Mouse IgA (alpha chain specific) goat polyclonal antibody, Biotin
| Applications | ELISA: 1/5,000-1/20,000. |
| Reactivities | Mouse |
| Conjugation | Biotin |
Antibodies
Mouse IgE (Fc specific) goat polyclonal antibody, HRP
| Applications | In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgE antibodies in mouse serum or other body fluids; in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemical and Cytochemical: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000. depending on the method used. |
| Reactivities | Mouse |
| Conjugation | HRP |
Human IgG (gamma chain specific) rabbit polyclonal antibody, Aff - Purified
| Applications | Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. Recommended Dilutions: ELISA: 1/20,000-1/100,000. Western Blot: 1/2,000-1/10,000. Immunohistochemistry: 1/1,000-1/5,000. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Mouse IgG (H+L chain) sheep polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA, immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/10,000-1/200,000 Western blot: 1/1,000-1/5,000. Immunohistochemistry: 1/500-1/2,500. |
| Reactivities | Mouse |
| Conjugation | HRP |
Guinea Pig IgM (Fc specific) goat polyclonal antibody, Biotin
| Applications | In immunocytochemical and immunohistochemical use for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using an reference antibody of goat origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in guinea pig serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions: Histochemical and Cytochemical: 1/100 - 1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/1000 - 1/5000. |
| Reactivities | Guinea Pig |
| Conjugation | Biotin |
Monkey IgG (Fab specific) rabbit polyclonal antibody, FITC
| Applications | Direct staining of fixed cell and tissue substrates; to demonstrate the intracellular presence of free or Ig-bound subunits of both kappa or lambda type. In general this conjugate is not recommended as direct or indirect screening reagent for If isotypes on surface membranes of vital lymphoid cells. The activity to the common Ig/Fab subunit may result in the staining of immunoglobulins bound to the Fc-receptors on non-lymphoid cells. Combinations of isotype-specific reagents should be used instead for this purpose. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80. |
| Reactivities | Monkey |
| Conjugation | FITC |
Mouse IgE (Fc specific) rabbit polyclonal antibody, Azide Free
| Applications | Can be used as unlabelled secondary antibody for indirect detection of IgE in Mouse cell, tissue substrates and body fluids in immunofluorescence and immunoenzyme assay methods; for the production of immunoconjugates with a selected marker; to prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier; as catching or detecting antibody reagent in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in Mouse serum or other body fluid. When applied in any Immunocytochemical or Histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established. Recommended Working Dilutions: Histochemical and Cytochemical Use: 1/100-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5000. Performance testing: Isotype specificity and quantitative specific recognition ability are further evaluated at the high level of sensitivity by direct singe and simultaneous double staining of different types of mouse cells, using counterstaining with reference conjugates with a different marker. The immunoconjugate is further tested in ELISA-type assays. |
| Reactivities | Mouse |
| Conjugation | Unconjugated |
Mouse IgG (H+L chain) rabbit polyclonal antibody, FITC
| Applications | Suitable for ELISA, Immunofluorescence Microscopy. Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. Recommended Dilutions: FLISA: 1/10,000-1/50,000. IF Microscopy: 1/1000-1/5000. |
| Reactivities | Mouse |
| Conjugation | FITC |
Cy3 Conjugated Goat Anti-Mouse IgG
| Applications | IF/ICC: 1:50-100; IHC: 1:50-100 |
| Reactivities | Mouse IgG |
| Conjugation | Cy3 |
Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, PE
| Applications | FLISA: ? 0.1 µg/ml Flow Cytometry: ? 0.1 µg/106 cells. |
| Reactivities | Mouse |
| Conjugation | PE |
Bovine IgG (H+L chain) rabbit polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (Western blot: 1/1,000-1/10,000 or Dot blot), ELISA (1/10,000-1/50,000), Immunoperoxidase electron microscopy and Immunohistochemistry (1/500-1/2,500) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: This product has been assayed against 1.0 µg of Bovine IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product. |
| Reactivities | Bovine |
| Conjugation | HRP |
Cy3 Conjugated Goat Anti-Rabbit IgG
| Applications | IF/ICC: 1:50-100; IHC: 1:50-100 |
| Reactivities | Rabbit IgG |
| Conjugation | Cy3 |
Rabbit IgA+IgG+IgM (H+L chain) goat polyclonal antibody, HRP
| Applications | ELISA (to identify and measure a specific IgG in rabbit serum or other body fluids). Immunocytochemistry. Immunohistochemistry. Useful in electron microscopy, since the complex between the conjugated antibody and the antigen has electron-dense properties. Dot blot. Western blot. General Recommended Dilutions: Histochemical and Cytochemical Use: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000. Working dilutions should be prepared by adding sterile PBS, pH 7.2, and should not be refrozen. |
| Reactivities | Rabbit |
| Conjugation | HRP |
Mouse IgD (Fc specific) goat polyclonal antibody, Azide Free
| Applications | Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble Immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase Immunochemistry. When applied in any Cytochemical or Histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Recommended Working Dilutions: Histochemical Use: 1/50-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5000. |
| Reactivities | Mouse |
| Conjugation | Unconjugated |
Rat IgA + IgG + IgM (H+L chain) goat polyclonal antibody, HRP
| Applications | ELISA: 1/100,000. Western blot: 1/500-1/3,000. Immunohistochemistry: 1/500-1/1,500. |
| Reactivities | Rat |
| Conjugation | HRP |
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Reactivities
- Mouse (396)
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