Antibodies

Human IgA alpha Antibody Peroxidase Conjugated

Applications WB: 1:1,000 - 1:5,000
IHC: 1:500 - 1:2,500
ELISA: 1:10,000 - 1:200,000
Reactivities Human
Conjugation HRP

Human IgG (H+L chain), adsorbed goat polyclonal antibody, HRP

Applications
ELISA: 1/200,000.
Western Blot: 1/1,000-1:5,000.
Immunohistochemistry: 1/500-1/2,500
Recommended Dilutions: This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:7,000 to 1:32,000 of the reconstitution concentration is suggested for this product.
Reactivities Human
Conjugation HRP

Human IgG (H+L chain) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/300,000
Western Blot: 1/5,000-1/25,000.
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Human
Conjugation HRP

Human IgG F(ab')2 Antibody Peroxidase Conjugated

Applications WB: 1:1,000 - 1:10,000
IHC: 1:500 - 1:2,500
ELISA: 1:10,000 - 1:50,000
Reactivities Human
Conjugation HRP

Human IgG (H&L) Antibody

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:20,000 - 1:100,000
Reactivities Human
Conjugation Unconjugated

Human IgM mouse monoclonal antibody, clone M15/8, FITC

Applications Flow cytometry.
Reactivities Human
Conjugation FITC

Human IgA + IgG + IgM goat polyclonal antibody, FITC

Applications Flow Cytometry: Use 10 µl of 0.1-0.5 µg/ml diluted antibody to label 106 cells in 100 µl.
Reactivities Human
Conjugation FITC

Human IgE mouse monoclonal antibody, clone IA2, HRP

Applications ELISA: 1/500 - 1/1000; This HRP conjugated antibody may be used as a detection reagent in a sandwich ELISA for IgE, utilising SM1814P as capture reagent.
Reactivities Human
Conjugation HRP

Human IgM mouse monoclonal antibody, clone R1/69, Supernatant

Applications Immunohistochemistry on Formalin-Fixed Paraffin Embedded Sections: Use a dilution of 1/25-1/50 in an ABC method (30 minutes at room temperature).
Enzymatic predigestion with proteolytic enzymes is recommended for paraffin embedded tissue sections.
Recommended Positive Control: Tonsil.
Reactivities Human
Conjugation Unconjugated

Human IgM rabbit polyclonal antibody, Purified

Applications Immunohistochemistry with normal and neoplastic tissues (formalin fixed paraffin-embedded): the antibody may be diluted 1:150-1:250 in an ABC method, incubation time 30 minutes.
Positive control: Tonsil.
Cellular localization: Cytoplasmic.
Reactivities Human
Conjugation Unconjugated

Human IgM rabbit polyclonal antibody, Purified

Applications Immunohistochemistry with normal and neoplastic tissues (formalin fixed paraffin-embedded): the antibody may be diluted 1:150-1:250 in an ABC method, incubation time 30 minutes.
Positive control: Tonsil.
Cellular localization: Cytoplasmic.
Reactivities Human
Conjugation Unconjugated

Human IgM (Mu chain, Fc-Region) (in vitro) mouse monoclonal antibody, clone 2B9

Reactivities Human
Conjugation Unconjugated

Human IgG (Fc heavy chain specific) mouse monoclonal antibody, clone EM-07, FITC

Applications Direct Immunofluorescence analysis: 2-5 µg/ml.
Reactivities Human
Conjugation FITC

Human IgE mouse monoclonal antibody, clone 4H10, FITC

Applications Flow cytometry: 2-5 µg/ml.
Reactivities Human
Conjugation FITC

Goat anti-rabbit IgG Antiserum, pretitered, polyclonal

Reactivities Human, Mammalian
Conjugation Unconjugated