Chicken IgG (H&L) Antibody Texas Red™ Conjugated Pre-Adsorbed
| Applications | IF: 1:1,000 - 1:5,000 FLISA: 1:10,000 - 1:50,000 |
| Conjugation | Texas Red |
Products
Chicken IgG / Chicken IgY (H+L chain) rabbit polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: Western blot: 1/1,000-1/10,000. ELISA: 1/10,000-1/50,000. Immunohistochemistry: 1/500-1/2500. |
| Reactivities | Chicken |
| Conjugation | HRP |
Guinea pig IgG (H+L chain) rabbit polyclonal antibody, TRITC
| Applications | Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. Recommended Dilutions: FLISA: 1/10,000-1/50,000. IF Microscopy: 1/1,000-1/5,000. Flow Cytometry: 1/500-1/2,500. |
| Reactivities | Guinea Pig |
| Conjugation | TRITC |
Guinea pig IgG (H+L chain) rabbit polyclonal antibody, FITC
| Applications | This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. Recommended Dilutions: FLISA: 1/10,000-1/50,000. Flow Cytometry: 1/500-1/2,500. IF Microscopy: 1/1,000-1/5,000. |
| Reactivities | Guinea Pig |
| Conjugation | FITC |
Mouse IgG (H+L chain), F(ab)2 Fragment goat polyclonal antibody, Biotin
| Applications | Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Note: This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/10,000 to 1/150,000 of the reconstitution concentration is suggested for this product. Recommended Dilutions ELISA: 1/20,000-1/100,000. Immunohistochemistry: 1/1,000-1/5,000. Western blot: 1/2,000-1/10,000. |
| Reactivities | Mouse |
| Conjugation | Biotin |
Human IgG (F(ab)2 specific) rabbit polyclonal antibody, HRP
| Applications | Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/10,000-1/50,000. Western Blot: 1/1,000-1/10,000. Immunohistochemistry: 1/500-2,500. This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/20,000 to 1/100,000 of the reconstitution concentration is suggested for this product. |
| Reactivities | Human |
| Conjugation | HRP |
Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays. Recommended Dilutions: ELISA: 1/10,000-1/150,000. Western blot: 1/1,000-1/5,000. Immunohistochemistry: 1/1,000-1/10,000. |
| Reactivities | Chicken |
| Conjugation | HRP |
Hamster IgG goat polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. This product has been assayed against 1.0 µg of Hamster IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:15,000 to 1:65,000 of the reconstitution concentration is suggested for this product. |
| Reactivities | Hamster |
| Conjugation | HRP |
Human IgG (gamma chain specific) rabbit polyclonal antibody, Biotin
| Applications | Suitable for Immunoblotting, ELISA, Immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. Recommended Dilutions: This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:5,000 to 1:35,000 of the reconstitution concentration is suggested for this product. |
| Reactivities | Human |
| Conjugation | Biotin |
Rabbit IgG (H+L chain) sheep polyclonal antibody, PE
| Applications | Flow Cytometry (Use 50 µl of 1/5-1/10 diluted antibody to label 10e6 cells in 100 µl). |
| Reactivities | Rabbit |
| Conjugation | PE |
Human IgE (Fc specific) goat polyclonal antibody, Azide Free
| Applications | This antiserum is intended to detect and identify IgE in serum or other body fluids using the immunofixation technique. Like immunoelectrophoresis immunofixation is essentially a two step technique. Proteins in a complex mixture are separated by electrophoresis in a gel carrier, followed by immunoprecipitation in situ with the antiserum. Non-precipitated proteins are removed by washing and the precipitated complex is revealed with a protein stain which allows its exact localization. Immunofixation may be the method of choice whenever a high level of sensitivity is required to identify a minor protein component against a high background of other proteins. It enables the detection and identification of more than one paraproteins in serum or of free light chain. The detection limit is approximately 0.5 to 1 mg/ml in the presence of normal levels of immunoglobulins. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used. Antibody titre: Precipitin titre not less than 1/16 when tested against an appropriate reference in agar immunodiffusion block titration. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Mouse IgG (H+L chain) porcine polyclonal antibody, Serum
| Reactivities | Mouse |
| Conjugation | Unconjugated |
Canine IgG (Fc specific) goat polyclonal antibody, Azide Free
| Applications | Can be used as unlabelled primary or secondary reagent for indirect detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to prepare conjugates of the user’s own choice; to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used. Typical working dilutions: In histochemistry are usually between 1/50 and 1/250. In ELISA and comparable non-precipitating antibody-binding assays between 1/1000 and 1/5000. |
| Reactivities | Canine |
| Conjugation | Unconjugated |
Canine IgG (Fc specific) goat polyclonal antibody, Biotin
| Applications | Can be used in immunocytochemical and immunohistochemical use for the detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using an reference antibody of goat origin known to be of the IgG isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG in dog serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions: For histochemical and cytochemical use are usually between 1/100 and 1/250. In ELISA and comparable non-precipitating antibody-binding assays between 1/500 and 1/5000. |
| Reactivities | Canine |
| Conjugation | Biotin |
Canine IgA (Fc specific) goat polyclonal antibody, Biotin
| Applications | Can be used in immunocytochemical and immunohistochemical staining of IgA at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgA antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of dog origin known to be of the IgA isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgA in dog serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions: For histochemical and cytochemical use are usually between 1/100 and 1/500. In ELISA and comparable non-precipitating antibody-binding assays between 1/1000 and 1/5000. |
| Reactivities | Canine |
| Conjugation | Biotin |
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Reactivities
- Mouse (396)
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- Rat (128)
- Goat (79)
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- Sheep (64)
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- Golden Syrian Hamster (7)
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- Chimpanzee (4)
- Mouse IgG (3)
- Human IgG (2)
- Reptiles (2)
- Goat IgG (1)
- Mammalian (1)
- Rabbit IgG (1)
