Products

cDNA Clones

Antibodies

Proteins

Expression Plasmids

Primary Antibodies

Recombinant Proteins

CytoSections

Mass Spec Standards

Mutant Proteins

Mutant ORF Clones

Native Proteins

Human

CD10

EPOXIDE HYDROLASE

LEUKOTRIENE A4 HYDROLASE

PSMA

PGP9.5

FLIP

MONOACYLGLYCEROL LIPASE

AMINOACYLASE 1

HAPTOGLOBIN

CASPASE 3

TMPRSS4

CASPASE-7

PROSTATE SPECIFIC ANTIGEN

CATHEPSIN B

CATHEPSIN L

HGF

CASPASE 1

PACE4

PSMA4

ARTS1

DPP3

DPP9

OTUB1

SENP8

USP5

ADAM15

CASPASE 8

CASPASE 9

ECE1

SIL1

C2

CARBOXYPEPTIDASE M

DPP10

MALT1

MMP13

MMP2

PROTEASOME SUBUNIT ALPHA TYPE 6

OTUB2

AMINOPEPTIDASE A

CD13

KALLIKREIN 11

MMP3

TMPRSS5

DPP6

UPA

BACE1

C1S

CLPP

FACTOR I

L KYNURENINE HYDROLASE

USP16

CALPAIN 3

CALPAIN 9

CD71

DPP8

PSMB9

USP7

KALLIKREIN 6

PREPL

USP44

ATG4B

NAPSIN A

PEPD

SENP2

SPG7

ANGIOTENSIN CONVERTING ENZYME 1

CASPASE 5

FACTOR X

MMP7

NLN

PARK7

PHOSPHORIBOSYL PYROPHOSPHATE AMIDOTRANSFERASE

SEC11A

XPNPEP3

LACTOFERRIN

MEST

PEPSINOGEN II

ATP DEPENDENT METALLOPROTEASE YME1L1

CASPASE 10

GGT1

USP20

AGTPBP1

MASP2

OTUD5

PSMA1

USP8

CALPAIN 2

CASPASE 4

CNDP1

CNDP2

FACTOR VII

PROTEIN Z

CARBOXYPEPTIDASE A2

CTSA

PLAT

TRANSFERRIN RECEPTOR 2

XPNPEP1

ADAM22

ADAMTS1

ADAMTS4

Human

Mouse

Rat

Monkey

Dog

Bovine

Pig

Bovine)

Cow)

Human (Expected from sequence similarity: Mouse

Human (Predicted: Mouse

Canine

Rabbit

Hamster

Mouse (Predicted: Rat

Porcine

Primate

Rat)

Zebrafish

Gorilla

Dog)

Gerbil

Gibbon

Human (Expected from sequence similarity: Dog

Human (Predicted: Rat

Rabbit)

Xenopus

Bat

Cow

Feline

Horse

Human (Predicted: Monkey

Rhesus Monkey)

X. tropicalis)

Chicken

Chimpanzee

Horse)

Human (Expected from sequence similarity: Mouse)

Human (Expected from sequence similarity: Rat

Human (Predicted: Chimpanzee)

Human (Predicted: Dog

Human (Predicted: Dog)

Human (Predicted: Monkey)

Monkey)

Mouse (Predicted: Monkey)

Pig)

Rat (Predicted: Mouse

Chicken)

Equine

Fish

Guinea Pig

Hamster)

Horse (Predicted: Pig)

Human (Expected from sequence similarity: Dog)

Human (Predicted: Bovine

Human (Predicted: Bovine)

Human (Predicted: Chicken

Human (Predicted: Hamster)

Human (Predicted: Mouse)

Human (Predicted: Pig

Human (Predicted: Rabbit

Human (Predicted: Rabbit)

Mouse (Expected from sequence similarity: Human

Mouse (Predicted: Chicken

Mouse (Predicted: Chimpanzee

Mouse (Predicted: Rat)

Mouse (Predicted: Xenopus

Mouse)

Orang-Utan

Orang-Utan (Predicted: Bat

Orang-Utan (Predicted: Hamster

Orang-Utan (Predicted: Horse)

Pig (Expected from sequence similarity: Dog)

Rat (Expected from sequence similarity: Cow

Rat (Expected from sequence similarity: Dog)

Rat (Expected from sequence similarity: Human)

Rat (Expected from sequence similarity: Pig

Rat (Predicted: Rabbit)

Rhesus Monkey

Sheep

Sheep)

Xenopus)

Yeast

WB

IHC

FC

IF

ELISA

IP

LMNX

ICC/IF

10k-ChIP

ID

Simple Western

R

PEP-ELISA

CyTOF-ready

FN

Immunoblotting

SISCAPA

ChIP

Electron Microscopy

ICC

Neutralize

Mouse

Rabbit

Goat

Chicken

Guinea Pig

Sheep

HEK293T

HEK293

E. coli

Sf9

Insect

Plasmids

Myc-DDK

mGFP

Tag Free

C-Myc/DDK

TurboGFP

C-MYC/DDK

N-His

N-GST and C-His

C-His

N-GST and C-HIS

C-DDK

C-HIS

C-HUMAN

N-6His

N-GST, C-His

N-His-PDCD1(Pro21-Val170)

N-His/DDK

pCMV6-Entry

pCMV6-AC-GFP

pLenti-C-Myc-DDK-P2A-Puro

pLenti-C-mGFP-P2A-Puro

pLenti-C-Myc-DDK

pLenti-C-mGFP

pCMV6-XL5

pCMV6-XL4

pCMV6-AC

pCMV6-XL6

PCMV6-Neo

KO Validated Antibodies

Unconjugated

Biotin

HRP

FITC

PE

APC

TRITC

Phospho-specific

IgG1

IgG

IgG2a

IgG2b

Ig

IgG1, kappa

IgG2a, kappa

IgG3

IgM

IgY

Monoclonal

Polyclonal

No

Yes

Indirect

Sandwich

Direct

LentiORF

TrueMAB

TrueORF

TrueClone

MVPro

CytoSections

TrueORF Gold, TrueORF

UltraMAB

TrueRAB

SDS PAGE

Druggable Genome

Transmembrane

WB

Transcription Factors

Secreted Protein

Protein Kinase

ES Cell Differentiation/IPS

Protease

Stem cell - Pluripotency

GPCR

Phosphatase

Adult stem cells

Ion Channels: Other

Embryonic stem cells

Nuclear Hormone Receptor

Cancer stem cells

Ion Channels: Potassium

Induced pluripotent stem cells

P450

Stem cell relevant signaling - JAK/STAT signaling pathway

Ion Channels: Cys-loop Receptors

Stem cell relevant signaling - TGFb/BMP signaling pathway

Stem cell relevant signaling - DSL/Notch pathway

Ion Channels: Transient receptor potential

Stem cell relevant signaling - Wnt Signaling pathway

Ion Channels: Glutamate Receptors

Ion Channels: Calcium

Ion Channels: Sodium

Ion Channels: ATP Receptors

Ion Channels: Cyclic nucleotide gated

ELISA

SDS-PAGE

Bioactivity

the solution can be further dialyzed against PBS (phosphate buffered saline

10 mM Tris-HCl

2 mM dithiothreitol

Enzyme Activity

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g. Dulbecco's PBS).

ELISA.
Western Blot.

Flow Cyt

pH 7.4). For immunization purposes

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.

pH 7.4).
For immunization purposes

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

e.g. Dulbeccos PBS).

e.g. Dulbeccos PBS).
See References 2 and 3 for more details.

pH 7.4).
For immunization purposes

pH 7.4).
For Immunization purposes

Biological activity/ Working concentration: Optimum concentration for human umbilical vein endothelial cells (HUVEC) range from 50-200 μg/ml

ELISA.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoblotting.
Receptor-binding studies.

Western Blot.

0.1% (w/v) insulin

000-1/5

10 mM sodium phosphate

10mM Tris-HCl

1mM DTT

2 mM dithiothreitol

2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes

2mM dithiothreitol

63mM sodium phosphate

Biological activity/ Working concentration: The working concentration of ECGF for HUVEC is in the range of 25µg/ml to 100µg/ml. When adding Heparin (2.5mg per mg ECGF) an ECGF concentration of 12µg/ml (30µg/ml Heparin) is optimal. In this case 6mg ECGF are sufficient for 500 ml medium.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above)

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to Filaments is performed by dissolving in 6 M urea buffer (see above) at concentrations of approx. 0.5 mg/ml. Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

Can be used as standard in a Sandwich ELISA.

FACS

Protein standard in 1D and 2D SDS gel electrophoresis.
Immunoassay.
Antigen for immunization.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above)

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after vimentin is dissolved in 6 M urea buffer (see above)

Protocol: 1. Uehara Y.

Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris

Secreted

Suitable for Immunoblotting (Western or Dot blot)

and Fukazawa H. (1991) Methods Enzymol. 201: 370.
2. Fukazawa H.

and Nuccitelli R. (1992) J.Biol.Chem. 267:5608.

both dissolved in 9.5 M urea buffer (see above).
Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g.Dulbecco's PBS).

et al. (1991) Biochem. Pharmacol. 42: 1661.
3. Satoh T.

et al.(1992) J.Biol.Chem. 267: 2537.
4. Weiss R.

optimal concentration with heparin (30 μg/ml) is about 12 μg/ml.

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl

the solution can be further dialyzed against PBS (Phosphate Buffered Saline

000) as well as other antibody based fluorescent assays.

000)
Flow Cytometry/FACS (1/1

000-1/200

000-1/32

000-1/40

000.

000.
Immunohistochemistry: 1/1

000.
Protein G Peroxidase is a useful reagent in Western Blotting and ELISA experiments. Protein G Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary antibody and appropriate substrate (such as TMB-1000 or Femtomax-110).

000.
Western Blot: 1/500-1/2

000.
Western blot: 1/10

500.
Immunohistochemistry: 1/200-1/1

ELISA.
Western Blot.
Cell culture and/or animal studies.

ELISA.
Suitable for use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

ELISA.
Western Blot.

ELISA.
Western blotting.

ELISA: this protein is suitable for use as a Mouse IgE standard in ELISA assays.

ELISA.

Functional Assays.

Positive control for Western blot analysis

Positive control for Western blot analysis.
Standard for ELISA.

Adermann K. Role of the prosequence of guanylin. Protein Sci. 1999 Sep; 8(9): 1850-9.
4. Lauber T

Cell culture and/or animal studies.
ELISA.
Western Blot.

Cohen MB. Proguanylin secretion and the role of negative-feedback inhibition in a villous epithelial cell line. Am J Physiol Gastrointest Liver Physiol. 2002 Sep; 283(3): G695-702.
3. Schulz A

ELISA and Immunohistochemistry as well as other beta galactosidase-avidin/biotin based enzymatic assays. This product has been assayed against 1.0 µg of biotinylated IgG in a standard capture ELISA using OPNG as a substrate for 30 minutes at room temperature.
Recommended Dilutions:
ELISA: 1/8000-1/32000.
Western Blot: 1/500-1/2500.
Immunohistochemistry: 1/200-1/1000.

ELISA and immunohistochemistry as well as other glucose oxidase-antibody based enzymatic assays.
Recommended Dilutions:
ELISA: 1/8

ELISA.
Functional Assays.

ELISA.
Western blotting.

For use as a Control or Standard in Indirect trapping ELISA for quantitation of antigen in serum using a standard curve

For use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

Forssmann WG

Hawkins JA

Hidaka Y

Immunofluorescence Microscopy (1/1

Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-biotin-avidin based enzymatic assays.
Recommended Dilutions:
ELISA: 1/20

Marx UC

Marx UC. Native and recombinant proguanylin feature identical biophysical properties and are monomeric in solution. Biochemistry. 2002 Dec 10; 41(49): 14602-12.
2. Rudolph JA

Marx UC. Solution structure of human proguanylin: the role of a hormone prosequence. J Biol Chem. 2003 Jun 27; 278(26): 24118-24. Epub 2003 Apr 21.

Neudecker P

Nourse A

Rosch P

Schulz A

Shimonishi Y

Specific methodologies have not been tested using this product.

Suitable for Immunoblotting (Western or dot blot)

Western Blot.
ELISA.

Western Blot.
Protocol: 1. Lauber T

Western Blotting.

change growth medium against basal medium (EBM) in the early afternoon]
- change EGF against EBM (for HUVEC: EBM +1-2% FCS)
- incubate 24 h
- change medium again and add factors (growth factors

etc.)
- incubate for 18 h
- add 10 µl 3H-Thymidine solution [0.025 mCi/ml] per well (=0.25 µCi)
- incubate another 6h at 37°C
- Washing steps: (250 µl/well)
PBS 1x
MeOH 2x 5 min
TCA 2x 10 min
H2O 1x
- lyse cells in 250 µl 0.3M NaOH per well
- transfer 2.5 ml ECO Plus into the appropriate scintillation vials
- transfer cell lysats into the scintillation vials
- count by liquid scintillation (ß-counter; Beckmann Instruments)

for immunoprecipitation and for Wwestern blotting.

inhibitors

optimal concentration with heparin (50 μg/ml) is about 12 μg/ml.
Protocol: Thymidine Incorporation with HUVEC
- plate cells with a density at 5-7 x 10e3 cells/well in growth medium (EGM)
- incubate cells over night [if urgent

plate cells in the morning

Complement and coagulation cascades

Alzheimer's disease

Metabolic pathways

Proteasome

Apoptosis

Lysosome

Pathways in cancer

Renin-angiotensin system

Parkinson's disease

Huntington's disease

Amyotrophic lateral sclerosis (ALS)

Hematopoietic cell lineage

Viral myocarditis

p53 signaling pathway

Antigen processing and presentation

Arachidonic acid metabolism

NOD-like receptor signaling pathway

Systemic lupus erythematosus

Glutathione metabolism

RIG-I-like receptor signaling pathway

Neuroactive ligand-receptor interaction

Colorectal cancer

Prostate cancer

Bladder cancer

Wnt signaling pathway

Natural killer cell mediated cytotoxicity

Focal adhesion

Epithelial cell signaling in Helicobacter pylori infection

Regulation of autophagy

Arginine and proline metabolism

Metabolism of xenobiotics by cytochrome P450

Endocytosis

Alanine

Glycerolipid metabolism

aspartate and glutamate metabolism

Leukocyte transendothelial migration

Cyanoamino acid metabolism

Selenoamino acid metabolism

Taurine and hypotaurine metabolism

GnRH signaling pathway

MAPK signaling pathway

Cytokine-cytokine receptor interaction

Melanoma

Renal cell carcinoma

Cytosolic DNA-sensing pathway

Endometrial cancer

Non-small cell lung cancer

Pancreatic cancer

Protein export

Small cell lung cancer

Toll-like receptor signaling pathway

VEGF signaling pathway

B cell receptor signaling pathway

Notch signaling pathway

T cell receptor signaling pathway

Type I diabetes mellitus

Tryptophan metabolism

Cardiac muscle contraction

Oxidative phosphorylation

Hypertrophic cardiomyopathy (HCM)

Purine metabolism

Amino sugar and nucleotide sugar metabolism

Histidine metabolism

beta-Alanine metabolism

PPAR signaling pathway

Regulation of actin cytoskeleton

Allograft rejection

Autoimmune thyroid disease

Graft-versus-host disease

Hedgehog signaling pathway

Spliceosome

Neurotrophin signaling pathway

Other glycan degradation

Basal transcription factors

Folate biosynthesis

Glycosylphosphatidylinositol(GPI)-anchor biosynthesis

Cell cycle

Oocyte meiosis

Arrhythmogenic right ventricular cardiomyopathy (ARVC)

Neutrophils

Human

Human 293

Plasma

Luminex

96-well plate, Luminex

96-well plate

Neomycin

Puromycin

None

TA600150

TA600400

TA700155

TA700399

TA600053

TA600158

TA600163

TA600273

TA700159

TA700160

TA700273

TA700378

TA600052

TA600151, TA600152, TA600153, TA600154, TA600155

TA600153

TA600153, TA600155

TA600156

TA600157

TA600159, TA600160

TA600161, TA600162

TA600273, TA600274, TA600275, TA600276

TA600274

TA600378, TA600380

TA600379

TA600399, TA600401, TA600402

TA600424

TA600425

TA600426

TA600427

TA600428

TA600435

TA600436

TA600437

TA600438

TA600439

TA600568

TA700051, TA700053, TA700223

TA700052

TA700150, TA700151, TA700152, TA700153, TA700154

TA700155, TA700190

TA700155, TA700190, TA700191

TA700156

TA700157

TA700158, TA700163

TA700161, TA700162

TA700273, TA700274, TA700275

TA700273, TA700276

TA700379

TA700400, TA700401, TA700402

TA700424

TA700425

TA700426

TA700427

TA700428

TA700435

TA700436

TA700437

TA700438

TA700439

Carrier-free Antibodies

1

2

3

Yes

In Stock