Secondary Antibodies

Human IgG (F(ab)2 specific) mouse monoclonal antibody, clone 4A11, Purified

Applications ELISA.
Western blotting.
Flow Cytometry.

Has been described for previous lots to work in Immunofluorescence.
Reactivities Human
Conjugation Unconjugated

Human IgG4 mouse monoclonal antibody, clone MRQ-44, Purified

Applications Immunohistochemistry on Frozen and Formalin-Fixed, Paraffin-Embedded Sections: 1/100-1/500. 
Preparation and Pretreatment:
1. Cut 3-4 μm section of formalin-fixed paraffin-embedded tissue and place on positively charged slides; dry overnight at 58°C.
2. Deparaffinize, rehydrate, and epitope retrieve; the preferred method is the use of Heat Induced Epitope Retrieval (HIER) techniques in conjunction with a pressure cooker. The preferred method allows for simultaneous deparaffinization, rehydration, and epitope retrieval. Upon completion, rinse with 5 changes of distilled or deionized water.
3. If using HRP detection system, place slides in peroxide block for 10 minutes; rinse. If using AP detection system, omit this step.
Positive Control: Tonsil.
Reactivities Human
Conjugation Unconjugated

Human IgA (secretory) mouse monoclonal antibody, clone SC-05, Purified

Applications Western blot.
Immunoprecipitation.
Immunohistochemistry on Paraffin Sections.
Immunohistochemistry on Frozen Sections.

Examples of positive Human tissues: Secretory mucosa of gastrointestinal and respiratory tract, epithelia of salivary glands, endometrium, endocervix, kidney, prostate, mammary gland.
Reactivities Human
Conjugation Unconjugated

Human IgE mouse monoclonal antibody, clone NI 307, FITC

Applications To identify the presence of IgE in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as direct and indirect immunofluorescence staining of cytoplasmic IgE and cell membrane IgE.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200.
Reactivities Human
Conjugation FITC

Human IgG (F(ab)2 specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, Aff - Purified

Applications Application(s): suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilution(s):
ELISA: 1:25,000
Western Blot: 1:1,000 - 1:5,000
Immunohistochemistry: 1:500 - 1:2,500
Reactivities Human
Conjugation Unconjugated

Human IgE goat polyclonal antibody, HRP

Applications ELISA: 1/10000-1/100000.
Reactivities Human, Monkey
Conjugation HRP

Human IgA (Secretory component) mouse monoclonal antibody, clone NI 194-4 (A89-039), Ascites

Applications To identify the presence of free or bound Secretory component in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, Indirect Immunoperoxidase and Immunofluorescence staining, Haemagglutination and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: from 1/50-1/200.
ELISA: form 1/500 upwards.
Western blot: from 1/1000 upwards.
Reactivities Human
Conjugation Unconjugated

Human IgA (Secretory component) mouse monoclonal antibody, clone NI 194-4 (A89-039), Biotin

Applications To identify the presence of free or bound Secretory component in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, Indirect Immunoperoxidase and Immunofluorescence staining, Haemagglutination and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: from 1/20 upwards
ELISA: form 1/25 upwards
Western blot: from 1/50 upwards.
Reactivities Human
Conjugation Biotin

Human IgE (Fc specific) goat polyclonal antibody, FITC

Applications Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, to identify and measure IgE in Human serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions: 1/20-1/80, depending on the method used.
Reactivities Human
Conjugation FITC

Human IgE mouse monoclonal antibody, clone 090-18132, Purified

Applications ELISA.
Recommended pairs for Sandwich Immunoassay:
Capture / Detection:
AM00919PU-N / AM00917PU-N.
AM00919PU-N / AM00918PU-N.
Reactivities Human
Conjugation Unconjugated

Human IgA + IgG + IgM goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/20,000-1/100,000.
Western blot: 1/2,000-1/10,000.
Immunoprecipitation: 1/1,000-1/5,000.
Reactivities Human
Conjugation HRP

Human IgG3 (Hinge Region) mouse monoclonal antibody, clone HP6050, HRP

Applications ELISA: 1/4,000-1/10,000.
Western blotting.
Dot- and Slot-immunoblotting.
Immunohistochemistry on Frozen Sections: 1/10-1/20.
Reactivities Human
Conjugation HRP

Human IgE mouse monoclonal antibody, clone BE5, PE

Applications Flow cytometry: 5 µg/ml.
Reactivities Human
Conjugation PE

Human IgE mouse monoclonal antibody, clone BE5, FITC

Applications Flow cytometry: 5 µg/ml.
Reactivities Human
Conjugation FITC