Products

Antibodies

cDNA Clones

Proteins

Primary Antibodies

Expression Plasmids

Recombinant Proteins

CytoSections

Mutant ORF Clones

Mass Spec Standards

Human

Humon

EGFR

CD44

GLI1

BDNF

CD4

CD117

ERBB 3

FGF21

APOLIPOPROTEIN E

CD34

CD14

CD8A

GATA3

HGF

MAP2

HES1

OCT4

PAX3

HDAC1

EGF

CDX2

BMP4

IGFBP1

TAL1

PAX6

LEF1

PAX7

SOX9

BMP6

GLI2

REX1

SOX2

NOTCH1

CCN4

CD97

GATA2

KLF4

CD9

LIF

GDF 9

GATA1

BMP2

HNF1 ALPHA

KGF

CD72

FGF4

IGFBP2

FGF19

WNT5A

BMP7

DKK1

DLL1

NEUROD1

WNT1

WNT3A

SOX1

FGF10

SFRP1

SMURF 2

BMP8B

SOX10

WNT2

DKK4

WNT6

DKK2

SFRP4

SFRP5

BMP15

BMP5

GDF 5

PDGFC

WNT7B

GDF3

GLI3

GDF7

Human

Mouse

Rat

Dog

Monkey

Human (Expected from sequence similarity: Mouse

Bovine

Cow)

Primate

Canine

Pig

Porcine

Chicken

Human (Predicted: Mouse

Feline

Rabbit

Bat

Dog)

Pig)

Rat)

Sheep

Chimpanzee

Guinea Pig

Hamster

Human (Predicted: Mouse)

Macaque

Rat (Expected from sequence similarity: Dog)

Canis

Cow

Gibbon

Goat

Gorilla

Guinea Pig)

Hamster)

Horse

Horse (Predicted: Monkey

Human (Expected from sequence similarity: Dog

Human (Expected from sequence similarity: Dog)

Human (Expected from sequence similarity: Rat

Human (Predicted: Bovine

Human (Predicted: Chicken)

Human (Predicted: Monkey

Human (Predicted: Monkey)

Mouse (Does not react with: Rabbit)

Mouse (Does not react with: Rat)

Mouse (Expected from sequence similarity: Rat

Mouse (Predicted: Rat)

Opossum

Pig (Expected from sequence similarity: Rat

Quail

Rabbit)

Rat (Expected from sequence similarity: Human)

X. tropicalis)

Xenopus

Xenopus)

Zebrafish

WB

IHC

FC

IF

ELISA

ICC/IF

LMNX

IP

10k-ChIP

FN

Dot

Simple Western

PEP-ELISA

ChIP

CyTOF-ready

Immunoblotting

Mouse

Rabbit

Goat

Chicken

Rat

HEK293T

E. coli

HEK293

Sf9

Insect

CHO

Plasmids

Myc-DDK

mGFP

Tag Free

C-Myc/DDK

TurboGFP

C-MYC/DDK

N-His

C-His

C-Fc

DDK-His

C-DDK/His

C-6His

C-DDK

N-GST and C-HIS

N-Trx&His

N-GST and C-His

C-6His-Avi

C-Human Fc

C- 6×His

C-6×His

C-Avi-6His

C-hFc

C-mFc

N-His-ABP (Albumin-Binding Protein)

N-T7

Tag-free

pCMV6-Entry

pCMV6-AC-GFP

pLenti-C-Myc-DDK-P2A-Puro

pLenti-C-mGFP-P2A-Puro

pLenti-C-Myc-DDK

pLenti-C-mGFP

pCMV6-XL5

pCMV6-XL4

pCMV6-AC

pCMV6-XL5-DDK-His

pCMV6-XL6

PCMV6-Neo

KO Validated Antibodies

Unconjugated

Biotin

HRP

FITC

PE

APC

PerCP

APC-Cy7

Cy5

DyLight 488

PE-Cy5.5

PE-Cy7

Phospho-specific

IgG1

IgG

IgG2b

IgG2a

Ig

IgG1, kappa

IgG2c

IgY

IgG2b, kappa

IgM

IgM, kappa

Monoclonal

Polyclonal

No

Yes

Indirect

Sandwich

Direct

TrueMAB

LentiORF

TrueORF

TrueClone

MVPro

CytoSections

TrueORF Gold, TrueORF

UltraMAB

TrueRAB

SDS PAGE

Druggable Genome

Transmembrane

Transcription Factors

WB

Secreted Protein

Protein Kinase

ES Cell Differentiation/IPS

Protease

Stem cell - Pluripotency

GPCR

Phosphatase

Adult stem cells

Ion Channels: Other

Nuclear Hormone Receptor

Embryonic stem cells

Cancer stem cells

Induced pluripotent stem cells

Ion Channels: Potassium

P450

Stem cell relevant signaling - JAK/STAT signaling pathway

Stem cell relevant signaling - DSL/Notch pathway

Ion Channels: Cys-loop Receptors

Stem cell relevant signaling - TGFb/BMP signaling pathway

Ion Channels: Transient receptor potential

Stem cell relevant signaling - Wnt Signaling pathway

Ion Channels: Glutamate Receptors

Ion Channels: Calcium

Ion Channels: Sodium

Ion Channels: ATP Receptors

Ion Channels: Cyclic nucleotide gated

ELISA

SDS-PAGE

Bioactivity

the solution can be further dialyzed against PBS (phosphate buffered saline

10 mM Tris-HCl

2 mM dithiothreitol

Enzyme Activity

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g. Dulbecco's PBS).

ELISA.
Western Blot.

Flow Cyt

pH 7.4). For immunization purposes

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.

pH 7.4).
For immunization purposes

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

e.g. Dulbeccos PBS).

e.g. Dulbeccos PBS).
See References 2 and 3 for more details.

pH 7.4).
For immunization purposes

pH 7.4).
For Immunization purposes

Biological activity/ Working concentration: Optimum concentration for human umbilical vein endothelial cells (HUVEC) range from 50-200 μg/ml

ELISA.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoblotting.
Receptor-binding studies.

Western Blot.

0.1% (w/v) insulin

000-1/5

10 mM sodium phosphate

10mM Tris-HCl

1mM DTT

2 mM dithiothreitol

2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes

2mM dithiothreitol

63mM sodium phosphate

Biological activity/ Working concentration: The working concentration of ECGF for HUVEC is in the range of 25µg/ml to 100µg/ml. When adding Heparin (2.5mg per mg ECGF) an ECGF concentration of 12µg/ml (30µg/ml Heparin) is optimal. In this case 6mg ECGF are sufficient for 500 ml medium.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above)

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to Filaments is performed by dissolving in 6 M urea buffer (see above) at concentrations of approx. 0.5 mg/ml. Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

Can be used as standard in a Sandwich ELISA.

FACS

Protein standard in 1D and 2D SDS gel electrophoresis.
Immunoassay.
Antigen for immunization.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above)

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after vimentin is dissolved in 6 M urea buffer (see above)

Protocol: 1. Uehara Y.

Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris

Secreted

Suitable for Immunoblotting (Western or Dot blot)

and Fukazawa H. (1991) Methods Enzymol. 201: 370.
2. Fukazawa H.

and Nuccitelli R. (1992) J.Biol.Chem. 267:5608.

both dissolved in 9.5 M urea buffer (see above).
Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g.Dulbecco's PBS).

et al. (1991) Biochem. Pharmacol. 42: 1661.
3. Satoh T.

et al.(1992) J.Biol.Chem. 267: 2537.
4. Weiss R.

optimal concentration with heparin (30 μg/ml) is about 12 μg/ml.

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl

the solution can be further dialyzed against PBS (Phosphate Buffered Saline

000) as well as other antibody based fluorescent assays.

000)
Flow Cytometry/FACS (1/1

000-1/200

000-1/32

000-1/40

000.

000.
Immunohistochemistry: 1/1

000.
Protein G Peroxidase is a useful reagent in Western Blotting and ELISA experiments. Protein G Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary antibody and appropriate substrate (such as TMB-1000 or Femtomax-110).

000.
Western Blot: 1/500-1/2

000.
Western blot: 1/10

500.
Immunohistochemistry: 1/200-1/1

ELISA.
Western Blot.
Cell culture and/or animal studies.

ELISA.
Suitable for use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

ELISA.
Western Blot.

ELISA.
Western blotting.

ELISA: this protein is suitable for use as a Mouse IgE standard in ELISA assays.

ELISA.

Functional Assays.

Positive control for Western blot analysis

Positive control for Western blot analysis.
Standard for ELISA.

Adermann K. Role of the prosequence of guanylin. Protein Sci. 1999 Sep; 8(9): 1850-9.
4. Lauber T

Cell culture and/or animal studies.
ELISA.
Western Blot.

Cohen MB. Proguanylin secretion and the role of negative-feedback inhibition in a villous epithelial cell line. Am J Physiol Gastrointest Liver Physiol. 2002 Sep; 283(3): G695-702.
3. Schulz A

ELISA and Immunohistochemistry as well as other beta galactosidase-avidin/biotin based enzymatic assays. This product has been assayed against 1.0 µg of biotinylated IgG in a standard capture ELISA using OPNG as a substrate for 30 minutes at room temperature.
Recommended Dilutions:
ELISA: 1/8000-1/32000.
Western Blot: 1/500-1/2500.
Immunohistochemistry: 1/200-1/1000.

ELISA and immunohistochemistry as well as other glucose oxidase-antibody based enzymatic assays.
Recommended Dilutions:
ELISA: 1/8

ELISA.
Functional Assays.

ELISA.
Western blotting.

For use as a Control or Standard in Indirect trapping ELISA for quantitation of antigen in serum using a standard curve

For use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

Forssmann WG

Hawkins JA

Hidaka Y

Immunofluorescence Microscopy (1/1

Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-biotin-avidin based enzymatic assays.
Recommended Dilutions:
ELISA: 1/20

Marx UC

Marx UC. Native and recombinant proguanylin feature identical biophysical properties and are monomeric in solution. Biochemistry. 2002 Dec 10; 41(49): 14602-12.
2. Rudolph JA

Marx UC. Solution structure of human proguanylin: the role of a hormone prosequence. J Biol Chem. 2003 Jun 27; 278(26): 24118-24. Epub 2003 Apr 21.

Neudecker P

Nourse A

Rosch P

Schulz A

Shimonishi Y

Specific methodologies have not been tested using this product.

Suitable for Immunoblotting (Western or dot blot)

Western Blot.
ELISA.

Western Blot.
Protocol: 1. Lauber T

Western Blotting.

change growth medium against basal medium (EBM) in the early afternoon]
- change EGF against EBM (for HUVEC: EBM +1-2% FCS)
- incubate 24 h
- change medium again and add factors (growth factors

etc.)
- incubate for 18 h
- add 10 µl 3H-Thymidine solution [0.025 mCi/ml] per well (=0.25 µCi)
- incubate another 6h at 37°C
- Washing steps: (250 µl/well)
PBS 1x
MeOH 2x 5 min
TCA 2x 10 min
H2O 1x
- lyse cells in 250 µl 0.3M NaOH per well
- transfer 2.5 ml ECO Plus into the appropriate scintillation vials
- transfer cell lysats into the scintillation vials
- count by liquid scintillation (ß-counter; Beckmann Instruments)

for immunoprecipitation and for Wwestern blotting.

inhibitors

optimal concentration with heparin (50 μg/ml) is about 12 μg/ml.
Protocol: Thymidine Incorporation with HUVEC
- plate cells with a density at 5-7 x 10e3 cells/well in growth medium (EGM)
- incubate cells over night [if urgent

plate cells in the morning

Pathways in cancer

Hematopoietic cell lineage

MAPK signaling pathway

Regulation of actin cytoskeleton

Cytokine-cytokine receptor interaction

Endocytosis

Melanoma

Hedgehog signaling pathway

ErbB signaling pathway

Basal cell carcinoma

Focal adhesion

Calcium signaling pathway

Prostate cancer

Endometrial cancer

Gap junction

Cell adhesion molecules (CAMs)

Bladder cancer

Glioma

Non-small cell lung cancer

Pancreatic cancer

Adherens junction

Colorectal cancer

Dorso-ventral axis formation

Epithelial cell signaling in Helicobacter pylori infection

GnRH signaling pathway

Melanogenesis

Antigen processing and presentation

Primary immunodeficiency

T cell receptor signaling pathway

Huntington's disease

Wnt signaling pathway

ECM-receptor interaction

TGF-beta signaling pathway

Notch signaling pathway

Acute myeloid leukemia

Neurotrophin signaling pathway

Maturity onset diabetes of the young

Alzheimer's disease

Pathogenic Escherichia coli infection

Toll-like receptor signaling pathway

Renal cell carcinoma

Cell cycle

Chronic myeloid leukemia

Arrhythmogenic right ventricular cardiomyopathy (ARVC)

Thyroid cancer

Prion diseases

Jak-STAT signaling pathway

B cell receptor signaling pathway

Allograft rejection

Autoimmune thyroid disease

Graft-versus-host disease

Type I diabetes mellitus

Ubiquitin mediated proteolysis

Viral myocarditis

Human

E. coli

Human 293

96-well plate

Luminex

96-well plate, Luminex

Neomycin

None

Puromycin

TA600277

TA600279

TA700278

TA700279

TA700469

TA700484

TA600053

TA600278, TA600280

TA600281, TA600282

TA600469, TA600472

TA600470

TA600471

TA600473

TA600474

TA600482

TA600482, TA600484

TA600483, TA600485, TA600484

TA600541

TA600542

TA600543

TA600544

TA700277, TA700298, TA700300

TA700297, TA700299, TA700301

TA700470

TA700471

TA700472

TA700473

TA700482, TA700483

TA700483, TA700484

TA700542

TA700543

TA700544

TA700545

Carrier-free Antibodies

1

2

3

Yes

In Stock