活动过滤器
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Species
- Human (3103)
- Mouse (55)
- Rat (18)
- Escherichia coli (16)
- Blomia tropicalis (10)
- SARS-CoV-2 (8)
- Dermatophagoides pteronyssinus (6)
- Streptococcus Pyogenes (5)
- Staphylococcus aureus (4)
- Orf Virus (3)
- Bovine (2)
- Dermatophagoides farinae (2)
- Rabbit (2)
- Streptococcus sp. (2)
- Alternaria alternata (1)
- Betula pendula (1)
- E.coli (1)
- Enterobacteria phage T4 (1)
- Escherichia phage T7 (1)
- HIV (1)
- Hepatitis B Virus (1)
- Humulus japonicus (1)
- Lachnospiraceae bacterium ND2006 (1)
- Lepidoglyphus destructor (1)
- Leptotrichia wadei (1)
- Macaca fascicularis (1)
- Mucor hiemalis (1)
- Mus musculus (1)
- Saccharomyces cerevisiae (1)
- Serratia marcescens (1)
- Staphylococcus aureus subsp. (1)
- Streptomyces avidinii (1)
- Streptomyces plicatus (1)
- Tobacco etch virus (1)
- Vaccinia virus (1)
Target Symbol
- VEGFA (27)
- FGF2 (9)
- IL4 (9)
- IL6 (9)
- CSF2 (7)
- FABP4 (7)
- IL1B (7)
- LIF (7)
- TNF ALPHA (7)
- IL1 BETA (6)
- TGIF (6)
- TNF (6)
- TRIM72 (6)
- APOLIPOPROTEIN E (5)
- BDNF (5)
- BIRC5 (5)
- IL15 (5)
- INTERFERON GAMMA (5)
- PARATHYROID HORMONE (5)
- PDGFA (5)
- TXN (5)
- AIF (4)
- ALPHA SYNUCLEIN (4)
- BMP2 (4)
- BMP4 (4)
- EGR1 (4)
- EIF4E (4)
- EOTAXIN 3 (4)
- HIST1H2AK (4)
- IL33 (4)
- PADI4 (4)
- PCNA (4)
- PEROXIREDOXIN 1 (4)
- PITX2 (4)
- PTPN11 (4)
- RNASE9 (4)
- TPT1 (4)
- VILIP1 (4)
- VINCULIN (4)
- ZNF75A (4)
- ADA2A (3)
- ATP5F1C (3)
- BNP (3)
- C-MYC (3)
- C15ORF21 (3)
- CAMK2B (3)
- CANT1 (3)
- CASPASE 1 (3)
- CD8A (3)
- CDC42SE2 (3)
- CREM (3)
- DEOXYRIBONUCLEASE I LIKE 1 (3)
- DNTT (3)
- ELF2 (3)
- FAM104B (3)
- FERRITIN HEAVY CHAIN (3)
- FERRITIN LIGHT CHAIN (3)
- FGF8 (3)
- GATA3 (3)
- GFI1 (3)
- GREM1 (3)
- IL18 (3)
- IL3 (3)
- INHA (3)
- KITLG (3)
- LRPAP1 (3)
- LY6D (3)
- MET (3)
- MTHFD2 (3)
- MTX2 (3)
- MYH (3)
- N PROTEIN (3)
- NEUROFILAMENT (3)
- PDGFB (3)
- PDPN (3)
- PPP1A (3)
- PRAME (3)
- PREALBUMIN (3)
- SEPSECS (3)
- SINHCAF (3)
- SMIM29 (3)
- TCF4 (3)
- TCL6 (3)
- THPO (3)
- TRAIL (3)
- TRIB3 (3)
- WILMS TUMOR PROTEIN (3)
- XBP1 (3)
- XRCC3 (3)
- ZNF596 (3)
- ZNF610 (3)
- A4GNT (2)
- ACVRL1 (2)
- AFAP (2)
- AGO1 (2)
- AHCY (2)
- ALAS1 (2)
- ALKBH5 (2)
- ANGIOPOIETIN 1 (2)
- APOL3 (2)
Tags
- N-His (2088)
- N-GST and C-His (435)
- N-GST and C-HIS (150)
- Tag Free (130)
- N-His-ABP (Albumin-Binding Protein) (52)
- His-tag (49)
- N-His-Trx (18)
- C-His (13)
- N-GST, C-His (13)
- N-HIS (9)
- Tag-free (8)
- N-6His (6)
- N-GST (5)
- N-His PDCD1(Pro21-Val170) (5)
- N-His&C-His (4)
- N-His-GST (4)
- C-Avi&6His (3)
- C-HIS (3)
- His-tagged, myc-tagged (3)
- N-Avi (3)
- Myc-DDK (2)
- N-His-PDCD1(Pro21-Val170) (2)
- N-terminal His (2)
- N-terminal His-PDCD1(Pro21-Val170) tag (2)
- C-DDK (1)
- C-DDK-His (1)
- C-HIS/C (1)
- C-Myc/DDK (1)
- DDK-tag (1)
- His tag (1)
- N-AVI (1)
- N-DDK (1)
- N-FUSION (1)
- N-GFP (1)
- N-GST(CKM) and N-His(CKB) (1)
- N-HIS and C-AVIPLUS (1)
- N-PROTEIN (1)
- N-T7 (1)
- N-TRX (1)
- N-TRXA (1)
- N-Trx-His (1)
- N-terminal His-tag (1)
Families
- Druggable Genome (1315)
- Transcription Factors (527)
- Transmembrane (435)
- Secreted Protein (385)
- ES Cell Differentiation/IPS (155)
- Protein Kinase (135)
- Stem cell - Pluripotency (99)
- Protease (77)
- Adult stem cells (60)
- Embryonic stem cells (48)
- Phosphatase (35)
- Induced pluripotent stem cells (30)
- Cancer stem cells (27)
- Stem cell relevant signaling - TGFb/BMP signaling pathway (22)
- Ion Channels: Other (20)
- Stem cell relevant signaling - Wnt Signaling pathway (15)
- Stem cell relevant signaling - JAK/STAT signaling pathway (13)
- 10 mM Tris-HCl (12)
- GPCR (12)
- Nuclear Hormone Receptor (12)
- the solution can be further dialyzed against PBS (phosphate buffered saline (12)
- 2 mM dithiothreitol (10)
-
ELISA.
Western Blot. (10) - e.g. Dulbecco's PBS). (10)
- Ion Channels: Potassium (8)
- both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl (8)
- Stem cell relevant signaling - DSL/Notch pathway (7)
-
Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml (6) - P450 (6)
- pH 7.4). For immunization purposes (6)
- Ion Channels: Transient receptor potential (4)
-
Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml (4) -
pH 7.4).
For immunization purposes (4) -
pH 7.4).
For immunization purposes (4) - Ion Channels: Cys-loop Receptors (3)
-
Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoblotting.
Receptor-binding studies. (3) - Western Blot. (3)
- 0.1% (w/v) insulin (2)
- 10mM Tris-HCl (2)
- 1mM DTT (2)
- 2 mM dithiothreitol (2)
-
2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes (2) - 2mM dithiothreitol (2)
- 63mM sodium phosphate (2)
-
Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above) (2) - Can be used as standard in a Sandwich ELISA. (2)
- Ion Channels: Sodium (2)
-
Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above) (2) -
Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris (2) -
both dissolved in 9.5 M urea buffer (see above).
Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl (2) - e.g. Dulbeccos PBS). (2)
- e.g.Dulbecco's PBS). (2)
- protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl (2)
- protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl (2)
- the solution can be further dialyzed against PBS (Phosphate Buffered Saline (2)
-
ELISA.
Western blotting. (1) -
Positive control for Western blot analysis.
Standard for ELISA. (1) -
Adermann K. Role of the prosequence of guanylin. Protein Sci. 1999 Sep; 8(9): 1850-9.
4. Lauber T (1) -
Cohen MB. Proguanylin secretion and the role of negative-feedback inhibition in a villous epithelial cell line. Am J Physiol Gastrointest Liver Physiol. 2002 Sep; 283(3): G695-702.
3. Schulz A (1) - ELISA (1)
-
ELISA.
Western blotting. (1) - Forssmann WG (1)
- Hawkins JA (1)
- Hidaka Y (1)
- Ion Channels: ATP Receptors (1)
- Marx UC (1)
-
Marx UC. Native and recombinant proguanylin feature identical biophysical properties and are monomeric in solution. Biochemistry. 2002 Dec 10; 41(49): 14602-12.
2. Rudolph JA (1) - Marx UC. Solution structure of human proguanylin: the role of a hormone prosequence. J Biol Chem. 2003 Jun 27; 278(26): 24118-24. Epub 2003 Apr 21. (1)
- Neudecker P (1)
- Nourse A (1)
- Rosch P (1)
- Schulz A (1)
- Shimonishi Y (1)
-
Western Blot.
Protocol: 1. Lauber T (1) - Western Blotting. (1)
Pathways
- Pathways in cancer (170)
- Cytokine-cytokine receptor interaction (151)
- Metabolic pathways (138)
- MAPK signaling pathway (108)
- Hematopoietic cell lineage (82)
- Jak-STAT signaling pathway (77)
- Focal adhesion (75)
- Systemic lupus erythematosus (60)
- Regulation of actin cytoskeleton (58)
- T cell receptor signaling pathway (58)
- Apoptosis (55)
- Toll-like receptor signaling pathway (55)
- TGF-beta signaling pathway (53)
- Alzheimer's disease (52)
- Wnt signaling pathway (52)
- NOD-like receptor signaling pathway (50)
- Melanoma (49)
- Natural killer cell mediated cytotoxicity (47)
- Neurotrophin signaling pathway (44)
- Fc epsilon RI signaling pathway (42)
- Graft-versus-host disease (42)
- Cytosolic DNA-sensing pathway (41)
- Huntington's disease (41)
- Colorectal cancer (40)
- Hypertrophic cardiomyopathy (HCM) (39)
- Renal cell carcinoma (39)
- Pancreatic cancer (38)
- Prostate cancer (38)
- Melanogenesis (37)
- Cell cycle (33)
- Type I diabetes mellitus (33)
- Allograft rejection (32)
- ErbB signaling pathway (32)
- Adipocytokine signaling pathway (31)
- Amyotrophic lateral sclerosis (ALS) (31)
- Chemokine signaling pathway (31)
- Chronic myeloid leukemia (31)
- Endocytosis (31)
- Hedgehog signaling pathway (31)
- Prion diseases (31)
- Asthma (30)
- Bladder cancer (30)
- Dilated cardiomyopathy (30)
- Insulin signaling pathway (30)
- Ribosome (30)
- Small cell lung cancer (30)
- Parkinson's disease (29)
- Glioma (28)
- Oocyte meiosis (28)
- VEGF signaling pathway (28)
- Leukocyte transendothelial migration (27)
- mTOR signaling pathway (27)
- Acute myeloid leukemia (26)
- Basal cell carcinoma (26)
- Gap junction (25)
- p53 signaling pathway (25)
- GnRH signaling pathway (24)
- Calcium signaling pathway (23)
- Progesterone-mediated oocyte maturation (23)
- RIG-I-like receptor signaling pathway (23)
- B cell receptor signaling pathway (22)
- Cell adhesion molecules (CAMs) (22)
- Adherens junction (21)
- Autoimmune thyroid disease (21)
- Endometrial cancer (21)
- Oxidative phosphorylation (21)
- Complement and coagulation cascades (19)
- Neuroactive ligand-receptor interaction (19)
- Type II diabetes mellitus (19)
- ECM-receptor interaction (18)
- Epithelial cell signaling in Helicobacter pylori infection (18)
- Purine metabolism (18)
- Axon guidance (17)
- Pyrimidine metabolism (17)
- Ubiquitin mediated proteolysis (17)
- Vascular smooth muscle contraction (17)
- Antigen processing and presentation (16)
- Fc gamma R-mediated phagocytosis (16)
- Long-term potentiation (16)
- PPAR signaling pathway (16)
- Primary immunodeficiency (16)
- Base excision repair (15)
- Non-small cell lung cancer (15)
- Arrhythmogenic right ventricular cardiomyopathy (ARVC) (14)
- Long-term depression (13)
- Pathogenic Escherichia coli infection (13)
- Glycerophospholipid metabolism (11)
- Mismatch repair (11)
- Notch signaling pathway (11)
- Nucleotide excision repair (11)
- Olfactory transduction (11)
- Thyroid cancer (11)
- Tight junction (11)
- ABC transporters (10)
- Arginine and proline metabolism (10)
- Cardiac muscle contraction (10)
- RNA degradation (10)
- Regulation of autophagy (10)
- Spliceosome (10)
- Tyrosine metabolism (10)
- Viral myocarditis (10)
- Homologous recombination (9)
- Lysosome (9)
- Maturity onset diabetes of the young (9)
- Metabolism of xenobiotics by cytochrome P450 (9)
- Proteasome (9)
- Retinol metabolism (9)
- Tryptophan metabolism (9)
- Aminoacyl-tRNA biosynthesis (8)
- DNA replication (8)
- Dorso-ventral axis formation (8)
- Drug metabolism - cytochrome P450 (8)
- Glycolysis / Gluconeogenesis (8)
- SNARE interactions in vesicular transport (8)
- Arachidonic acid metabolism (7)
- Basal transcription factors (7)
- Drug metabolism - other enzymes (7)
- Glutathione metabolism (7)
- N-Glycan biosynthesis (7)
- Phenylalanine metabolism (7)
- Porphyrin and chlorophyll metabolism (7)
- Alanine (6)
- Glycine (6)
- Valine (6)
- Vibrio cholerae infection (6)
- aspartate and glutamate metabolism (6)
- serine and threonine metabolism (6)
- Cysteine and methionine metabolism (5)
- Ether lipid metabolism (5)
- Glycosphingolipid biosynthesis - lacto and neolacto series (5)
- Glyoxylate and dicarboxylate metabolism (5)
- Heparan sulfate biosynthesis (5)
- Lysine degradation (5)
- Nitrogen metabolism (5)
- Phosphatidylinositol signaling system (5)
- Sphingolipid metabolism (5)
- leucine and isoleucine degradation (5)
- Androgen and estrogen metabolism (4)
- Chondroitin sulfate biosynthesis (4)
- Citrate cycle (TCA cycle) (4)
- Fructose and mannose metabolism (4)
- Histidine metabolism (4)
- Keratan sulfate biosynthesis (4)
- Linoleic acid metabolism (4)
- Non-homologous end-joining (4)
- O-Glycan biosynthesis (4)
- One carbon pool by folate (4)
- Renin-angiotensin system (4)
- Selenoamino acid metabolism (4)
- Starch and sucrose metabolism (4)
- Taste transduction (4)
- Ubiquinone and other terpenoid-quinone biosynthesis (4)
- alpha-Linolenic acid metabolism (4)
- Butanoate metabolism (3)
- Circadian rhythm - mammal (3)
- Fatty acid metabolism (3)
- Folate biosynthesis (3)
- Inositol phosphate metabolism (3)
- Pantothenate and CoA biosynthesis (3)
- RNA polymerase (3)
- Riboflavin metabolism (3)
- Sulfur metabolism (3)
- Caffeine metabolism (2)
- D-Glutamine and D-glutamate metabolism (2)
- Glycosaminoglycan degradation (2)
- Glycosylphosphatidylinositol(GPI)-anchor biosynthesis (2)
- Limonene and pinene degradation (2)
- Other glycan degradation (2)
- Phenylalanine (2)
- Pyruvate metabolism (2)
- Steroid biosynthesis (2)
- Terpenoid backbone biosynthesis (2)
- beta-Alanine metabolism (2)
- leucine and isoleucine biosynthesis (2)
- tyrosine and tryptophan biosynthesis (2)
- Amino sugar and nucleotide sugar metabolism (1)
- Ascorbate and aldarate metabolism (1)
- Biosynthesis of unsaturated fatty acids (1)
- C21-Steroid hormone metabolism (1)
- Cyanoamino acid metabolism (1)
- Fatty acid elongation in mitochondria (1)
- Galactose metabolism (1)
- Glycerolipid metabolism (1)
- Glycosphingolipid biosynthesis - ganglio series (1)
- Lipoic acid metabolism (1)
- Methane metabolism (1)
- Nicotinate and nicotinamide metabolism (1)
- Pentose and glucuronate interconversions (1)
- Pentose phosphate pathway (1)
- Primary bile acid biosynthesis (1)
- Propanoate metabolism (1)
- Protein export (1)
- Synthesis and degradation of ketone bodies (1)
