Native Proteins

Proteins

Native Proteins

Human

Bovine

Chicken

Mouse

GFAP

KRT18

KRT8

AHSG

NEFH

NEFM

10 mM Tris-HCl

2 mM dithiothreitol

the solution can be further dialyzed against PBS (phosphate buffered saline

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g. Dulbeccos PBS).
See References 2 and 3 for more details.

pH 7.4).
For Immunization purposes

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to Filaments is performed by dissolving in 6 M urea buffer (see above) at concentrations of approx. 0.5 mg/ml. Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g. Dulbeccos PBS).

pH 7.4).
For immunization purposes

ELISA.

Amyotrophic lateral sclerosis (ALS)

Pathogenic Escherichia coli infection

Spinal Cord

Liver

Serum

2

Yes