Iba1 (149-161) goat polyclonal antibody, Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
| Applications | ELISA, IHC, WB |
| Reactivities | Hamster, Human, Monkey, Mouse, Porcine, Rat, Bovine, Equine, Goat, Rabbit, Pig |






![Dot blot analysis of A42 and polyQ36 prefibrillaroligomers and fibrils. A42 and polyQ fibrils only stainwith OC serum (Cat.No AP31731PU-N), while A42 andpolyQ prefibrillar oligomers only react with A11 (Cat.No [AP31729PU-N]). Picture courtesy of Kayed et al.,](https://cdn.origene.com/catalog/product/assets/images/antibody/acris/ap31731pu-n-1-d.jpg?browse)

![Anti-Nestin Antibody - Western Blot. Western blot using Affinity Purified anti-Nestin antibody shows detection of a band ~220 kD corresponding to mouse Nestin (arrowhead). Approximately 30 g of MEF whole cell lysate was separated by SDS-PAGE using a 4-20% gradient gel. After transfer onto nitrocellulose, the membrane was blocked and then probed with the primary antibody diluted to 1:2000 overnight at 4°C. The membrane was then washed and reacted with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX ( for 45 min at room temperature. IRDye800 fluorescence image was captured using the Ddyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](https://cdn.origene.com/catalog/product/assets/images/antibody/acris/ap07829pu-n-1-w.jpg?browse)




![Immunofluorescence staining of human VEGFR-3/FLT-4 (red) in hFlt4-transfected MG63 cells. Monoclonal mouse anti-human FLT-4 clone 9D9 and polyclonal rabbit anti-human FLT-4 (D) [DP3511]: A and C are negative control with secondary antibody only; B (mAb) and D (pAb) with specific antibodies against human FLT-4. The experiment was performed by the research group of Dr. Wolfgang Holnthoner, Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Austrian Cluster for Tissue Regeneration, Donaueschingenstrasse 13, A-1200 Vienna, Austria.](https://cdn.origene.com/catalog/product/assets/images/antibody/acris/dm3512p-1-f.jpg?browse)

















![Immunoblotting: Osteopontin antibody was used at a 1/1,000 dilution to detect Osteopontin by Western blot. In lane 2 reactivity is shown against 250 ng of Human osteopontin. Lane 3 shows reactivity of MMP-cleaved osteopontin. Lane 1 shows the position of molecular weight markers. Use a 1/10,000 dilution of HRP conjugated Goat anti-Rabbit IgG ([R1454HRP]) for detection.](https://cdn.origene.com/catalog/product/assets/images/antibody/acris/r1565-1-w.jpg?browse)






