Secondary Antibodies

IgG (Immunglobulin G, light chain), mouse anti porcine, clone PHE425

Applications Tested for immunohistochemistry (IHC), ELISA and Western Blot, other applications not yet tested. Approximate working dilutions: IHC, frozen sections: 0.1µg/ml (1:2000) IHC, paraffin sections: 0.25µg/ml (1:800); Proteinase K pretreatment for antigen retrieval is recommended. Western Blot: 0.05 – 0.5µg/ml. ELISA: 20 - 50ng/ml (EC50 of 1ng/ml illustrated below) Optimal dilutions should be determined by the end user.
Reactivities Porcine
Conjugation Unconjugated

Swine IgG (H&L) Antibody

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:20,000 - 1:100,000
Reactivities Porcine
Conjugation Unconjugated

Porcine IgM mouse monoclonal antibody, clone 206-2, Aff - Purified

Applications ELISA: 0.1 µg/ml (1/4000).
Immunoprecipitation.
Immunohistochemistry on Frozen Sections: 0.2 µg/ml (1/2000).
Immunohistochemistry on Paraffin Sections (Does not react on routinely processed Paraffin Section).
Does not react in Western Blot with reduced and denatured samples.
Reactivities Porcine
Conjugation Unconjugated

Porcine IgG (H+L chain) rabbit polyclonal antibody, Azide Free

Applications ELISA. 
Dot blot.
Immunoblotting.
Indirect Immunofluorescence. 

Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
Recommended Working Dilutions:
Histochemistry Use: 1/50-1/250. 
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000.
Precipitin titre: 1/64 when tested against pooled normal Swine serum in agar-block immunodiffusion titration.
Reactivities Porcine
Conjugation Unconjugated

Porcine IgM (heavy chain) rabbit polyclonal antibody, Purified

Applications This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Recommended Dilutions:
FLISA: 1/10000-1/50000.
Immunofluorescence: 1/1000-1/5000.
Flow Cytometry: 1/500-1/2500.
Reactivities Porcine
Conjugation Unconjugated

Porcine IgG F(c) specific goat polyclonal antibody, Aff - Purified

Applications ELISA: 1/100-1/1000.
Western Blot: 1/1000-1/30,000.
Immunodiffusion.
Reactivities Porcine
Conjugation Unconjugated

Porcine IgA goat polyclonal antibody, Aff - Purified

Applications ELISA: 1/100-1/1000.
Immunodiffusion.
Reactivities Porcine
Conjugation Unconjugated

Porcine IgG (H+L chain) goat polyclonal antibody, Aff - Purified

Applications ELISA: 1:100 - 1:10,000.
Reactivities Porcine
Conjugation Unconjugated