Leukemia inhibitory factor human protein, 5 µg
| Expression Host | E. coli |
Recombinant Proteins
Leukemia inhibitory factor human protein, 10 µg
| Expression Host | E. coli |
NGF human protein, 5 µg
| Expression Host | CHO |
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Proteins
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Species
- Human (32136)
- Mouse (7078)
- SARS-CoV-2 (37)
- Rat (29)
- Cynomolgus (21)
- Escherichia coli (18)
- Blomia tropicalis (10)
- Bovine (7)
- Dermatophagoides pteronyssinus (6)
- Orf Virus (5)
- Staphylococcus aureus (5)
- Streptococcus Pyogenes (5)
- Hepatitis B Virus (3)
- Chicken (2)
- Dermatophagoides farinae (2)
- Rabbit (2)
- Rhesus (2)
- Streptococcus sp. (2)
- Alternaria alternata (1)
- Betula pendula (1)
- Chlorocebus aethiops (1)
- Drosophila melanogaster (1)
- E.coli (1)
- Enterobacteria phage T4 (1)
- Escherichia phage T7 (1)
- HIV (1)
- Human herpesvirus (1)
- Human papillomavirus (1)
- Humon (1)
- Humulus japonicus (1)
- Lachnospiraceae bacterium ND2006 (1)
- Lepidoglyphus destructor (1)
- Leptotrichia wadei (1)
- Macaca fascicularis (1)
- Mucor hiemalis (1)
- Mus musculus (1)
- Proteobacteria (1)
- SIV (1)
- Saccharomyces cerevisiae (1)
- Serratia marcescens (1)
- Staphylococcus aureus subsp. (1)
- Streptococcus (1)
- Streptomyces avidinii (1)
- Streptomyces plicatus (1)
- Tobacco etch virus (1)
- Vaccinia virus (1)
- viral (1)
Target Symbol
- VEGFA (40)
- ALPHA 1 ANTITRYPSIN (32)
- NCALD (25)
- NDRG2 (23)
- S PROTEIN (23)
- IRF5 (22)
- RIBONUCLEASE INHIBITOR (21)
- FGFR1 (20)
- HMGA1 (19)
- ISOCITRATE DEHYDROGENASE (19)
- PQBP1 (19)
- PRKAR1B (19)
- TPM1 (19)
- MTH1 (18)
- MYOSIN (18)
- ALKALINE PHOSPHATASE (17)
- ALPHA SYNUCLEIN (16)
- B3GALT5 (16)
- CPNE1 (16)
- FHL1 (16)
- MBNL1 (16)
- MORTALITY FACTOR 4 LIKE 2 (16)
- PCBP2 (16)
- UBE2D3 (16)
- 14-3-3 ZETA (15)
- ASPARTATE BETA HYDROXYLASE (15)
- FGF1 (15)
- HNF 4 ALPHA (15)
- HNRNP F (15)
- IL6 (15)
- M-CSF (15)
- MYELIN BASIC PROTEIN (15)
- NDUF3 (15)
- P38 (15)
- S100 CALCIUM BINDING PROTEIN A13 (15)
- ANNEXIN A2 (14)
- AURORA A (14)
- CD36 (14)
- FGF2 (14)
- FHL2 (14)
- HER2 (14)
- IDH2 (14)
- MAX (14)
- PGF (14)
- APOLIPOPROTEIN E (13)
- B RAF (13)
- CARDIAC TROPONIN T (13)
- CD10 (13)
- COASY (13)
- DGKA (13)
- ESTROGEN RELATED RECEPTOR GAMMA (13)
- FGL1 (13)
- HNRNPD (13)
- HOMER2 (13)
- ICAM2 (13)
- IL4 (13)
- LMO2 (13)
- MBNL3 (13)
- MPST (13)
- PEROXIREDOXIN 1 (13)
- RAB27A (13)
- RNF14 (13)
- TMEM169 (13)
- UBE2I (13)
- YPEL5 (13)
- ZFYVE27 (13)
- AKT1 (12)
- ALDOLASE (12)
- C2ORF88 (12)
- C4BPB (12)
- CATHEPSIN B (12)
- CD33 (12)
- CSF2 (12)
- DOUBLECORTIN (12)
- EGFR (12)
- FCGRT (12)
- GIPC (12)
- GM CSF RECEPTOR ALPHA (12)
- GMPR2 (12)
- IKK GAMMA (12)
- JNK1 (12)
- LIF (12)
- OSBPL9 (12)
- P53 (12)
- PAX2 (12)
- PPAR GAMMA (12)
- QK1 (12)
- SEPTIN6 (12)
- SIRP ALPHA (12)
- SPART (12)
- SULT1A1 (12)
- SUPT5H (12)
- TNF ALPHA (12)
- TROPOMYOSIN 3 (12)
- UBE2C (12)
- WDR79 (12)
- ACRV1 (11)
- BAIAP2 (11)
- BETA CATENIN (11)
- BREAST CANCER SUPPRESSOR CANDIDATE 1 (11)
Expression Hosts
Tags
- C-Myc/DDK (27880)
- C-MYC/DDK (6899)
- N-His (2118)
- N-GST and C-His (441)
- C-Human Fc (268)
- C-His (205)
- C-DDK (201)
- C-6×His (174)
- N-GST and C-HIS (150)
- Tag Free (144)
- C-6His (119)
- C-DDK/His (104)
- His-tag (75)
- Myc-DDK (67)
- C-Mouse Fc (54)
- N-His-ABP (Albumin-Binding Protein) (52)
- N-Human Fc (51)
- C-HIS (49)
- His-Tag (42)
- C-Mouse Fc and 6×His (32)
- C-Fc (30)
- C-8His (28)
- C-hFc (22)
- N-His-Trx (18)
- N-6His (17)
- C-Human Fc and 6×His (16)
- N-GST, C-His (13)
- hIgG-His-Tag (12)
- N-HIS (10)
- C- 6×His (8)
- N-6×His (8)
- Tag-free (8)
- C-Avi&6His (7)
- C-10His (6)
- N-GST (6)
- hIgG-Tag (6)
- N-His PDCD1(Pro21-Val170) (5)
- N-His/DDK (5)
- N-Mouse Fc and C-6×His (5)
- C-6×His tag and C-Human Fc (4)
- C-mouse Fc (4)
- N-8His (4)
- N-Avi (4)
- N-His&C-His (4)
- N-His-GST (4)
- C-Fc/His (3)
- C-hFc&His (3)
- His-tagged, myc-tagged (3)
- N-DDK (3)
- C-3×Flag Tag (2)
- C-6xHis (2)
- C-6xHis/AVI (2)
- C-7×His (2)
- C-DDK-His (2)
- C-HUMAN (2)
- N-8His&N-FLAG (2)
- N-Fc-FLAG (2)
- N-His-PDCD1(Pro21-Val170) (2)
- N-Mouse Fc (2)
- N-terminal His (2)
- N-terminal His-PDCD1(Pro21-Val170) tag (2)
- C-AVI-PLUS (1)
- C-Avi-His (1)
- C-DDK/10xHis (1)
- C-DDK/6xHis (1)
- C-FC (1)
- C-FC/6xHis (1)
- C-FLAG-Fc (1)
- C-Fc Human (1)
- C-Fc Mouse (1)
- C-HIS/C (1)
- C-HIS/DDK (1)
- C-His/AVIplus (1)
- C-His/DDK (1)
- C-Human Fc, Avi (1)
- C-Human Fc-Avi (1)
- C-hIgG1 Fc (1)
- C-hIgG4P Fc (1)
- C-mFc (1)
- C-terminal DDK (1)
- C-terminal His (1)
- DDK-tag (1)
- His tag (1)
- N-10*His (1)
- N-6His-FLAG (1)
- N-AVI (1)
- N-Avi&6His (1)
- N-DDK/His (1)
- N-FLAG (1)
- N-FUSION (1)
- N-Flag&C-His (1)
- N-GFP (1)
- N-GST(CKM) and N-His(CKB) (1)
- N-GST/C-His (1)
- N-HIS and C-AVIPLUS (1)
- N-PROTEIN (1)
- N-T7 (1)
- N-TRX (1)
- N-TRXA (1)
- N-Trx-His (1)
- N-hFc (1)
- N-mFc (1)
- N-terminal His-tag (1)
- N-terminal murine Fc Tag (1)
- No (1)
- Tag free (1)
Mutants
Brands
QC Levels
Families
- Druggable Genome (13758)
- Transmembrane (5730)
- Transcription Factors (3137)
- Secreted Protein (2756)
- Protein Kinase (1399)
- ES Cell Differentiation/IPS (996)
- Protease (849)
- Stem cell - Pluripotency (839)
- Phosphatase (397)
- Adult stem cells (225)
- Ion Channels: Other (216)
- Embryonic stem cells (164)
- Nuclear Hormone Receptor (152)
- P450 (131)
- GPCR (114)
- Cancer stem cells (96)
- Induced pluripotent stem cells (90)
- Stem cell relevant signaling - JAK/STAT signaling pathway (88)
- ELISA (55)
- Stem cell relevant signaling - TGFb/BMP signaling pathway (52)
- Stem cell relevant signaling - Wnt Signaling pathway (45)
- Stem cell relevant signaling - DSL/Notch pathway (44)
- SDS-PAGE (42)
- Ion Channels: Potassium (35)
- Bioactivity (27)
- Ion Channels: Glutamate Receptors (19)
- the solution can be further dialyzed against PBS (phosphate buffered saline (14)
- Ion Channels: ATP Receptors (13)
- 10 mM Tris-HCl (12)
- 2 mM dithiothreitol (12)
- Enzyme Activity (12)
- Ion Channels: Cys-loop Receptors (12)
- e.g. Dulbecco's PBS). (12)
- Flow Cyt (11)
-
ELISA.
Western Blot. (10) - Ion Channels: Transient receptor potential (10)
- Ion Channels: Sodium (8)
- both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl (8)
- pH 7.4). For immunization purposes (8)
-
Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml (6) -
Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml (4) -
pH 7.4).
For immunization purposes (4) -
pH 7.4).
For immunization purposes (4) - Ion Channels: Cyclic nucleotide gated (3)
-
Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoblotting.
Receptor-binding studies. (3) - Western Blot. (3)
- 0.1% (w/v) insulin (2)
- 000-1/5 (2)
- 10 mM sodium phosphate (2)
- 10mM Tris-HCl (2)
- 1mM DTT (2)
- 2 mM dithiothreitol (2)
-
2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes (2) - 2mM dithiothreitol (2)
- 63mM sodium phosphate (2)
- Biological activity/ Working concentration: The working concentration of ECGF for HUVEC is in the range of 25µg/ml to 100µg/ml. When adding Heparin (2.5mg per mg ECGF) an ECGF concentration of 12µg/ml (30µg/ml Heparin) is optimal. In this case 6mg ECGF are sufficient for 500 ml medium. (2)
-
Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above) (2) - Can be used as standard in a Sandwich ELISA. (2)
- ELISA. (2)
- FACS (2)
-
Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above) (2) -
Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after vimentin is dissolved in 6 M urea buffer (see above) (2) - Protocol: 1. Uehara Y. (2)
-
Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris (2) -
and Fukazawa H. (1991) Methods Enzymol. 201: 370.
2. Fukazawa H. (2) - and Nuccitelli R. (1992) J.Biol.Chem. 267:5608. (2)
-
both dissolved in 9.5 M urea buffer (see above).
Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl (2) - e.g. Dulbeccos PBS). (2)
- e.g.Dulbecco's PBS). (2)
-
et al. (1991) Biochem. Pharmacol. 42: 1661.
3. Satoh T. (2) -
et al.(1992) J.Biol.Chem. 267: 2537.
4. Weiss R. (2) - protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl (2)
- protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl (2)
- protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl (2)
- the solution can be further dialyzed against PBS (Phosphate Buffered Saline (2)
- 000) as well as other antibody based fluorescent assays. (1)
-
000)
Flow Cytometry/FACS (1/1 (1) - 000-1/200 (1)
- 000-1/40 (1)
-
000.
Immunohistochemistry: 1/1 (1) -
000.
Protein G Peroxidase is a useful reagent in Western Blotting and ELISA experiments. Protein G Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary antibody and appropriate substrate (such as TMB-1000 or Femtomax-110). (1) -
000.
Western blot: 1/10 (1) - Biological activity/ Working concentration: Optimum concentration for human umbilical vein endothelial cells (HUVEC) range from 50-200 μg/ml (1)
-
ELISA.
Western Blot.
Cell culture and/or animal studies. (1) -
ELISA.
Western blotting. (1) - Functional Assays. (1)
- Positive control for Western blot analysis (1)
-
Positive control for Western blot analysis.
Standard for ELISA. (1) -
Adermann K. Role of the prosequence of guanylin. Protein Sci. 1999 Sep; 8(9): 1850-9.
4. Lauber T (1) -
Cell culture and/or animal studies.
ELISA.
Western Blot. (1) -
Cohen MB. Proguanylin secretion and the role of negative-feedback inhibition in a villous epithelial cell line. Am J Physiol Gastrointest Liver Physiol. 2002 Sep; 283(3): G695-702.
3. Schulz A (1) -
ELISA.
Functional Assays. (1) -
ELISA.
Western blotting. (1) - Forssmann WG (1)
- Hawkins JA (1)
- Hidaka Y (1)
- Immunofluorescence Microscopy (1/1 (1)
-
Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-biotin-avidin based enzymatic assays.
Recommended Dilutions:
ELISA: 1/20 (1) - Marx UC (1)
-
Marx UC. Native and recombinant proguanylin feature identical biophysical properties and are monomeric in solution. Biochemistry. 2002 Dec 10; 41(49): 14602-12.
2. Rudolph JA (1) - Marx UC. Solution structure of human proguanylin: the role of a hormone prosequence. J Biol Chem. 2003 Jun 27; 278(26): 24118-24. Epub 2003 Apr 21. (1)
- Neudecker P (1)
- Nourse A (1)
- Rosch P (1)
- Schulz A (1)
- Shimonishi Y (1)
- Suitable for Immunoblotting (Western or dot blot) (1)
-
Western Blot.
ELISA. (1) -
Western Blot.
Protocol: 1. Lauber T (1) - Western Blotting. (1)
- optimal concentration with heparin (30 μg/ml) is about 12 μg/ml. (1)
Pathways
- Metabolic pathways (2610)
- Pathways in cancer (1080)
- MAPK signaling pathway (863)
- Cytokine-cytokine receptor interaction (769)
- Focal adhesion (656)
- Regulation of actin cytoskeleton (625)
- Endocytosis (544)
- Chemokine signaling pathway (492)
- Neurotrophin signaling pathway (469)
- Jak-STAT signaling pathway (431)
- Insulin signaling pathway (412)
- Purine metabolism (404)
- Natural killer cell mediated cytotoxicity (395)
- T cell receptor signaling pathway (393)
- Toll-like receptor signaling pathway (393)
- Alzheimer's disease (374)
- Ubiquitin mediated proteolysis (371)
- Hematopoietic cell lineage (365)
- Wnt signaling pathway (352)
- Cell adhesion molecules (CAMs) (345)
- Leukocyte transendothelial migration (340)
- Cell cycle (336)
- Axon guidance (334)
- ErbB signaling pathway (333)
- Pancreatic cancer (329)
- Huntington's disease (328)
- Tight junction (321)
- Progesterone-mediated oocyte maturation (316)
- Prostate cancer (315)
- Apoptosis (295)
- GnRH signaling pathway (295)
- Fc epsilon RI signaling pathway (289)
- Colorectal cancer (287)
- Calcium signaling pathway (284)
- Adherens junction (282)
- Oocyte meiosis (281)
- Fc gamma R-mediated phagocytosis (279)
- Melanoma (275)
- Renal cell carcinoma (272)
- Chronic myeloid leukemia (269)
- Hypertrophic cardiomyopathy (HCM) (269)
- Spliceosome (267)
- Dilated cardiomyopathy (264)
- VEGF signaling pathway (264)
- Pyrimidine metabolism (262)
- B cell receptor signaling pathway (260)
- Lysosome (254)
- Small cell lung cancer (249)
- TGF-beta signaling pathway (246)
- NOD-like receptor signaling pathway (243)
- Glioma (241)
- RIG-I-like receptor signaling pathway (238)
- Adipocytokine signaling pathway (235)
- Systemic lupus erythematosus (234)
- Epithelial cell signaling in Helicobacter pylori infection (232)
- Parkinson's disease (224)
- Gap junction (215)
- Vascular smooth muscle contraction (215)
- PPAR signaling pathway (214)
- Bladder cancer (212)
- Melanogenesis (212)
- Acute myeloid leukemia (211)
- Glycolysis / Gluconeogenesis (210)
- Endometrial cancer (208)
- Pathogenic Escherichia coli infection (206)
- Non-small cell lung cancer (205)
- Complement and coagulation cascades (203)
- Phosphatidylinositol signaling system (202)
- Oxidative phosphorylation (198)
- Long-term potentiation (193)
- Cytosolic DNA-sensing pathway (192)
- Antigen processing and presentation (191)
- p53 signaling pathway (191)
- Long-term depression (186)
- Arrhythmogenic right ventricular cardiomyopathy (ARVC) (174)
- mTOR signaling pathway (173)
- Amyotrophic lateral sclerosis (ALS) (171)
- Drug metabolism - cytochrome P450 (170)
- Arginine and proline metabolism (169)
- Metabolism of xenobiotics by cytochrome P450 (167)
- ECM-receptor interaction (166)
- Viral myocarditis (162)
- Cardiac muscle contraction (156)
- Inositol phosphate metabolism (155)
- Neuroactive ligand-receptor interaction (148)
- Thyroid cancer (146)
- Type II diabetes mellitus (146)
- Glycerophospholipid metabolism (144)
- Type I diabetes mellitus (144)
- Glutathione metabolism (143)
- Amino sugar and nucleotide sugar metabolism (142)
- Autoimmune thyroid disease (138)
- Retinol metabolism (136)
- Vibrio cholerae infection (135)
- Valine (134)
- Tyrosine metabolism (132)
- Starch and sucrose metabolism (131)
- Drug metabolism - other enzymes (128)
- Proteasome (121)
- leucine and isoleucine degradation (121)
- Alanine (119)
- Allograft rejection (119)
- Graft-versus-host disease (119)
- aspartate and glutamate metabolism (119)
- Tryptophan metabolism (118)
- Pyruvate metabolism (116)
- Fructose and mannose metabolism (115)
- RNA degradation (115)
- Prion diseases (114)
- Ribosome (114)
- Cysteine and methionine metabolism (113)
- Arachidonic acid metabolism (112)
- Fatty acid metabolism (111)
- Androgen and estrogen metabolism (110)
- Glycerolipid metabolism (110)
- Nucleotide excision repair (104)
- Hedgehog signaling pathway (101)
- Porphyrin and chlorophyll metabolism (100)
- Citrate cycle (TCA cycle) (96)
- Butanoate metabolism (90)
- Pentose phosphate pathway (89)
- Lysine degradation (87)
- DNA replication (86)
- Histidine metabolism (86)
- Base excision repair (85)
- Regulation of autophagy (85)
- Galactose metabolism (84)
- Notch signaling pathway (84)
- Olfactory transduction (84)
- Primary immunodeficiency (84)
- Propanoate metabolism (83)
- Nitrogen metabolism (81)
- Sphingolipid metabolism (81)
- Aminoacyl-tRNA biosynthesis (80)
- Basal cell carcinoma (76)
- beta-Alanine metabolism (74)
- Maturity onset diabetes of the young (72)
- Asthma (70)
- Linoleic acid metabolism (69)
- SNARE interactions in vesicular transport (68)
- Selenoamino acid metabolism (68)
- Ether lipid metabolism (66)
- Nicotinate and nicotinamide metabolism (66)
- Dorso-ventral axis formation (65)
- Glycine (65)
- serine and threonine metabolism (65)
- Mismatch repair (62)
- RNA polymerase (60)
- Phenylalanine metabolism (58)
- Glyoxylate and dicarboxylate metabolism (56)
- Ascorbate and aldarate metabolism (55)
- Pentose and glucuronate interconversions (55)
- Sulfur metabolism (55)
- Other glycan degradation (54)
- Riboflavin metabolism (54)
- Homologous recombination (52)
- Pantothenate and CoA biosynthesis (51)
- Renin-angiotensin system (49)
- Glycosaminoglycan degradation (48)
- Basal transcription factors (46)
- One carbon pool by folate (44)
- Taste transduction (43)
- alpha-Linolenic acid metabolism (42)
- Glycosphingolipid biosynthesis - lacto and neolacto series (41)
- Terpenoid backbone biosynthesis (41)
- Primary bile acid biosynthesis (40)
- C21-Steroid hormone metabolism (37)
- N-Glycan biosynthesis (37)
- O-Glycan biosynthesis (36)
- Limonene and pinene degradation (35)
- Folate biosynthesis (34)
- Glycosphingolipid biosynthesis - globo series (33)
- Thiamine metabolism (30)
- Biosynthesis of unsaturated fatty acids (28)
- Circadian rhythm - mammal (27)
- Heparan sulfate biosynthesis (27)
- Non-homologous end-joining (25)
- ABC transporters (24)
- Glycosylphosphatidylinositol(GPI)-anchor biosynthesis (23)
- Steroid biosynthesis (23)
- Keratan sulfate biosynthesis (22)
- Synthesis and degradation of ketone bodies (22)
- Chondroitin sulfate biosynthesis (20)
- Glycosphingolipid biosynthesis - ganglio series (20)
- leucine and isoleucine biosynthesis (20)
- Methane metabolism (19)
- Taurine and hypotaurine metabolism (18)
- Caffeine metabolism (17)
- Fatty acid elongation in mitochondria (17)
- Phenylalanine (16)
- Protein export (16)
- Ubiquinone and other terpenoid-quinone biosynthesis (16)
- tyrosine and tryptophan biosynthesis (16)
- Fatty acid biosynthesis (12)
- Lysine biosynthesis (12)
- Vitamin B6 metabolism (12)
- D-Glutamine and D-glutamate metabolism (11)
- Cyanoamino acid metabolism (10)
- Biotin metabolism (6)
- Lipoic acid metabolism (1)
