Antibodies

Mouse IgG (H+L chain), adsorbed donkey polyclonal antibody, HRP

Applications Suitable for Immunoblotting (1/2,000-1/20,000 for Western blot), ELISA (1/180,000), Immunoperoxidase electron microscopy and Immunohistochemistry (1/1,000-1/5,000) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/20,000 to 1/80,000 of the reconstitution concentration is suggested for this product.
Reactivities Mouse
Conjugation HRP

Mouse IgE (heavy chain) rat monoclonal antibody, clone LO-ME-3, HRP

Applications ELISA: 500ng/ml.
Reactivities Mouse
Conjugation HRP

ADA rabbit polyclonal antibody, HRP, Purified

Applications ELISA, IP, WB
Reactivities Bovine, Human, Mouse, Rat

Mouse IgG goat polyclonal antibody, HRP, Aff - Purified

Applications ELISA: 1/10000.
Western Blot: 1/5000-1/10000,
Immunohistochemistry.
Reactivities Mouse
Conjugation HRP

Mouse IgA (alpha chain specific) goat polyclonal antibody, HRP

Applications ELISA: 1/4,000-1/8,000.
Immunoblotting.
Immunohistochemistry.
Reactivities Mouse
Conjugation HRP

Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, HRP

Applications ELISA: 1/4,000-1/8,000.
Immunoblotting.
Immunohistochemistry.
Reactivities Mouse
Conjugation HRP

Mouse IgG (H+L chain) chicken polyclonal antibody, HRP

Applications ELISA: a 1/70,000 dilution gave an O.D. of 1.0 in a 15 minute reaction using Tetramethylbenzidine as substrate.
Western Blot: 1/200 -1/5000.
Immunocytochemistry.
Immunohistochemistry: 1/250 - 1/5000.
Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose gels and ELISA analysis.
Reactivities Mouse
Conjugation HRP

Mouse IgG3 goat polyclonal antibody, HRP

Applications

ELISA: 1/4000-1/8000.
Western Blot.
Immunohistochemistry on Frozen Sections.
Immunohistochemistry on Paraffin Sections.

Reactivities Mouse
Conjugation HRP

Mouse IgG2a goat polyclonal antibody, HRP

Applications ELISA: 1/4000-1/8000.
Western Blotting.
Immunohistochemistry on Frozen Sections.
Immunohistochemistry on Paraffin Sections.
Reactivities Mouse
Conjugation HRP

Mouse IgG1 goat polyclonal antibody, HRP

Applications ELISA: 1/4000-1/8000.
Western Blot.
Immunohistochemistry on Frozen Sections.
Immunohistochemistry onParaffin Sections.
Reactivities Mouse
Conjugation HRP

Mouse IgA goat polyclonal antibody, HRP

Applications Immunohistochemistry on frozen and paraffin sections.
ELISA: 1/4000 - 1/8000.
Western Blot.
Reactivities Mouse
Conjugation HRP

Mouse IgG2b goat polyclonal antibody, HRP

Applications ELISA: 1/4000-1/8000.
Western Blot.
Immunohistochemistry on Frozen Sections
Immunohistochemistry on Paraffin Sections.
Reactivities Mouse
Conjugation HRP

Mouse IgG1 (Ads. to Hu, Bov, Rb Serum Proteins) goat polyclonal antibody, HRP, Aff - Purified

Applications Mouse IgG1 Peroxidase Secondary antibody reagents are ideal for western blotting, Immunohistochemistry and ELISA as well as other antibody detection methods.
Recommended Dilutions:
ELISA: 1/20,000-1/100,000.
Western Blot: 1/2,000-1/20,000.
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Mouse
Conjugation HRP