Products

cDNA Clones

Antibodies

Proteins

Expression Plasmids

Primary Antibodies

Recombinant Proteins

CytoSections

Mutant ORF Clones

Mass Spec Standards

Over-expression Lysates

Mutant Proteins

cDNA Clone Sets

Lentiviral Particles

Human

BRCA1

P53

MENIN

BETA CATENIN

CREM

CONSTITUTIVE ANDROSTANE RECEPTOR

NRG1

WILMS TUMOR PROTEIN

MORTALITY FACTOR 4 LIKE 2

B7-1

GLUCOCORTICOID RECEPTOR

HNF 4 ALPHA

ERG

ESTROGEN RELATED RECEPTOR GAMMA

IRF5

TGIF

P63

C-JUN

IKAROS

P73

ESTROGEN RECEPTOR 1

PLAGL1

ATAXIN 3

RUNX1T1

MBD1

PQBP1

IRF3

HMGA1

IKK GAMMA

PML PROTEIN

MAX

MEF2C

FOS B

TCF7L2

HDAC9

MEIS2

MDM2

APE1

BMAL1

FOXP1

GABPB2

HDAC8

MITF

PRKCBP1

SQSTM1

C FOS

FE65

GROWTH ARREST SPECIFIC PROTEIN 7

PAX5

UBE2V1

ESTROGEN RECEPTOR BETA

NONO

CAMKK2

NCOA4

C-MYC

NFAT2

PAX3

TNF ALPHA

BCL 6

HIF1 BETA

RNF14

SATB1

ID3

OCT4

STAT4

TCF4

TTF1

AREB6

BORIS

EWSR1

PPAR GAMMA

RBPJK

SCMH1

STAT1

XPD

ZNF133

FOXI1

NFAT1

PRRX1

ZFP200

CNOT4

RETINOIC ACID RECEPTOR ALPHA

SOX5

ZNF302

PROGESTERONE RECEPTOR

PTGER3

ZNF447

BRG1

C-MYB

ATF3

CDX2

GLI1

HNRNPD

RHOH

SOX17

SSB

SSX1

THYROID HORMONE RECEPTOR ALPHA

ZNF83

FOXP3

Human

Mouse

Rat

Dog

Monkey

Human (Expected from sequence similarity: Mouse

Cow)

Bovine

Pig

Human (Predicted: Mouse

Canine

Chicken

Dog)

Primate

Bovine)

Pig)

Hamster

Human (Predicted: Mouse)

Zebrafish

Porcine

Rat)

Rabbit

Xenopus

Mouse (Expected from sequence similarity: Rat

Cow

Mouse (Predicted: Rat

Sheep

Human (Expected from sequence similarity: Mouse)

Xenopus)

Equine

Human (Predicted: Rat

Bat

Chimpanzee

Horse

Human (Expected from sequence similarity: Dog

Human (Predicted: Bovine)

Human (Expected from sequence similarity: Rat

Rat (Expected from sequence similarity: Dog

Chicken)

Feline

Human (Expected from sequence similarity: Dog)

Mouse)

Rabbit)

X. tropicalis)

Gorilla

Rhesus Monkey

Rhesus Monkey)

Drosophila

Gibbon

Guinea Pig

Human (Predicted: Rat)

Human (Predicted: Rhesus Monkey)

Macaque

Mouse (Predicted: Bovine)

Mouse (Predicted: Rat)

Rat (Expected from sequence similarity: Mouse

Zebrafish)

African clawed frog

Ferret

Goat

Hamster)

Human (Expected from sequence similarity: Cow)

Human (Predicted: Bovine

Human (Predicted: Monkey)

Human (Predicted: Pig)

Mammalian

Mouse (Expected from sequence similarity: Human

Rat (Expected from sequence similarity: Dog)

X. tropicalis

Fish

Horse)

Human (Expected from sequence similarity: Pig

Human (Predicted: Monkey

Mouse (Expected from sequence similarity: Dog

Mouse (Expected from sequence similarity: Dog)

Mouse (Expected from sequence similarity: Rat)

Mouse (Predicted: Bovine

Mouse (Predicted: Chicken

Mouse (Predicted: Pig

Rat (Expected from sequence similarity: Cow

Rat (Expected from sequence similarity: Human

Rat (Predicted: Mouse)

C. elegans

Chimpanzee)

Expected from sequence similarity: Human

Gerbil

Gibbon (Predicted: Monkey

Golden Syrian Hamster

Guinea Pig)

Hamster (Predicted: Bovine)

Horse (Predicted: Bat

Horse (Predicted: Monkey)

Horse (Predicted: Mouse

Horse (Predicted: Xenopus)

Human (Expected from sequence similarity: Feline

Human (Expected from sequence similarity: Pig)

Human (Expected from sequence similarity: Rat)

Human (Predicted: Bat

Human (Predicted: Chicken

Human (Predicted: Chimpanzee)

Human (Predicted: Cow

Human (Predicted: Hamster

Human (Predicted: Pig

Human (Predicted: Rhesus Monkey

Human (Predicted: Zebrafish

Monkey (Predicted: Rat

Monkey)

Mouse (Expected from sequence similarity: Human)

Mouse (Predicted: Chicken)

Mouse (Predicted: Chimpanzee

Mouse (Predicted: Chimpanzee)

Mouse (Predicted: Monkey

Mouse (Predicted: Rhesus Monkey)

Mouse (Predicted: Zebrafish)

Opossum

Pig (Expected from sequence similarity: Cow)

Pig (Expected from sequence similarity: Dog

Pig (Expected from sequence similarity: Human

Pig (Expected from sequence similarity: Rat

Porcine (Does not react with: Mouse)

Pufferfish

Rat (Expected from sequence similarity: Cow)

Rat (Predicted: Bovine

Rat (Predicted: Mouse

Saccharomyces cerevisiae

Sheep)

Turkey

Yeast

Yeast (Does not react with: Xenopus)

WB

IHC

IF

FC

ELISA

ICC/IF

IP

LMNX

Simple Western

10k-ChIP

ChIP

Immunoblotting

PEP-ELISA

Dot

CyTOF-ready

FN

SISCAPA

ICC

Block/Neutralize

RIP

EMSA

Electron Microscopy

ID

Protein Array

SDS-PAGE

Sandwich ELISA

Mouse

Rabbit

Goat

Sheep

Hamster

Rat

Chicken

Guinea Pig

Human

HEK293T

HEK293

E. coli

Sf9

CHO

Insect

Plasmids

Particles

Myc-DDK

Tag Free

TurboGFP

mGFP

C-Myc/DDK

C-MYC/DDK

N-His

N-GST and C-His

C-DDK

N-GST and C-HIS

C-His

His-tag

N-His-ABP (Albumin-Binding Protein)

N-6His

N-GST

C-DDK/His

N-GST, C-His

N-His-GST

C-6×His

C-Fc/His

C-Mouse Fc and 6×His

N-6His-FLAG

N-His/DDK

Tag-free

pCMV6-Entry

pCMV6-AC-GFP

pLenti-C-Myc-DDK-P2A-Puro

pLenti-C-mGFP-P2A-Puro

pCMV6-XL5

pLenti-C-Myc-DDK

pLenti-C-mGFP

pCMV6-XL4

pCMV6-AC

pCMV6-AC-Myc-DDK

pCMV6-XL6

PCMV6-Neo

pCMV6-AN-Myc-DDK

KO Validated Antibodies

Unconjugated

Biotin

HRP

FITC

DyLight 488

PE

Phospho-specific

IgG1

IgG

IgG2a

IgG2b

Ig

IgG1, kappa

IgG3

IgG2b, kappa

IgG2a, kappa

IgY

IgG3, kappa

IgG1/IgK

IgG2, alpha

IgM

IgM, kappa

Monoclonal

Polyclonal

No

Yes

Indirect

Sandwich

Direct

LentiORF

TrueORF

TrueMAB

TrueClone

MVPro

CytoSections

TrueORF Gold, TrueORF

UltraMAB

TrueRAB

SDS PAGE

Druggable Genome

Transmembrane

WB

Transcription Factors

Secreted Protein

Protein Kinase

ES Cell Differentiation/IPS

Protease

Stem cell - Pluripotency

GPCR

Phosphatase

Adult stem cells

Ion Channels: Other

Embryonic stem cells

Nuclear Hormone Receptor

Cancer stem cells

Ion Channels: Potassium

Induced pluripotent stem cells

P450

Stem cell relevant signaling - JAK/STAT signaling pathway

Ion Channels: Cys-loop Receptors

Stem cell relevant signaling - TGFb/BMP signaling pathway

Stem cell relevant signaling - DSL/Notch pathway

Ion Channels: Transient receptor potential

Stem cell relevant signaling - Wnt Signaling pathway

Ion Channels: Glutamate Receptors

Ion Channels: Calcium

Ion Channels: Sodium

Ion Channels: ATP Receptors

Ion Channels: Cyclic nucleotide gated

ELISA

SDS-PAGE

Bioactivity

the solution can be further dialyzed against PBS (phosphate buffered saline

10 mM Tris-HCl

2 mM dithiothreitol

Enzyme Activity

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g. Dulbecco's PBS).

ELISA.
Western Blot.

Flow Cyt

pH 7.4). For immunization purposes

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.

pH 7.4).
For immunization purposes

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

e.g. Dulbeccos PBS).

e.g. Dulbeccos PBS).
See References 2 and 3 for more details.

pH 7.4).
For immunization purposes

pH 7.4).
For Immunization purposes

Biological activity/ Working concentration: Optimum concentration for human umbilical vein endothelial cells (HUVEC) range from 50-200 μg/ml

ELISA.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoblotting.
Receptor-binding studies.

Western Blot.

0.1% (w/v) insulin

000-1/5

10 mM sodium phosphate

10mM Tris-HCl

1mM DTT

2 mM dithiothreitol

2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes

2mM dithiothreitol

63mM sodium phosphate

Biological activity/ Working concentration: The working concentration of ECGF for HUVEC is in the range of 25µg/ml to 100µg/ml. When adding Heparin (2.5mg per mg ECGF) an ECGF concentration of 12µg/ml (30µg/ml Heparin) is optimal. In this case 6mg ECGF are sufficient for 500 ml medium.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above)

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to Filaments is performed by dissolving in 6 M urea buffer (see above) at concentrations of approx. 0.5 mg/ml. Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

Can be used as standard in a Sandwich ELISA.

FACS

Protein standard in 1D and 2D SDS gel electrophoresis.
Immunoassay.
Antigen for immunization.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above)

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after vimentin is dissolved in 6 M urea buffer (see above)

Protocol: 1. Uehara Y.

Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris

Secreted

Suitable for Immunoblotting (Western or Dot blot)

and Fukazawa H. (1991) Methods Enzymol. 201: 370.
2. Fukazawa H.

and Nuccitelli R. (1992) J.Biol.Chem. 267:5608.

both dissolved in 9.5 M urea buffer (see above).
Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g.Dulbecco's PBS).

et al. (1991) Biochem. Pharmacol. 42: 1661.
3. Satoh T.

et al.(1992) J.Biol.Chem. 267: 2537.
4. Weiss R.

optimal concentration with heparin (30 μg/ml) is about 12 μg/ml.

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl

the solution can be further dialyzed against PBS (Phosphate Buffered Saline

000) as well as other antibody based fluorescent assays.

000)
Flow Cytometry/FACS (1/1

000-1/200

000-1/32

000-1/40

000.

000.
Immunohistochemistry: 1/1

000.
Protein G Peroxidase is a useful reagent in Western Blotting and ELISA experiments. Protein G Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary antibody and appropriate substrate (such as TMB-1000 or Femtomax-110).

000.
Western Blot: 1/500-1/2

000.
Western blot: 1/10

500.
Immunohistochemistry: 1/200-1/1

ELISA.
Western Blot.
Cell culture and/or animal studies.

ELISA.
Suitable for use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

ELISA.
Western Blot.

ELISA.
Western blotting.

ELISA: this protein is suitable for use as a Mouse IgE standard in ELISA assays.

ELISA.

Functional Assays.

Positive control for Western blot analysis

Positive control for Western blot analysis.
Standard for ELISA.

Adermann K. Role of the prosequence of guanylin. Protein Sci. 1999 Sep; 8(9): 1850-9.
4. Lauber T

Cell culture and/or animal studies.
ELISA.
Western Blot.

Cohen MB. Proguanylin secretion and the role of negative-feedback inhibition in a villous epithelial cell line. Am J Physiol Gastrointest Liver Physiol. 2002 Sep; 283(3): G695-702.
3. Schulz A

ELISA and Immunohistochemistry as well as other beta galactosidase-avidin/biotin based enzymatic assays. This product has been assayed against 1.0 µg of biotinylated IgG in a standard capture ELISA using OPNG as a substrate for 30 minutes at room temperature.
Recommended Dilutions:
ELISA: 1/8000-1/32000.
Western Blot: 1/500-1/2500.
Immunohistochemistry: 1/200-1/1000.

ELISA and immunohistochemistry as well as other glucose oxidase-antibody based enzymatic assays.
Recommended Dilutions:
ELISA: 1/8

ELISA.
Functional Assays.

ELISA.
Western blotting.

For use as a Control or Standard in Indirect trapping ELISA for quantitation of antigen in serum using a standard curve

For use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

Forssmann WG

Hawkins JA

Hidaka Y

Immunofluorescence Microscopy (1/1

Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-biotin-avidin based enzymatic assays.
Recommended Dilutions:
ELISA: 1/20

Marx UC

Marx UC. Native and recombinant proguanylin feature identical biophysical properties and are monomeric in solution. Biochemistry. 2002 Dec 10; 41(49): 14602-12.
2. Rudolph JA

Marx UC. Solution structure of human proguanylin: the role of a hormone prosequence. J Biol Chem. 2003 Jun 27; 278(26): 24118-24. Epub 2003 Apr 21.

Neudecker P

Nourse A

Rosch P

Schulz A

Shimonishi Y

Specific methodologies have not been tested using this product.

Suitable for Immunoblotting (Western or dot blot)

Western Blot.
ELISA.

Western Blot.
Protocol: 1. Lauber T

Western Blotting.

change growth medium against basal medium (EBM) in the early afternoon]
- change EGF against EBM (for HUVEC: EBM +1-2% FCS)
- incubate 24 h
- change medium again and add factors (growth factors

etc.)
- incubate for 18 h
- add 10 µl 3H-Thymidine solution [0.025 mCi/ml] per well (=0.25 µCi)
- incubate another 6h at 37°C
- Washing steps: (250 µl/well)
PBS 1x
MeOH 2x 5 min
TCA 2x 10 min
H2O 1x
- lyse cells in 250 µl 0.3M NaOH per well
- transfer 2.5 ml ECO Plus into the appropriate scintillation vials
- transfer cell lysats into the scintillation vials
- count by liquid scintillation (ß-counter; Beckmann Instruments)

for immunoprecipitation and for Wwestern blotting.

inhibitors

optimal concentration with heparin (50 μg/ml) is about 12 μg/ml.
Protocol: Thymidine Incorporation with HUVEC
- plate cells with a density at 5-7 x 10e3 cells/well in growth medium (EGM)
- incubate cells over night [if urgent

plate cells in the morning

Pathways in cancer

MAPK signaling pathway

Wnt signaling pathway

Prostate cancer

Cell cycle

Chronic myeloid leukemia

Huntington's disease

Colorectal cancer

Ubiquitin mediated proteolysis

Neurotrophin signaling pathway

Toll-like receptor signaling pathway

Thyroid cancer

Small cell lung cancer

Pancreatic cancer

Acute myeloid leukemia

TGF-beta signaling pathway

T cell receptor signaling pathway

Endometrial cancer

Apoptosis

Basal cell carcinoma

Metabolic pathways

B cell receptor signaling pathway

Adipocytokine signaling pathway

p53 signaling pathway

Bladder cancer

Purine metabolism

Pyrimidine metabolism

Melanoma

Non-small cell lung cancer

Melanogenesis

Adherens junction

Amyotrophic lateral sclerosis (ALS)

Cytosolic DNA-sensing pathway

Jak-STAT signaling pathway

Basal transcription factors

Chemokine signaling pathway

RNA polymerase

Maturity onset diabetes of the young

Glioma

ErbB signaling pathway

Renal cell carcinoma

RIG-I-like receptor signaling pathway

Tight junction

Focal adhesion

Natural killer cell mediated cytotoxicity

Arrhythmogenic right ventricular cardiomyopathy (ARVC)

Systemic lupus erythematosus

Endocytosis

NOD-like receptor signaling pathway

RNA degradation

Oocyte meiosis

Epithelial cell signaling in Helicobacter pylori infection

Insulin signaling pathway

Nucleotide excision repair

Axon guidance

Notch signaling pathway

VEGF signaling pathway

Antigen processing and presentation

Neuroactive ligand-receptor interaction

Leukocyte transendothelial migration

PPAR signaling pathway

Viral myocarditis

Spliceosome

Allograft rejection

Graft-versus-host disease

Type I diabetes mellitus

Circadian rhythm - mammal

Pathogenic Escherichia coli infection

Alzheimer's disease

GnRH signaling pathway

Autoimmune thyroid disease

Cell adhesion molecules (CAMs)

DNA replication

Primary immunodeficiency

Type II diabetes mellitus

Cytokine-cytokine receptor interaction

Fc epsilon RI signaling pathway

Dorso-ventral axis formation

Base excision repair

Dilated cardiomyopathy

Hypertrophic cardiomyopathy (HCM)

Lysine degradation

Fc gamma R-mediated phagocytosis

Calcium signaling pathway

Long-term potentiation

Long-term depression

mTOR signaling pathway

Hedgehog signaling pathway

Ribosome

Asthma

Hematopoietic cell lineage

Regulation of actin cytoskeleton

Progesterone-mediated oocyte maturation

Cysteine and methionine metabolism

Homologous recombination

Valine

Proteasome

Non-homologous end-joining

Glycolysis / Gluconeogenesis

Prion diseases

Vascular smooth muscle contraction

Aminoacyl-tRNA biosynthesis

Ether lipid metabolism

leucine and isoleucine biosynthesis

Glycerolipid metabolism

Glycine

Glyoxylate and dicarboxylate metabolism

serine and threonine metabolism

Nicotinate and nicotinamide metabolism

SNARE interactions in vesicular transport

leucine and isoleucine degradation

Mismatch repair

Biosynthesis of unsaturated fatty acids

E. coli

Human

Human 293

96-well plate, Luminex

Luminex

96-well plate

Neomycin

Puromycin

None

TA600183

TA700050

TA600050

TA600306

TA600350

TA600545

TA700170

TA700174

TA700304

TA600042

TA600268

TA600269

TA700269

TA700302

TA700484

TA700489

TA700523

TA600003

TA600004

TA600005

TA600021

TA600026

TA600034

TA600035, TA600034

TA600035, TA600047, TA600141

TA600047

TA600065

TA600065, TA600066

TA600066, TA600067

TA600103, TA600104

TA600104, TA600103

TA600105

TA600106

TA600107

TA600108

TA600123

TA600123, TA600125

TA600124

TA600124, TA600126

TA600170, TA600171, TA600172

TA600173

TA600174

TA600174, TA600175, TA600178, TA600179

TA600176

TA600176, TA600177

TA600268, TA600269, TA600271

TA600268, TA600270, TA600272

TA600302

TA600302, TA600304, TA600305

TA600303, TA600307, TA600308

TA600319

TA600381

TA600382

TA600383

TA600384

TA600385

TA600386

TA600387

TA600388

TA600429

TA600430

TA600431

TA600482

TA600482, TA600484

TA600483, TA600485, TA600484

TA600486

TA600489

TA600490, TA600491

TA600496

TA600497

TA600523, TA600524

TA700003

TA700004

TA700005

TA700021

TA700026

TA700034, TA700035

TA700034, TA700141

TA700035

TA700035, TA700047

TA700042, TA700046

TA700065

TA700065, TA700066

TA700066, TA700067

TA700103, TA700104

TA700104, TA700103

TA700105

TA700106

TA700107

TA700108

TA700123

TA700123, TA700125

TA700124

TA700124, TA700126

TA700171

TA700174, TA700175

TA700176

TA700176, TA700177

TA700183, TA700192, TA700193, TA700194, TA700195

TA700268

TA700268, TA700269, TA700270, TA700271

TA700268, TA700272, TA700293

TA700302, TA700303

TA700305, TA700306, TA700307

TA700350, TA700362, TA700363

TA700381

TA700382

TA700383

TA700384

TA700385

TA700386

TA700387

TA700388

TA700429

TA700430

TA700431

TA700482, TA700483

TA700483, TA700484

TA700486

TA700490

TA700496

TA700497

TA700522

TA700546, TA700547, TA700548

Carrier-free Antibodies

1

2

3

Yes

In Stock