Antibodies

Human IgG4 (pFC) mouse monoclonal antibody, clone HP6023 (also known as HO6023), Purified

Applications ELISA.
Western Blot.
Immunoblotting.
Immunohistochemistry on Frozen Sections.
Reactivities Human
Conjugation Unconjugated

Human IgA1 (alpha chain) mouse monoclonal antibody, clone B3506B4, Purified

Applications ELISA.
Western Blot.
Immunoblotting.
Immunohistochemistry on Frozen Sections.
Reactivities Human
Conjugation Unconjugated

Human IgA1 (alpha chain) mouse monoclonal antibody, clone B3506B4, AP

Applications Immunohistochemistry on frozen sections.
ELISA.
Western Blot.
Immunoblotting.
Reactivities Human
Conjugation AP

Human IgE mouse monoclonal antibody, clone 4H10, Purified

Applications Flow Cytometry: 4 µg/ml.
ELISA.
Western Blot.
Reactivities Human
Conjugation Unconjugated

Human IgG4 (pFC specific) mouse monoclonal antibody, clone NI 315 (HP 6206), Biotin

Applications To identify the presence of IgG4 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgG4, and Immunoblotting. As a second step an avidin or streptavidin conjugate of the customer’s choice have to be used.
General Recommended Dilutions:
Histochemical Use: 1/50-1/250.
ELISA: from 1/100 upwards.
Western blot: from 1/500 upwards.
Reactivities Human
Conjugation Biotin

F(ab')2 Human IgA (alpha chain) Antibody

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:20,000 - 1:100,000
Reactivities Human
Conjugation Unconjugated

Human IgA + IgG + IgM (adsorbed F(ab)2 Fragment) goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western: 1/1,000-1/10,000 or dot blot), ELISA (1/10,000-1/50,000), immunoperoxidase electron microscopy and Immunohistochemistry (1/500-1/2,500) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
This product has been assayed against 1.0 µg of Human IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/2,000 to 1/8,000 of the reconstitution concentration is suggested for this product.
Reactivities Human
Conjugation HRP

Human IgA + IgG + IgM goat polyclonal antibody, Biotin

Applications Western Blot: 1/5000-1/20000.
ELISA: 1/400000.
Immunohistochemistry on frozen and paraffin sections: 1/1000-1/5000.
Reactivities Human
Conjugation Biotin

Human IgM (Mu chain specific) goat polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other AP-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended dilutions
ELISA: 1/1,000-1/3,000.
Western blot: 1/500-1/2,000.
Immunohistochemistry: 1/200-1/1,000.
Reactivities Human
Conjugation AP

Human IgG (H&L) Antibody

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:20,000 - 1:100,000
Reactivities Human
Conjugation Unconjugated

Human IgG F(c) Antibody

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:20,000 - 1:100,000
Reactivities Human
Conjugation Unconjugated

Human (kappa chain) Antibody Peroxidase Conjugated

Applications WB: 1:1,000 - 1:10,000
IHC: 1:500 - 1:2,000
ELISA: 1:77,000
Reactivities Human
Conjugation HRP

Human IgG (H&L) Antibody

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:20,000 - 1:100,000
Reactivities Human
Conjugation Unconjugated

Human IgG (H&L) Antibody Alkaline Phosphatase Conjugated

Applications WB: 1:500-1:2,500
IHC: 1:200-1:1,000
ELISA: 1:2,000 - 1:10,000
Reactivities Human
Conjugation Alkaline Phosphatase

Human IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

Applications WB: 1:1,000 - 1:5,000
IHC: 1:500 - 1:2,500
ELISA: 1:10,000 - 1:100,000
Reactivities Human
Conjugation HRP