Products

cDNA Clones

Antibodies

Proteins

Expression Plasmids

Primary Antibodies

Recombinant Proteins

CytoSections

Mutant ORF Clones

Mass Spec Standards

Mutant Proteins

Lentiviral Particles

Native Proteins

Over-expression Lysates

Mutant CytoSections

Human

Mouse

Humon

BRCA1

BTK

MLH1

P53

GLUCOKINASE

MENIN

BRCA2

TUBERIN

MSH2

RET

FGFR1

BETA CATENIN

EGFR

NOTCH3

ALPHA 1 ANTITRYPSIN

HER2

CREM

NF2

VEGFA

AMYLOID PRECURSOR PROTEIN

BDNF

DYSTROBREVIN ALPHA

PDE9A

AKT1

B RAF

CTNND1

G PROTEIN ALPHA S

EMA

CONSTITUTIVE ANDROSTANE RECEPTOR

NRG1

FGFR2

TAU

CD44

LKB1

WILMS TUMOR PROTEIN

B7-1

P16INK4A

GLUCOCORTICOID RECEPTOR

MSH6

CD31

HNF 4 ALPHA

ERG

CD33

CD4

P38

ESTROGEN RELATED RECEPTOR GAMMA

PD1

MU OPIOID RECEPTOR

FH

PI 3 KINASE CATALYTIC SUBUNIT ALPHA

CD20

MEK1

KRAS

TGIF

CD56

CD163

JNK1

APC11

CD36

CD45

CACNA1G

P63

ASPARTATE BETA HYDROXYLASE

CD117

KALLIKREIN 8

ALKALINE PHOSPHATASE

C-JUN

FGF1

IKAROS

NM23A

P73

BCL2

E CADHERIN

GBA

CAMK2B

CD147

CD46

CD63

ESTROGEN RECEPTOR 1

TPM1

CD19

GM CSF RECEPTOR ALPHA

PTEN

ATAXIN 3

AMACR

L1CAM

PRKAR1B

CACNA1C

MSF

PERIOSTIN

CD10

P21 RAS

AK3L1

FLT4

MBD1

EPOXIDE HYDROLASE

IRF3

MTOR

AURORA A

HMGA1

Human

Mouse

Rat

Monkey

Dog

Bovine

Human (Expected from sequence similarity: Mouse

Cow)

Pig

Human (Predicted: Mouse

Canine

Chicken

Rabbit

Bovine)

Dog)

Hamster

Primate

Porcine

Rat)

Gorilla

Pig)

Xenopus

Zebrafish

Bat

Rabbit)

Gibbon

Human (Predicted: Monkey)

Chimpanzee

Horse

Guinea Pig

Rhesus Monkey)

X. tropicalis)

Cow

Mouse (Expected from sequence similarity: Rat

Human (Predicted: Monkey

Human (Predicted: Rat

Rat (Expected from sequence similarity: Dog

Sheep

Mouse (Predicted: Rat

Equine

Xenopus)

Human (Expected from sequence similarity: Dog)

Human (Predicted: Mouse)

Human (Expected from sequence similarity: Dog

Mouse)

Rhesus Monkey

Horse)

Human (Predicted: Bovine

Feline

Drosophila

Chicken)

Human (Expected from sequence similarity: Rat

Human (Predicted: Bovine)

Human (Does not react with: Mouse)

Mouse (Expected from sequence similarity: Human

Macaque

Mammalian

Human (Expected from sequence similarity: Mouse)

Mouse (Predicted: Rat)

Hamster)

Mouse (Predicted: Bovine)

Human (Expected from sequence similarity: Cow)

Human (Predicted: Bat

Human (Predicted: Rhesus Monkey)

Monkey)

Rat (Expected from sequence similarity: Dog)

Rat (Expected from sequence similarity: Mouse

Orang-Utan

Pig (Expected from sequence similarity: Dog

Fish

Goat

Human (Predicted: Pig

Rat (Expected from sequence similarity: Human

Gibbon (Predicted: Monkey)

Human (Predicted: Rat)

Mouse (Predicted: Chicken

Human (Predicted: Dog

Mouse (Predicted: Rhesus Monkey)

X. tropicalis

Zebrafish)

Human (Predicted: Chicken

Human (Predicted: Pig)

Yeast

Canis

Human (Predicted: Chimpanzee)

Mouse (Predicted: Pig

Gibbon (Predicted: Monkey

Guinea Pig)

Mouse (Predicted: Bovine

All Species

Human (Expected from sequence similarity: Pig

Human (Predicted: Bat)

Human (Predicted: Dog)

Mouse (Expected from sequence similarity: Dog

Mouse (Expected from sequence similarity: Rat)

Rat (Expected from sequence similarity: Cow)

Rat (Predicted: Mouse

Frog

Gerbil

Human (Expected from sequence similarity: Rat)

Human (Predicted: Xenopus)

Opossum

Chimpanzee)

Expected from sequence similarity: Human

Horse (Predicted: Monkey

Horse (Predicted: Mouse

Human (Expected from sequence similarity: Pig)

Human (Predicted: Chicken)

Human (Predicted: Cow)

Human (Predicted: Hamster)

Human (Predicted: Rabbit)

Mouse (Predicted: Chimpanzee

Mouse (Predicted: Chimpanzee)

Mouse (Predicted: Dog

Mouse (Predicted: Monkey)

Birds

Gibbon (Predicted: Mouse

Hamster (Predicted: Monkey

Horse (Predicted: Bovine

Horse (Predicted: Monkey)

Human (Expected from sequence similarity: Feline

Human (Predicted: Chimpanzee

Insect

Orang-Utan (Predicted: Monkey)

Pig (Expected from sequence similarity: Rat

Rat (Expected from sequence similarity: Pig

Rat (Predicted: Bovine

African clawed frog

Amphibian

Avian

Baboon

C. elegans)

Clostridium botulinum

Ferret

Gibbon (Predicted: Bat

Gibbon (Predicted: Dog

Horse (Predicted: Bat

Horse (Predicted: Xenopus)

Human (Predicted: Rabbit

Human (Predicted: Xenopus

Human (Predicted: Zebrafish

Invertebrate

Mouse (Expected from sequence similarity: Dog)

Mouse (Predicted: Dog)

Mouse (Predicted: Monkey

Mouse (Predicted: Pig)

Murine

Orang-Utan (Predicted: Mouse

Pig (Expected from sequence similarity: Dog)

Plant

Quail

Rat (Expected from sequence similarity: Mouse)

Saccharomyces cerevisiae

Turkey

Algal

Bonito

Broad

Brugia malayi

C. elegans

C. elegans (Does not react with: Human

Chicken (weak)

Chlamydomonas

Coral

Cow (Expected from sequence similarity: Dog)

Cow (Expected from sequence similarity: Mouse

Dog (Expected from sequence similarity: Human

Dog (Expected from sequence similarity: Mouse

Dog (Expected from sequence similarity: Rat

Drosophila (weak)

Drosophila)

Escherichia coli

Eukaryote

Feline)

Fungi

Fungus

Gibbon (Predicted: Hamster)

Gibbon (Predicted: Mouse)

Golden Syrian Hamster

Guinea Pig (Predicted: Bat

Guinea Pig (Predicted: Bat)

Guinea Pig (Predicted: Hamster)

Guinea Pig (Predicted: Mouse

Hamster (Predicted: Bat

Hamster (Predicted: Dog

Hamster (Predicted: Mouse

Hamster (Predicted: Mouse)

Hamster (Predicted: Rabbit)

Hamster (Predicted: Rat

Helicobacter pylori

Horse (Predicted: Bat)

Horse (Predicted: Bovine)

Horse (Predicted: Dog

Horse (Predicted: Hamster)

Horse (Predicted: Rabbit

Horse (Predicted: Rat

Human (Predicted: Cow

Human (Predicted: Feline

Human (Predicted: Goat

Human (Predicted: Guinea Pig

Human (Predicted: Hamster

Human (Predicted: Horse

Human (Predicted: X. tropicalis)

Medaka fish)

Monkey (Predicted: Bat

Monkey (Predicted: Dog

Monkey (Predicted: Horse)

Monkey (Predicted: Rat

Mouse (Does not react with: Human)

Mouse (Does not react with: Rabbit)

Mouse (Does not react with: Rat)

Mouse (Expected from sequence similarity: Human)

Mouse (Predicted: Chicken)

Mouse (Predicted: Feline

Mouse (Predicted: Feline)

Mouse (Predicted: Rabbit

Mouse (Predicted: Rabbit)

Mouse (Predicted: Xenopus

Mouse (Predicted: Zebrafish

Mouse (Predicted: Zebrafish)

Mouse (weak)

Mustelid

Nicotiana

Orang-Utan (Predicted: Bat

Orang-Utan (Predicted: Bovine

Orang-Utan (Predicted: Hamster

Orang-Utan (Predicted: Horse)

Orang-Utan (Predicted: Monkey

Orang-Utan (Predicted: Zebrafish)

Pig (Expected from sequence similarity: Cow)

Pig (Expected from sequence similarity: Feline

Pig (Expected from sequence similarity: Human

Pig (Predicted: Bovine

Porcine (Does not react with: Mouse)

Predicted: Human

Primate (Does not react with: Mouse)

Primate)

Rabbit (Expected from sequence similarity: Pig

Rat (Does not react with: Human

Rat (Does not react with: Human)

Rat (Expected from sequence similarity: Cow

Rat (Predicted: Bovine)

Rat (Predicted: Chimpanzee)

Rat (Predicted: Monkey

Rat (Predicted: Mouse)

Rat (Predicted: Pig)

Rat (Predicted: Rabbit)

Salmonella Typhimurium

Sheep)

Spinach

Squirrel

Trichinella spiralis

Tuna

White Fly

Xenopus (weak)

Yeast (Does not react with: Xenopus)

WB

IHC

IF

FC

ELISA

IP

ICC/IF

LMNX

Simple Western

10k-ChIP

FN

PEP-ELISA

ChIP

Neutralize

Dot

ID

Immunoblotting

R

CyTOF-ready

SISCAPA

ICC

Block/Neutralize

Electron Microscopy

SDS-PAGE

E

EMSA

IA

Ie

LF

Labeling

Protein Array

RNAi

Sandwich ELISA

Mouse

Rabbit

Goat

Rat

Sheep

Chicken

Guinea Pig

Human

Hamster

HEK293T

HEK293

E. coli

Sf9

Insect

CHO

Baculovirus

Pichia

Plasmids

Particles

Myc-DDK

Tag Free

mGFP

C-Myc/DDK

TurboGFP

C-MYC/DDK

N-His

N-GST and C-His

C-DDK

C-His

C-DDK/His

DDK-His

C-6His

N-GST and C-HIS

His-tag

N-His-ABP (Albumin-Binding Protein)

C-HIS

C-Fc

C-8His

C-Human Fc

N-His-Trx

C-6×His

C-hFc

N-6His

C-Mouse Fc

His-Tag

C-Avi&6His

Tag-free

C-Mouse Fc and 6×His

C- 6×His

C-10His

N-HIS

C-Human Fc and 6×His

C-hFc&His

N-8His

N-His/DDK

C-Fc/His

C-HUMAN

N-GST

N-GST, C-His

N-His-PDCD1(Pro21-Val170)

N-Mouse Fc and C-6×His

C-6xHis/AVI

C-AVI-PLUS

C-DDK/10xHis

C-DDK/6xHis

C-FC

C-FLAG-Fc

C-HIS/DDK

C-Myc-DDK

N-6His-FLAG

N-AVI

N-Avi&6His

N-DDK

N-DDK/His

N-FLAG

N-FUSION

N-Fc-FLAG

N-Flag&C-His

N-GFP

N-GST(CKM) and N-His(CKB)

N-GST/C-His

N-His-GST

N-Human Fc

N-T7

N-TRX

N-TRXA

N-hFc

N-terminal His

Tag free

pCMV6-Entry

pCMV6-AC-GFP

pLenti-C-Myc-DDK-P2A-Puro

pLenti-C-mGFP-P2A-Puro

pLenti-C-Myc-DDK

pLenti-C-mGFP

pCMV6-XL5

pCMV6-XL4

pCMV6-AC

pCMV6-XL6

pCMV6-AC-Myc-DDK

PCMV6-Neo

pCMV6-XL5-DDK-His

pCMV6-AN-Myc-DDK

pRMT-tGFP, Promoter-less GFP Reporter Vector

KO Validated Antibodies

Unconjugated

Biotin

HRP

FITC

PE

APC

Peroxidase

PerCP

DyLight 488

TRITC

APC-Cy7

ATTO 655

Cy5

DyLight 550

DyLight 633

Magnetic Beads

PE-Cy5.5

PE-Cy7

Phospho-specific

IgG1

IgG

IgG2a

IgG2b

Ig

IgG1, kappa

IgG3

IgM

IgG2a, kappa

IgG2b, kappa

IgY

IgG2c

IgM, kappa

IgG3, kappa

IgG, kappa

IgG1, lambda

IgG1/IgK

Monoclonal

Polyclonal

No

Yes

Indirect

Sandwich

Direct

LentiORF

TrueORF

TrueMAB

TrueClone

MVPro

CytoSections

TrueORF Gold, TrueORF

UltraMAB

TrueRAB

SDS PAGE

Druggable Genome

Transmembrane

WB

Transcription Factors

Secreted Protein

Protein Kinase

ES Cell Differentiation/IPS

Protease

Stem cell - Pluripotency

GPCR

Phosphatase

Adult stem cells

Ion Channels: Other

Embryonic stem cells

Nuclear Hormone Receptor

Cancer stem cells

Ion Channels: Potassium

Induced pluripotent stem cells

P450

Stem cell relevant signaling - JAK/STAT signaling pathway

Ion Channels: Cys-loop Receptors

Stem cell relevant signaling - TGFb/BMP signaling pathway

Stem cell relevant signaling - DSL/Notch pathway

Ion Channels: Transient receptor potential

Stem cell relevant signaling - Wnt Signaling pathway

Ion Channels: Glutamate Receptors

Ion Channels: Calcium

Ion Channels: Sodium

Ion Channels: ATP Receptors

Ion Channels: Cyclic nucleotide gated

ELISA

SDS-PAGE

Bioactivity

the solution can be further dialyzed against PBS (phosphate buffered saline

10 mM Tris-HCl

2 mM dithiothreitol

Enzyme Activity

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g. Dulbecco's PBS).

ELISA.
Western Blot.

Flow Cyt

pH 7.4). For immunization purposes

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.

pH 7.4).
For immunization purposes

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

e.g. Dulbeccos PBS).

e.g. Dulbeccos PBS).
See References 2 and 3 for more details.

pH 7.4).
For immunization purposes

pH 7.4).
For Immunization purposes

Biological activity/ Working concentration: Optimum concentration for human umbilical vein endothelial cells (HUVEC) range from 50-200 μg/ml

ELISA.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoblotting.
Receptor-binding studies.

Western Blot.

0.1% (w/v) insulin

000-1/5

10 mM sodium phosphate

10mM Tris-HCl

1mM DTT

2 mM dithiothreitol

2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes

2mM dithiothreitol

63mM sodium phosphate

Biological activity/ Working concentration: The working concentration of ECGF for HUVEC is in the range of 25µg/ml to 100µg/ml. When adding Heparin (2.5mg per mg ECGF) an ECGF concentration of 12µg/ml (30µg/ml Heparin) is optimal. In this case 6mg ECGF are sufficient for 500 ml medium.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above)

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to Filaments is performed by dissolving in 6 M urea buffer (see above) at concentrations of approx. 0.5 mg/ml. Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

Can be used as standard in a Sandwich ELISA.

FACS

Protein standard in 1D and 2D SDS gel electrophoresis.
Immunoassay.
Antigen for immunization.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above)

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after vimentin is dissolved in 6 M urea buffer (see above)

Protocol: 1. Uehara Y.

Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris

Secreted

Suitable for Immunoblotting (Western or Dot blot)

and Fukazawa H. (1991) Methods Enzymol. 201: 370.
2. Fukazawa H.

and Nuccitelli R. (1992) J.Biol.Chem. 267:5608.

both dissolved in 9.5 M urea buffer (see above).
Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g.Dulbecco's PBS).

et al. (1991) Biochem. Pharmacol. 42: 1661.
3. Satoh T.

et al.(1992) J.Biol.Chem. 267: 2537.
4. Weiss R.

optimal concentration with heparin (30 μg/ml) is about 12 μg/ml.

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl

the solution can be further dialyzed against PBS (Phosphate Buffered Saline

000) as well as other antibody based fluorescent assays.

000)
Flow Cytometry/FACS (1/1

000-1/200

000-1/32

000-1/40

000.

000.
Immunohistochemistry: 1/1

000.
Protein G Peroxidase is a useful reagent in Western Blotting and ELISA experiments. Protein G Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary antibody and appropriate substrate (such as TMB-1000 or Femtomax-110).

000.
Western Blot: 1/500-1/2

000.
Western blot: 1/10

500.
Immunohistochemistry: 1/200-1/1

ELISA.
Western Blot.
Cell culture and/or animal studies.

ELISA.
Suitable for use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

ELISA.
Western Blot.

ELISA.
Western blotting.

ELISA: this protein is suitable for use as a Mouse IgE standard in ELISA assays.

ELISA.

Functional Assays.

Positive control for Western blot analysis

Positive control for Western blot analysis.
Standard for ELISA.

Adermann K. Role of the prosequence of guanylin. Protein Sci. 1999 Sep; 8(9): 1850-9.
4. Lauber T

Cell culture and/or animal studies.
ELISA.
Western Blot.

Cohen MB. Proguanylin secretion and the role of negative-feedback inhibition in a villous epithelial cell line. Am J Physiol Gastrointest Liver Physiol. 2002 Sep; 283(3): G695-702.
3. Schulz A

ELISA and Immunohistochemistry as well as other beta galactosidase-avidin/biotin based enzymatic assays. This product has been assayed against 1.0 µg of biotinylated IgG in a standard capture ELISA using OPNG as a substrate for 30 minutes at room temperature.
Recommended Dilutions:
ELISA: 1/8000-1/32000.
Western Blot: 1/500-1/2500.
Immunohistochemistry: 1/200-1/1000.

ELISA and immunohistochemistry as well as other glucose oxidase-antibody based enzymatic assays.
Recommended Dilutions:
ELISA: 1/8

ELISA.
Functional Assays.

ELISA.
Western blotting.

For use as a Control or Standard in Indirect trapping ELISA for quantitation of antigen in serum using a standard curve

For use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

Forssmann WG

Hawkins JA

Hidaka Y

Immunofluorescence Microscopy (1/1

Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-biotin-avidin based enzymatic assays.
Recommended Dilutions:
ELISA: 1/20

Marx UC

Marx UC. Native and recombinant proguanylin feature identical biophysical properties and are monomeric in solution. Biochemistry. 2002 Dec 10; 41(49): 14602-12.
2. Rudolph JA

Marx UC. Solution structure of human proguanylin: the role of a hormone prosequence. J Biol Chem. 2003 Jun 27; 278(26): 24118-24. Epub 2003 Apr 21.

Neudecker P

Nourse A

Rosch P

Schulz A

Shimonishi Y

Specific methodologies have not been tested using this product.

Suitable for Immunoblotting (Western or dot blot)

Western Blot.
ELISA.

Western Blot.
Protocol: 1. Lauber T

Western Blotting.

change growth medium against basal medium (EBM) in the early afternoon]
- change EGF against EBM (for HUVEC: EBM +1-2% FCS)
- incubate 24 h
- change medium again and add factors (growth factors

etc.)
- incubate for 18 h
- add 10 µl 3H-Thymidine solution [0.025 mCi/ml] per well (=0.25 µCi)
- incubate another 6h at 37°C
- Washing steps: (250 µl/well)
PBS 1x
MeOH 2x 5 min
TCA 2x 10 min
H2O 1x
- lyse cells in 250 µl 0.3M NaOH per well
- transfer 2.5 ml ECO Plus into the appropriate scintillation vials
- transfer cell lysats into the scintillation vials
- count by liquid scintillation (ß-counter; Beckmann Instruments)

for immunoprecipitation and for Wwestern blotting.

inhibitors

optimal concentration with heparin (50 μg/ml) is about 12 μg/ml.
Protocol: Thymidine Incorporation with HUVEC
- plate cells with a density at 5-7 x 10e3 cells/well in growth medium (EGM)
- incubate cells over night [if urgent

plate cells in the morning