Stem cell relevant signaling - Wnt Signaling pathway

Ion Channels: Glutamate Receptors

Ion Channels: Calcium

Ion Channels: Sodium

Ion Channels: ATP Receptors

Ion Channels: Cyclic nucleotide gated

ELISA

SDS-PAGE

Bioactivity

the solution can be further dialyzed against PBS (phosphate buffered saline

10 mM Tris-HCl

2 mM dithiothreitol

Enzyme Activity

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g. Dulbecco's PBS).

ELISA.
Western Blot.

Flow Cyt

pH 7.4). For immunization purposes

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.

pH 7.4).
For immunization purposes

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

e.g. Dulbeccos PBS).

e.g. Dulbeccos PBS).
See References 2 and 3 for more details.

pH 7.4).
For immunization purposes

pH 7.4).
For Immunization purposes

Biological activity/ Working concentration: Optimum concentration for human umbilical vein endothelial cells (HUVEC) range from 50-200 μg/ml

ELISA.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoblotting.
Receptor-binding studies.

Western Blot.

0.1% (w/v) insulin

000-1/5

10 mM sodium phosphate

10mM Tris-HCl

1mM DTT

2 mM dithiothreitol

2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes

2mM dithiothreitol

63mM sodium phosphate

Biological activity/ Working concentration: The working concentration of ECGF for HUVEC is in the range of 25µg/ml to 100µg/ml. When adding Heparin (2.5mg per mg ECGF) an ECGF concentration of 12µg/ml (30µg/ml Heparin) is optimal. In this case 6mg ECGF are sufficient for 500 ml medium.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above)

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to Filaments is performed by dissolving in 6 M urea buffer (see above) at concentrations of approx. 0.5 mg/ml. Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

Can be used as standard in a Sandwich ELISA.

FACS

Protein standard in 1D and 2D SDS gel electrophoresis.
Immunoassay.
Antigen for immunization.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above)

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after vimentin is dissolved in 6 M urea buffer (see above)

Protocol: 1. Uehara Y.

Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris

Secreted

Suitable for Immunoblotting (Western or Dot blot)

and Fukazawa H. (1991) Methods Enzymol. 201: 370.
2. Fukazawa H.

and Nuccitelli R. (1992) J.Biol.Chem. 267:5608.

both dissolved in 9.5 M urea buffer (see above).
Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g.Dulbecco's PBS).

et al. (1991) Biochem. Pharmacol. 42: 1661.
3. Satoh T.

et al.(1992) J.Biol.Chem. 267: 2537.
4. Weiss R.

optimal concentration with heparin (30 μg/ml) is about 12 μg/ml.

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl

the solution can be further dialyzed against PBS (Phosphate Buffered Saline

000) as well as other antibody based fluorescent assays.

000)
Flow Cytometry/FACS (1/1

000-1/200

000-1/32

000-1/40

000.

000.
Immunohistochemistry: 1/1

000.
Protein G Peroxidase is a useful reagent in Western Blotting and ELISA experiments. Protein G Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary antibody and appropriate substrate (such as TMB-1000 or Femtomax-110).

000.
Western Blot: 1/500-1/2

000.
Western blot: 1/10

500.
Immunohistochemistry: 1/200-1/1

ELISA.
Western Blot.
Cell culture and/or animal studies.

ELISA.
Suitable for use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

ELISA.
Western Blot.

ELISA.
Western blotting.

ELISA: this protein is suitable for use as a Mouse IgE standard in ELISA assays.

ELISA.

Functional Assays.

Positive control for Western blot analysis

Positive control for Western blot analysis.
Standard for ELISA.

Adermann K. Role of the prosequence of guanylin. Protein Sci. 1999 Sep; 8(9): 1850-9.
4. Lauber T

Cell culture and/or animal studies.
ELISA.
Western Blot.

Cohen MB. Proguanylin secretion and the role of negative-feedback inhibition in a villous epithelial cell line. Am J Physiol Gastrointest Liver Physiol. 2002 Sep; 283(3): G695-702.
3. Schulz A

ELISA and Immunohistochemistry as well as other beta galactosidase-avidin/biotin based enzymatic assays. This product has been assayed against 1.0 µg of biotinylated IgG in a standard capture ELISA using OPNG as a substrate for 30 minutes at room temperature.
Recommended Dilutions:
ELISA: 1/8000-1/32000.
Western Blot: 1/500-1/2500.
Immunohistochemistry: 1/200-1/1000.

ELISA and immunohistochemistry as well as other glucose oxidase-antibody based enzymatic assays.
Recommended Dilutions:
ELISA: 1/8

ELISA.
Functional Assays.

ELISA.
Western blotting.

For use as a Control or Standard in Indirect trapping ELISA for quantitation of antigen in serum using a standard curve

For use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

Forssmann WG

Hawkins JA

Hidaka Y

Immunofluorescence Microscopy (1/1

Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-biotin-avidin based enzymatic assays.
Recommended Dilutions:
ELISA: 1/20

Marx UC

Marx UC. Native and recombinant proguanylin feature identical biophysical properties and are monomeric in solution. Biochemistry. 2002 Dec 10; 41(49): 14602-12.
2. Rudolph JA

Marx UC. Solution structure of human proguanylin: the role of a hormone prosequence. J Biol Chem. 2003 Jun 27; 278(26): 24118-24. Epub 2003 Apr 21.

Neudecker P

Nourse A

Rosch P

Schulz A

Shimonishi Y

Specific methodologies have not been tested using this product.

Suitable for Immunoblotting (Western or dot blot)

Western Blot.
ELISA.

Western Blot.
Protocol: 1. Lauber T

Western Blotting.

change growth medium against basal medium (EBM) in the early afternoon]
- change EGF against EBM (for HUVEC: EBM +1-2% FCS)
- incubate 24 h
- change medium again and add factors (growth factors

etc.)
- incubate for 18 h
- add 10 µl 3H-Thymidine solution [0.025 mCi/ml] per well (=0.25 µCi)
- incubate another 6h at 37°C
- Washing steps: (250 µl/well)
PBS 1x
MeOH 2x 5 min
TCA 2x 10 min
H2O 1x
- lyse cells in 250 µl 0.3M NaOH per well
- transfer 2.5 ml ECO Plus into the appropriate scintillation vials
- transfer cell lysats into the scintillation vials
- count by liquid scintillation (ß-counter; Beckmann Instruments)

for immunoprecipitation and for Wwestern blotting.

inhibitors

optimal concentration with heparin (50 μg/ml) is about 12 μg/ml.
Protocol: Thymidine Incorporation with HUVEC
- plate cells with a density at 5-7 x 10e3 cells/well in growth medium (EGM)
- incubate cells over night [if urgent

plate cells in the morning

Metabolic pathways

Pathways in cancer

MAPK signaling pathway

Cytokine-cytokine receptor interaction

Regulation of actin cytoskeleton

Neuroactive ligand-receptor interaction

Focal adhesion

Endocytosis

Chemokine signaling pathway

Neurotrophin signaling pathway

Calcium signaling pathway

Wnt signaling pathway

Purine metabolism

Huntington's disease

Alzheimer's disease

Ubiquitin mediated proteolysis

Jak-STAT signaling pathway

Insulin signaling pathway

Axon guidance

Natural killer cell mediated cytotoxicity

Cell cycle

Tight junction

Oocyte meiosis

Leukocyte transendothelial migration

Cell adhesion molecules (CAMs)

Progesterone-mediated oocyte maturation

Lysosome

T cell receptor signaling pathway

ErbB signaling pathway

Olfactory transduction

Hematopoietic cell lineage

Toll-like receptor signaling pathway

Systemic lupus erythematosus

Melanogenesis

Spliceosome

GnRH signaling pathway

Prostate cancer

Apoptosis

Fc gamma R-mediated phagocytosis

Vascular smooth muscle contraction

Colorectal cancer

Adherens junction

Parkinson's disease

Oxidative phosphorylation

Dilated cardiomyopathy

Pancreatic cancer

Antigen processing and presentation

Pyrimidine metabolism

Hypertrophic cardiomyopathy (HCM)

Small cell lung cancer

VEGF signaling pathway

Melanoma

TGF-beta signaling pathway

B cell receptor signaling pathway

Fc epsilon RI signaling pathway

Chronic myeloid leukemia

Gap junction

Arrhythmogenic right ventricular cardiomyopathy (ARVC)

Renal cell carcinoma

Glioma

Acute myeloid leukemia

Adipocytokine signaling pathway

PPAR signaling pathway

Complement and coagulation cascades

p53 signaling pathway

Long-term potentiation

Ribosome

Phosphatidylinositol signaling system

Glycolysis / Gluconeogenesis

Epithelial cell signaling in Helicobacter pylori infection

RIG-I-like receptor signaling pathway

Long-term depression

NOD-like receptor signaling pathway

Cardiac muscle contraction

Non-small cell lung cancer

Pathogenic Escherichia coli infection

Endometrial cancer

Viral myocarditis

Glycerophospholipid metabolism

ECM-receptor interaction

Drug metabolism - cytochrome P450

Metabolism of xenobiotics by cytochrome P450

Vibrio cholerae infection

Amyotrophic lateral sclerosis (ALS)

Arginine and proline metabolism

Cytosolic DNA-sensing pathway

Valine

mTOR signaling pathway

Basal cell carcinoma

Hedgehog signaling pathway

Inositol phosphate metabolism

Retinol metabolism

Thyroid cancer

Drug metabolism - other enzymes

Glutathione metabolism

Bladder cancer

RNA degradation

leucine and isoleucine degradation

Amino sugar and nucleotide sugar metabolism

Arachidonic acid metabolism

Type II diabetes mellitus

Fatty acid metabolism

Proteasome

Autoimmune thyroid disease

Starch and sucrose metabolism

Glycerolipid metabolism

Pyruvate metabolism

Nucleotide excision repair

Notch signaling pathway

Tryptophan metabolism

Tyrosine metabolism

Lysine degradation

Base excision repair

Taste transduction

Type I diabetes mellitus

SNARE interactions in vesicular transport

Sphingolipid metabolism

Primary immunodeficiency

Fructose and mannose metabolism

Graft-versus-host disease

Alanine

aspartate and glutamate metabolism

Butanoate metabolism

Cysteine and methionine metabolism

N-Glycan biosynthesis

Prion diseases

Glycosphingolipid biosynthesis - lacto and neolacto series

Propanoate metabolism

Allograft rejection

Aminoacyl-tRNA biosynthesis

Androgen and estrogen metabolism

Citrate cycle (TCA cycle)

DNA replication

Pentose phosphate pathway

Porphyrin and chlorophyll metabolism

Ether lipid metabolism

Glycine

Regulation of autophagy

serine and threonine metabolism

Histidine metabolism

Nitrogen metabolism

Selenoamino acid metabolism

Galactose metabolism

O-Glycan biosynthesis

Basal transcription factors

Dorso-ventral axis formation

Linoleic acid metabolism

Nicotinate and nicotinamide metabolism

Asthma

Homologous recombination

Maturity onset diabetes of the young

RNA polymerase

beta-Alanine metabolism

Glycosaminoglycan degradation

Sulfur metabolism

Mismatch repair

Phenylalanine metabolism

Pantothenate and CoA biosynthesis

Primary bile acid biosynthesis

Biosynthesis of unsaturated fatty acids

Glycosylphosphatidylinositol(GPI)-anchor biosynthesis

Heparan sulfate biosynthesis

Riboflavin metabolism

Pentose and glucuronate interconversions

Keratan sulfate biosynthesis

Other glycan degradation

Renin-angiotensin system

Terpenoid backbone biosynthesis

Ascorbate and aldarate metabolism

One carbon pool by folate

alpha-Linolenic acid metabolism

Chondroitin sulfate biosynthesis

Glycosphingolipid biosynthesis - globo series

Glyoxylate and dicarboxylate metabolism

leucine and isoleucine biosynthesis

Limonene and pinene degradation

ABC transporters

C21-Steroid hormone metabolism

Circadian rhythm - mammal

Glycosphingolipid biosynthesis - ganglio series

Non-homologous end-joining

Steroid biosynthesis

Taurine and hypotaurine metabolism

Caffeine metabolism

Folate biosynthesis

Thiamine metabolism

Cyanoamino acid metabolism

Fatty acid elongation in mitochondria

Synthesis and degradation of ketone bodies

Protein export

Ubiquinone and other terpenoid-quinone biosynthesis

Fatty acid biosynthesis

Methane metabolism

Lipoic acid metabolism

D-Glutamine and D-glutamate metabolism

Lysine biosynthesis

Phenylalanine

Vitamin B6 metabolism

tyrosine and tryptophan biosynthesis

O-Mannosyl glycan biosynthesis

Biotin metabolism

D-Arginine and D-ornithine metabolism

1

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