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Proteins

Recombinant Proteins

Human

Mouse

Rat

Escherichia coli

Blomia tropicalis

SARS-CoV-2

Dermatophagoides pteronyssinus

Streptococcus Pyogenes

Staphylococcus aureus

Orf Virus

Bovine

Dermatophagoides farinae

Rabbit

Streptococcus sp.

Alternaria alternata

Betula pendula

E.coli

Enterobacteria phage T4

Escherichia phage T7

HIV

Hepatitis B Virus

Humulus japonicus

Lachnospiraceae bacterium ND2006

Lepidoglyphus destructor

Leptotrichia wadei

Macaca fascicularis

Mucor hiemalis

Mus musculus

Saccharomyces cerevisiae

Serratia marcescens

Staphylococcus aureus subsp.

Streptomyces avidinii

Streptomyces plicatus

Tobacco etch virus

Vaccinia virus

VEGFA

TNF ALPHA

FGF2

IL6

PTPRN

FGF1

NFYA

BDNF

EGF

EOTAXIN 3

IGF1

IL4

SDF1

ALPHA SYNUCLEIN

BMP2

FSTL1

HIP2

IL22

IL33

IL8

PDGF AA

SUMO 3

BNP

CSF2

FABP4

FERRITIN HEAVY CHAIN

IL1B

IL3

LIF

TRAIL

BLNK

CNTF

GDNF

GLUTAMINE SYNTHETASE

IL1 BETA

MAP1LC3A

NEUROTROPHIN 3

NHP2

TGIF

TNF

TRIM72

ADIPOQ

AMPHIREGULIN

ANNEXIN VI

APE1

APOLIPOPROTEIN E

ASB13

ASF1A

BETA ARRESTIN 1

BID

BIRC5

CALCITONIN

CASPASE 10

CSPS

CXCL14

EIF1A

EIF4EBP1

FGF21

FIBRONECTIN

GABARAPL1

GADD45B

GLUTATHIONE TRANSFERASE ZETA 1

HAO1

IL15

IL17F

IL1RA

IL7

INTERFERON GAMMA

KGF

KLF6

KPNA2

LACTB2

LEPTIN

LSM4

LYSOPHOSPHOLIPASE 1

MAX

MGMT

MYOZENIN 2

NCK

NEDD8

NEURITIN

NGAL

ONCOSTATIN M

PARATHYROID HORMONE

PDCD10

PDGF BETA

PDGFA

PEROXIREDOXIN 4

PGP9.5

PIN4

PKIB

SENP2

SUMO 1

SUPEROXIDE DISMUTASE 1

SYNTAXIN 6

TRYPTOPHANYL TRNA SYNTHETASE

TXN

UBE2G2

UPB1

YY1

HEK293T

HEK293

E. coli

CHO

Sf9

Insect

Baculovirus

Pichia

Pichia pastoris

Mouse

Other

S. cerevisiae

Sf9 insect

H. vulgare

Human

Yeast

N-His

Tag Free

C-His

N-GST and C-His

N-GST and C-HIS

N-GST

N-His-ABP (Albumin-Binding Protein)

His-tag

N-His&C-His

N-T7&C-His

N-Trx&His

N-His-Trx

N-GST, C-His

N-HIS

N-His&SUMO

Tag-free

N-6His

N-His PDCD1(Pro21-Val170)

N-GST&His

N-His&T7

N-His-GST

N-Trx

C-Avi&6His

C-HIS

His-tagged, myc-tagged

N-Avi

Myc-DDK

N-His-PDCD1(Pro21-Val170)

N-terminal His

N-terminal His-PDCD1(Pro21-Val170) tag

C-DDK

C-DDK-His

C-HIS/C

C-Myc/DDK

DDK-tag

His tag

N-AVI

N-DDK

N-FUSION

N-GFP

N-GST(CKM) and N-His(CKB)

N-HIS and C-AVIPLUS

N-PROTEIN

N-T7

N-TRX

N-TRXA

N-Trx-His

N-terminal His-tag

Druggable Genome

Secreted Protein

Transcription Factors

Transmembrane

ES Cell Differentiation/IPS

Protein Kinase

Stem cell - Pluripotency

Protease

Adult stem cells

Embryonic stem cells

Phosphatase

Induced pluripotent stem cells

Ion Channels: Other

Cancer stem cells

Stem cell relevant signaling - TGFb/BMP signaling pathway

Stem cell relevant signaling - JAK/STAT signaling pathway

Stem cell relevant signaling - DSL/Notch pathway

Stem cell relevant signaling - Wnt Signaling pathway

10 mM Tris-HCl

GPCR

Nuclear Hormone Receptor

the solution can be further dialyzed against PBS (phosphate buffered saline

2 mM dithiothreitol

ELISA.
Western Blot.

e.g. Dulbecco's PBS).

Ion Channels: Potassium

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

P450

pH 7.4). For immunization purposes

Ion Channels: Transient receptor potential

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

pH 7.4).
For immunization purposes

pH 7.4).
For immunization purposes

Ion Channels: Cys-loop Receptors

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoblotting.
Receptor-binding studies.

Western Blot.

0.1% (w/v) insulin

10mM Tris-HCl

1mM DTT

2 mM dithiothreitol

2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes

2mM dithiothreitol

63mM sodium phosphate

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above)

Can be used as standard in a Sandwich ELISA.

Ion Channels: Sodium

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above)

Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris

both dissolved in 9.5 M urea buffer (see above).
Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g. Dulbeccos PBS).

e.g.Dulbecco's PBS).

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl

the solution can be further dialyzed against PBS (Phosphate Buffered Saline

ELISA.
Western blotting.

Positive control for Western blot analysis.
Standard for ELISA.

Adermann K. Role of the prosequence of guanylin. Protein Sci. 1999 Sep; 8(9): 1850-9.
4. Lauber T

Cohen MB. Proguanylin secretion and the role of negative-feedback inhibition in a villous epithelial cell line. Am J Physiol Gastrointest Liver Physiol. 2002 Sep; 283(3): G695-702.
3. Schulz A

ELISA

ELISA.
Western blotting.

Forssmann WG

Hawkins JA

Hidaka Y

Ion Channels: ATP Receptors

Marx UC

Marx UC. Native and recombinant proguanylin feature identical biophysical properties and are monomeric in solution. Biochemistry. 2002 Dec 10; 41(49): 14602-12.
2. Rudolph JA

Marx UC. Solution structure of human proguanylin: the role of a hormone prosequence. J Biol Chem. 2003 Jun 27; 278(26): 24118-24. Epub 2003 Apr 21.

Neudecker P

Nourse A

Rosch P

Schulz A

Shimonishi Y

Western Blot.
Protocol: 1. Lauber T

Western Blotting.

Metabolic pathways

Cytokine-cytokine receptor interaction

Pathways in cancer

MAPK signaling pathway

Jak-STAT signaling pathway

Chemokine signaling pathway

Hematopoietic cell lineage

Regulation of actin cytoskeleton

Focal adhesion

Melanoma

Apoptosis

Natural killer cell mediated cytotoxicity

Toll-like receptor signaling pathway

T cell receptor signaling pathway

NOD-like receptor signaling pathway

Ubiquitin mediated proteolysis

Alzheimer's disease

Prostate cancer

Systemic lupus erythematosus

Fc epsilon RI signaling pathway

Cell cycle

TGF-beta signaling pathway

Neurotrophin signaling pathway

Huntington's disease

Type I diabetes mellitus

Wnt signaling pathway

ErbB signaling pathway

Hypertrophic cardiomyopathy (HCM)

Parkinson's disease

p53 signaling pathway

Amyotrophic lateral sclerosis (ALS)

Glioma

RIG-I-like receptor signaling pathway

Graft-versus-host disease

Cytosolic DNA-sensing pathway

Oocyte meiosis

Renal cell carcinoma

Acute myeloid leukemia

Dilated cardiomyopathy

Insulin signaling pathway

Pancreatic cancer

PPAR signaling pathway

Adipocytokine signaling pathway

Prion diseases

Asthma

Small cell lung cancer

Melanogenesis

Pyrimidine metabolism

Colorectal cancer

Gap junction

Chronic myeloid leukemia

mTOR signaling pathway

B cell receptor signaling pathway

Endocytosis

Bladder cancer

Allograft rejection

Hedgehog signaling pathway

Purine metabolism

Ribosome

Nitrogen metabolism

Glycolysis / Gluconeogenesis

Leukocyte transendothelial migration

Progesterone-mediated oocyte maturation

Type II diabetes mellitus

Arginine and proline metabolism

Basal cell carcinoma

Spliceosome

Axon guidance

Antigen processing and presentation

VEGF signaling pathway

Calcium signaling pathway

Epithelial cell signaling in Helicobacter pylori infection

Regulation of autophagy

Adherens junction

Vascular smooth muscle contraction

Endometrial cancer

GnRH signaling pathway

Long-term potentiation

Amino sugar and nucleotide sugar metabolism

Autoimmune thyroid disease

Oxidative phosphorylation

Fructose and mannose metabolism

Base excision repair

Complement and coagulation cascades

Drug metabolism - other enzymes

Glutathione metabolism

Neuroactive ligand-receptor interaction

Porphyrin and chlorophyll metabolism

Cell adhesion molecules (CAMs)

ECM-receptor interaction

Non-small cell lung cancer

Long-term depression

Metabolism of xenobiotics by cytochrome P450

Tryptophan metabolism

Fc gamma R-mediated phagocytosis

Primary immunodeficiency

SNARE interactions in vesicular transport

Glycerophospholipid metabolism

Sulfur metabolism

Cardiac muscle contraction

Glycine

Pyruvate metabolism

RNA degradation

Tyrosine metabolism

serine and threonine metabolism

Cysteine and methionine metabolism

Pathogenic Escherichia coli infection

Inositol phosphate metabolism

Phenylalanine metabolism

Thyroid cancer

Tight junction

ABC transporters

Alanine

Arrhythmogenic right ventricular cardiomyopathy (ARVC)

Terpenoid backbone biosynthesis

Viral myocarditis

aspartate and glutamate metabolism

Glyoxylate and dicarboxylate metabolism

Phosphatidylinositol signaling system

Propanoate metabolism

Retinol metabolism

Aminoacyl-tRNA biosynthesis

Androgen and estrogen metabolism

Citrate cycle (TCA cycle)

Dorso-ventral axis formation

Drug metabolism - cytochrome P450

Arachidonic acid metabolism

Basal transcription factors

Fatty acid metabolism

Mismatch repair

Notch signaling pathway

Nucleotide excision repair

Olfactory transduction

Pantothenate and CoA biosynthesis

Valine

beta-Alanine metabolism

Ether lipid metabolism

Galactose metabolism

Homologous recombination

Lysine degradation

Lysosome

Maturity onset diabetes of the young

Methane metabolism

Nicotinate and nicotinamide metabolism

Pentose and glucuronate interconversions

Pentose phosphate pathway

Proteasome

leucine and isoleucine degradation

DNA replication

One carbon pool by folate

Renin-angiotensin system

Selenoamino acid metabolism

Ubiquinone and other terpenoid-quinone biosynthesis

Butanoate metabolism

N-Glycan biosynthesis

Riboflavin metabolism

Steroid biosynthesis

Vibrio cholerae infection

C21-Steroid hormone metabolism

Glycosphingolipid biosynthesis - lacto and neolacto series

Heparan sulfate biosynthesis

Primary bile acid biosynthesis

Sphingolipid metabolism

Synthesis and degradation of ketone bodies

Chondroitin sulfate biosynthesis

D-Arginine and D-ornithine metabolism

Histidine metabolism

Keratan sulfate biosynthesis

Linoleic acid metabolism

Non-homologous end-joining

O-Glycan biosynthesis

Starch and sucrose metabolism

Taste transduction

Taurine and hypotaurine metabolism

alpha-Linolenic acid metabolism

Circadian rhythm - mammal

Folate biosynthesis

RNA polymerase

Caffeine metabolism

D-Glutamine and D-glutamate metabolism

Glycosaminoglycan degradation

Glycosylphosphatidylinositol(GPI)-anchor biosynthesis

Limonene and pinene degradation

Other glycan degradation

Phenylalanine

leucine and isoleucine biosynthesis

tyrosine and tryptophan biosynthesis

Ascorbate and aldarate metabolism

Biosynthesis of unsaturated fatty acids

Cyanoamino acid metabolism

Fatty acid elongation in mitochondria

Glycerolipid metabolism

Glycosphingolipid biosynthesis - ganglio series

Lipoic acid metabolism

Protein export

1

2

3

Yes

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