Vector kit to insert Cas9 at AAVS1 locus (puro selection), including AAVS1 gRNA vector (cat# GE100023), Cas9-Puro donor (cat# GE100037), and a scramble gRNA control (cat# GE100003)
Function | CRISPR/AAVS1 kit |
Vector kit to insert Cas9 at AAVS1 locus (puro selection), including AAVS1 gRNA vector (cat# GE100023), Cas9-Puro donor (cat# GE100037), and a scramble gRNA control (cat# GE100003)
Function | CRISPR/AAVS1 kit |
pCas-Guide-Puro-CRISPRi-Scramble, scramble gRNA control in GE100083 vector (with Puro selection)
pAAV-AC-GFP, AAV vector with C-terminal tGFP tag and EF1a promoter
HuSH shRNA Retroviral Cloning Vector with blasticidin resistance (pB-RS Vector), 5 ug
Viral Packaging | Retroviral |
pLenti-E/hSyn-C-Myc-DDK-P2A-Puro, Lenti vector with CMV enhancer, Human synapsin I promoter, C-terminal Myc-DDK tag and P2A-Puro, 10 ug
C-Tag | Myc-DDK |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Cell Selection | Puromycin |
HuSH shRNA RFP Lenti Cloning Vector (pRFP-C-shLenti Vector), 5 ug
Viral Packaging | Lentiviral |
29-mer Scrambled shRNA Cassette in p-RFP-C-shLenti Vector, 5 ug
Viral Packaging | Lentiviral |
pLenti-Cas-Guide vector (with Cas9 expression) for genomic target sequence cloning (10 µg), GE100008 included.
Function | All-in-One CRISPR/Cas9 vectors |
29-mer Scrambled shRNA Cassette in pGFP-A-shAAV Vector, 5ug
Viral Packaging | Adeno-associated virus (AAV) |
pLenti-Cas9-Myc-DDK-P2A-Puro control plasmid containing the expression cassette for Cas9-Myc-DDK fusion protein and Puromycin resistance.
Function | Cas9 only vectors |
Validated sgRNA targeting RFP dead mutant in GE100041C cells
CRISPRa SAM vector kit containing pCas-Guide-CRISPRa (GE100055), pCRISPRa-Enhancer (GE100056) and pCas-Guide-CRISPRa-Scramble (GE100058)
Function | CRISPRa SAM vector kit |