CRISPR Vectors
pCas-Guide-Nickase vector for gRNA cloning (with Cas9 D10A expression) (10 µg)
| Function | Cas9 Nickase |
Cas9 expression vector in AAVS1 donor vector, puromycin selection (10 ug)
| Function | Cas9 only vectors |
pCRISPRa-Enhancer vector, expressing MS2-p65-HSF1, which synergistically activates gene expression with dCas9-VP64.
| Function | CRISPRa Enhancer Vector |
pLenti-Cas9-P2A-tGFP, Cas9 in lenti vector with tGFP after P2A (10 ug)
| Function | Cas9 only vectors |
pLenti-Cas9 vector, Cas9 in lenti vector (10 ug)
| Function | Cas9 only vectors |
pT7-Cas9-Nickase vector (Cas9 D10A) (10 µg)
| Function | Cas9 Nickase |
Vector kit to insert Cas9 at AAVS1 locus (puro selection), including AAVS1 gRNA vector (cat# GE100023), Cas9-Puro donor (cat# GE100037), and a scramble gRNA control (cat# GE100003)
| Function | CRISPR/AAVS1 kit |
pCas-Guide-Puro-CRISPRi-Scramble, scramble gRNA control in GE100083 vector (with Puro selection)
pLenti-Cas-Guide vector (with Cas9 expression) for genomic target sequence cloning (10 µg), GE100008 included.
| Function | All-in-One CRISPR/Cas9 vectors |
GE100010 is the updated version of GE100009.GE100010 is an improved version of GE100009.
pLenti-Cas9-Myc-DDK-P2A-Puro control plasmid containing the expression cassette for Cas9-Myc-DDK fusion protein and Puromycin resistance.
| Function | Cas9 only vectors |
Validated sgRNA targeting RFP dead mutant in GE100041C cells
CRISPRa SAM vector kit containing pCas-Guide-CRISPRa (GE100055), pCRISPRa-Enhancer (GE100056) and pCas-Guide-CRISPRa-Scramble (GE100058)
| Function | CRISPRa SAM vector kit |
