Antibodies

Human IgM mouse monoclonal antibody, clone R1/69, Supernatant

Applications Immunohistochemistry on Formalin-Fixed Paraffin Embedded Sections: Use a dilution of 1/25-1/50 in an ABC method (30 minutes at room temperature).
Enzymatic predigestion with proteolytic enzymes is recommended for paraffin embedded tissue sections.
Recommended Positive Control: Tonsil.
Reactivities Human
Conjugation Unconjugated

Human IgG (heavy chain) mouse monoclonal antibody, clone B33/20, Purified

Applications ELISA.
Reactivities Human
Conjugation Unconjugated

Human IgE mouse monoclonal antibody, clone I27/mAb27

Applications FACS surface, Western Blot, Basophil degranulation, Study of IgE binding to CD23 (Fc epsilon R2).
Usage recommendation
:
*This monoclonal antibody may be used between 1-10 μg/ml.
*Optimal dilution should be determined by each laboratory for each application.
*Coupled antibody: to maintain RT before use.
Reactivities Human
Conjugation Unconjugated

Human IgA (alpha chain) goat polyclonal antibody, Biotin

Applications Immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody
based assays using streptavidin or avidin conjugates:
ELISA: 1/60,000-1/600,000
Western Blot: 1/2,000-1/10,000
Immunohistochemistry: 1/1,000-1/5,000
Reactivities Human
Conjugation Biotin

Human IgA (alpha chain) goat polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/80,000.
Wester Blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Human
Conjugation Unconjugated

Human IgD (heavy chain) goat polyclonal antibody, FITC

Applications Flow Cytometry: Use 10 µl of Neat-1/10 diluted antibody to label 106 cells in 100 µl.
Immunocytochemistry: Neat-1/10.
Immunohistochemistry on Frozen Sections.
Reactivities Human
Conjugation FITC

Rat IgG2c rabbit polyclonal antibody, Purified

Applications ELISA: 1µg/ml - 10µg/ml.
Western Blot.
Flow Cytometry.
Reactivities Human, Rat
Conjugation Unconjugated

Human IgG (Fd region) mouse monoclonal antibody, clone HP6045, PE

Applications ELISA (both capture and detection).
Western Blot.
Radioimmunoassay
Reactivities Human
Conjugation PE

Human IgG (Fd region) mouse monoclonal antibody, clone HP6045, Biotin

Applications ELISA (both capture and detection).
Western Blot.
Radioimmunoassay
Reactivities Human
Conjugation Biotin

Human IgG (Fd region) mouse monoclonal antibody, clone HP6045, FITC

Applications ELISA (both capture and detection).
Western Blot.
Radioimmunoassay
Reactivities Human
Conjugation FITC

IGHG1 (heavy chain) mouse monoclonal antibody, clone ICO-97, Purified

Applications ELISA: Use Antibody without BSA for Coating.
Flow Cytometry: 0.5-1 μg/106 cells.
Immunohistochemistry on Frozen Sections: 0.5-1 µg/ml for 30 minutes at RT.
Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen.
Reactivities Human
Conjugation Unconjugated

IGHG1 (heavy chain) mouse monoclonal antibody, clone ICO-97, Purified

Applications ELISA: Use Antibody without BSA for Coating.
Flow Cytometry: 0.5-1 μg/106 cells.
Immunohistochemistry on Frozen Sections: 0.5-1 µg/ml for 30 minutes at RT.
Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen.
Reactivities Human
Conjugation Unconjugated