Cas9 expression vector in AAVS1 donor vector, blasticidin selection (10ug)
| Function | Cas9 only vectors |
Cas9 expression vector in AAVS1 donor vector, blasticidin selection (10ug)
| Function | Cas9 only vectors |
pCMV6-Entry2, mammalian vector with C-terminal Myc-DDK tag, 10ug
| C-Tag | Myc-DDK |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Cell Selection | Neomycin |
AAVS1 donor vector with AAVS1 homologous arms, tRFP under EF1a promoter, and Blasticidin selection (10 ug)
| Function | AAVS1 donor vector |
HuSH shRNA Retroviral Cloning Vector with blasticidin resistance (pB-RS Vector), 5 ug
| Viral Packaging | Retroviral |
pCas-Guide-Puro-CRISPRa-Scramble, scramble gRNA control in GE100081 vector (with Puro selection)
pCMV6-AN-FP635, mammalian vector with N-terminal FP635, 10ug
| N-Tag | TurboFP635 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Cell Selection | Neomycin |
pLenti-E/hSyn-C-mGFP-P2A-Puro, Lenti vector with CMV enhancer, Human synapsin I promoter, C-terminal mGFP tag and P2A-Puro, 10ug
| C-Tag | mGFP |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Cell Selection | Puromycin |
pLenti-hSyn-C-mGFP-P2A-Puro, Lenti vector with Human synapsin I promoter, C-terminal mGFP tag and P2A-Puro, 10ug
| C-Tag | mGFP |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Cell Selection | Puromycin |
pLenti-E/hSyn-C-Myc-DDK-P2A-Puro, Lenti vector with CMV enhancer, Human synapsin I promoter, C-terminal Myc-DDK tag and P2A-Puro, 10 ug
| C-Tag | Myc-DDK |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Cell Selection | Puromycin |
p-xCas9-Guide vector (with xCas9 expression) for genomic target sequence cloning (10 µg)
| Function | All-in-One CRISPR/Cas9 vectors |
pCas-Guide-CRISPRa-Scramble, scramble gRNA control in GE100055 vector (no selection marker/reporter)
| Function | CRISPRa scramble control vector |
pLenti-Cas9-mGFP-P2A-Puro control plasmid containing the expression cassette for Cas9-mGFP fusion protein and Puromycin resistance.
| Function | Cas9 only vectors |
CRISPRa SAM vector kit containing pCas-Guide-CRISPRa (GE100055), pCRISPRa-Enhancer (GE100056) and pCas-Guide-CRISPRa-Scramble (GE100058)
| Function | CRISPRa SAM vector kit |
29-mer Scrambled shRNA Cassette in p-RFP-C-shLenti Vector, 5 ug
| Viral Packaging | Lentiviral |
HuSH shRNA RFP Lenti Cloning Vector (pRFP-C-shLenti Vector), 5 ug
| Viral Packaging | Lentiviral |