pAAV-AC-GFP, AAV vector with C-terminal tGFP tag
Vectors
AAVS1 donor vector with AAVS1 homologous arms cloned (Puro selection), EF1a promoter, ready for exogenous gene cloning (10 ug)
Function | AAVS1 donor vector |
pT7-Guide-IVT vector for target sequence cloning; IVT to produce gRNA, (10ug)
Function | CRISPR/Cas9 T7 Vectors |
pCMV6-AC-IRES-GFP, mammalian vector with IRES-driven tGFP expression, 10ug
C-Tag | Myc-DDK |
E. coli Selection | Ampicillin (100 ug/mL) |
Cell Selection | Neomycin |
pCMV6-AC-HA-His, mammalian vector with C-terminal HA-His tag, 10ug
C-Tag | HA-His |
E. coli Selection | Ampicillin (100 ug/mL) |
Cell Selection | Neomycin |
pCMV6-AN-GFP-C-His, mammalian vector with N-GFP and C-His double tags, 10ug
C-Tag | His |
N-Tag | tGFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Cell Selection | Neomycin |
pCMV6-AC-DDK-His, mammalian vector with C-terminal DDK-His tag, 10ug
C-Tag | DDK-His |
E. coli Selection | Ampicillin (100 ug/mL) |
Cell Selection | Neomycin |
pCMV6-AC-Myc-DDK, mammalian vector with C-terminal Myc-DDK tag, 10ug
C-Tag | Myc-DDK |
E. coli Selection | Ampicillin (100 ug/mL) |
Cell Selection | Neomycin |
pEX-C-His, bacterial expression vector with C-terminal His tag, 10ug
C-Tag | His |
E. coli Selection | Ampicillin (100 ug/mL) |
HuSH shRNA RFP Lenti Cloning Vector with blasticidin resistance (pRFP-CB-shLenti Vector), 5 ug
Viral Packaging | Lentiviral |
HuSH shRNA GFP AAV Cloning Vector (pGFP-A-shAAV Vector), 5 ug
Viral Packaging | Adeno-associated virus (AAV) |
pEX-N-His-GST, bacterial expression vector with N-terminal His-GST tag, 10ug
N-Tag | His-GST |
E. coli Selection | Ampicillin (100 ug/mL) |
pLenti-C-tRFP, Lenti Vector with C-terminal tRFP tag, 10ug
C-Tag | tRFP |
E. coli Selection | Chloramphenicol (34 ug/mL) |
pLenti-EF1a-C-tGFP, Lenti Vector with EF1 alpha promoter and C-terminal tGFP tag, 10ug
C-Tag | tGFP |
E. coli Selection | Chloramphenicol (34 ug/mL) |
pCMV6-AN-mRFP, mammalian vector with N-terminal mRFP, 10ug
N-Tag | mRFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Cell Selection | Neomycin |