Products

cDNA Clones

Proteins

Expression Plasmids

Recombinant Proteins

CytoSections

Mutant ORF Clones

Mass Spec Standards

Human

Humon

BDNF

EGFR

CD44

PAX3

HGF

ERBB 3

OCT4

APOLIPOPROTEIN E

CD14

PAX6

MAP2

BMP4

CD4

GATA2

CCN4

EGF

CD8A

CD97

LEF1

GATA3

PAX7

TAL1

CD34

GLI1

CD117

GDF 9

LIF

KLF4

KGF

CD72

FGF19

FGF21

BMP2

GATA1

HNF1 ALPHA

WNT5A

CD9

DKK1

DLL1

HES1

IGFBP1

NEUROD1

SOX10

BMP6

HDAC1

IGFBP2

SMURF 2

SOX1

SOX2

SOX9

FGF10

FGF4

BMP7

GLI2

WNT6

CDX2

GDF 5

REX1

SFRP4

SFRP5

WNT1

WNT2

WNT3A

BMP5

BMP8B

DKK2

DKK4

GDF3

PDGFC

SFRP1

WNT7B

BMP15

NOTCH1

GDF7

GLI3

IHC

HEK293T

E. coli

HEK293

Sf9

Insect

CHO

Plasmids

Myc-DDK

mGFP

Tag Free

C-Myc/DDK

TurboGFP

C-MYC/DDK

N-His

C-His

C-Fc

DDK-His

C-DDK/His

C-6His

C-DDK

N-GST and C-HIS

N-Trx&His

N-GST and C-His

C-6His-Avi

C-Human Fc

C- 6×His

C-6×His

C-Avi-6His

C-hFc

C-mFc

N-His-ABP (Albumin-Binding Protein)

N-T7

Tag-free

pCMV6-Entry

pCMV6-AC-GFP

pLenti-C-Myc-DDK-P2A-Puro

pLenti-C-mGFP-P2A-Puro

pLenti-C-Myc-DDK

pLenti-C-mGFP

pCMV6-XL5

pCMV6-XL4

pCMV6-AC

pCMV6-XL5-DDK-His

pCMV6-XL6

PCMV6-Neo

No

Yes

LentiORF

TrueORF

TrueClone

MVPro

CytoSections

TrueORF Gold, TrueORF

SDS PAGE

Druggable Genome

Transmembrane

WB

Transcription Factors

Secreted Protein

Protein Kinase

Protease

ES Cell Differentiation/IPS

Stem cell - Pluripotency

GPCR

Phosphatase

Ion Channels: Other

Adult stem cells

Nuclear Hormone Receptor

Embryonic stem cells

Ion Channels: Potassium

Cancer stem cells

P450

Induced pluripotent stem cells

Ion Channels: Cys-loop Receptors

Stem cell relevant signaling - JAK/STAT signaling pathway

Ion Channels: Transient receptor potential

Stem cell relevant signaling - TGFb/BMP signaling pathway

Stem cell relevant signaling - Wnt Signaling pathway

Stem cell relevant signaling - DSL/Notch pathway

Ion Channels: Glutamate Receptors

Ion Channels: Calcium

Ion Channels: Sodium

Ion Channels: ATP Receptors

Ion Channels: Cyclic nucleotide gated

ELISA

SDS-PAGE

Bioactivity

10 mM Tris-HCl

the solution can be further dialyzed against PBS (phosphate buffered saline

Flow Cyt

2 mM dithiothreitol

ELISA.
Western Blot.

e.g. Dulbecco's PBS).

Enzyme Activity

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

pH 7.4). For immunization purposes

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

pH 7.4).
For immunization purposes

pH 7.4).
For immunization purposes

Western Blot.

0.1% (w/v) insulin

10mM Tris-HCl

1mM DTT

2 mM dithiothreitol

2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes

2mM dithiothreitol

63mM sodium phosphate

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above)

Can be used as standard in a Sandwich ELISA.

FACS

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above)

Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris

Secreted

both dissolved in 9.5 M urea buffer (see above).
Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g. Dulbeccos PBS).

e.g.Dulbecco's PBS).

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl

the solution can be further dialyzed against PBS (Phosphate Buffered Saline

ELISA.
Suitable for use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

ELISA.
Western Blot.

ELISA.
Western blotting.

ELISA.

Positive control for Western blot analysis

Positive control for Western blot analysis.
Standard for ELISA.

Adermann K. Role of the prosequence of guanylin. Protein Sci. 1999 Sep; 8(9): 1850-9.
4. Lauber T

Cell culture and/or animal studies.
ELISA.
Western Blot.

Cohen MB. Proguanylin secretion and the role of negative-feedback inhibition in a villous epithelial cell line. Am J Physiol Gastrointest Liver Physiol. 2002 Sep; 283(3): G695-702.
3. Schulz A

For use as a Control or Standard in Indirect trapping ELISA for quantitation of antigen in serum using a standard curve

For use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

Forssmann WG

Hawkins JA

Hidaka Y

Marx UC

Marx UC. Native and recombinant proguanylin feature identical biophysical properties and are monomeric in solution. Biochemistry. 2002 Dec 10; 41(49): 14602-12.
2. Rudolph JA

Marx UC. Solution structure of human proguanylin: the role of a hormone prosequence. J Biol Chem. 2003 Jun 27; 278(26): 24118-24. Epub 2003 Apr 21.

Neudecker P

Nourse A

Rosch P

Schulz A

Shimonishi Y

Specific methodologies have not been tested using this product.

Western Blot.
Protocol: 1. Lauber T

Western Blotting.

for immunoprecipitation and for Wwestern blotting.

Pathways in cancer

MAPK signaling pathway

Cytokine-cytokine receptor interaction

Melanoma

Regulation of actin cytoskeleton

Hematopoietic cell lineage

Endocytosis

Hedgehog signaling pathway

Basal cell carcinoma

Focal adhesion

ErbB signaling pathway

Prostate cancer

Wnt signaling pathway

Endometrial cancer

Huntington's disease

Neurotrophin signaling pathway

Melanogenesis

Calcium signaling pathway

Gap junction

Bladder cancer

Glioma

Non-small cell lung cancer

Pancreatic cancer

Adherens junction

Colorectal cancer

TGF-beta signaling pathway

Dorso-ventral axis formation

Cell adhesion molecules (CAMs)

Epithelial cell signaling in Helicobacter pylori infection

GnRH signaling pathway

Maturity onset diabetes of the young

Antigen processing and presentation

Acute myeloid leukemia

ECM-receptor interaction

Primary immunodeficiency

T cell receptor signaling pathway

Notch signaling pathway

Renal cell carcinoma

Alzheimer's disease

Pathogenic Escherichia coli infection

Toll-like receptor signaling pathway

Arrhythmogenic right ventricular cardiomyopathy (ARVC)

Thyroid cancer

Jak-STAT signaling pathway

B cell receptor signaling pathway

Allograft rejection

Autoimmune thyroid disease

Cell cycle

Chronic myeloid leukemia

Graft-versus-host disease

Type I diabetes mellitus

Ubiquitin mediated proteolysis

Viral myocarditis

Prion diseases

Human

E. coli

Human 293

Neomycin

None

Puromycin

1

2

Yes

In Stock