Secondary Antibodies

Human IgD (Fc specific) goat polyclonal antibody, Azide Free

Applications This antiserum is intended to detect and identify IgD in serum or other body fluids using the Immunofixation technique. Like Immunoelectrophoresis Immunofixation is essentially a two step technique. Proteins in a complex mixture are separated by electrophoresis in a gel carrier, followed by immunoprecipitation in situ with the antiserum. Non-precipitated proteins are removed by washing and the precipitated complex is revealed with a protein stain which allows its exact localization.
Immunofixation may be the method of choice whenever a high level of sensitivity is required to identify a minor protein component against a high background of other proteins. It enables the detection and identification of more than one paraproteins in serum or of free light chain. The detection limit is approximately 0.5 to 1 mg/ml in the presence of normal levels of immunoglobulins.
Antibody titre: Precipitin titre not less than 1:16 when tested against an appropriate reference in agar immunodiffusion block titration.
Reactivities Human
Conjugation Unconjugated

Human IgG rabbit polyclonal antibody, Serum

Applications Immunoprecipitation.
Immunodiffusion.

For Ouchterlony:
Sample Size: 15 µl
Distance between wells: 5 mm
Test time: 4 days at 37°C
Antigens tested: Normal human serum, purified human IgG, normal mouse serum.
Results: Titre with NHS > 1:1000.
Reactivities Human
Conjugation Unconjugated

Human IgG rabbit polyclonal antibody, Ig Fraction

Applications Immunoprecipitation.
Immunodiffusion.
Reactivities Human
Conjugation Unconjugated

Human IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone 4E3, Purified

Applications ELISA: ≤ 5 µg/ml (Ref.1)
Immunofluorescence: ≤ 1 µg/10e6 cells.
Flow Cytometry.
Reactivities Human
Conjugation Unconjugated

Human IgE mouse monoclonal antibody, clone MH25/1, Purified

Applications Immunofluorescence.
Immunohistochemistry on frozen and paraffin sections: 1/5 – 1/10 (Based on the Indirect method, with a normal ABC method the dilution can be 5-10 times higher, but the optimal dilution should be tested by serial dilution.)
Dilute immediately before use with PBS or TRIS.
Localisation: D ε 1 epitope on Fc Fragment
Positive control: Polypus nose
Reactivities Human
Conjugation Unconjugated

Human IgM mouse monoclonal antibody, clone B572M, Purified

Applications ELISA.
Lateral flow assays.
Reactivities Human
Conjugation Unconjugated

Human IgG (Fc specific) mouse monoclonal antibody, clone MK1A6, Purified

Applications ELISA assay development of total IgG.
Passive hemagglutination assays.
Histological staining of Acetone Fixed Frozen tissue sections.
Reactivities Human
Conjugation Unconjugated

Human IgG (Fc CH2 Domain specific) mouse monoclonal antibody, clone 8A4, Purified

Applications ELISA.
Flow cytometry (1:50/1:100): use 10 µl of the suggested working dilution to label 10e6 cells in 100 µl.
Reactivities Human
Conjugation Unconjugated

Human IgE mouse monoclonal antibody, clone 090-11348, Purified

Applications ELISA.
Recommended antibody pair for Sandwich Immunoassay:
Capture / Detection
AM00919PU-N / AM00918PU-N
Reactivities Human
Conjugation Unconjugated

Human IgA (secretory) mouse monoclonal antibody, clone B781M, Purified

Applications ELISA: 1/20,000 dilution will yield > 1.0 O.D. using Alkaline Phosphatase conjugated Rabbit anti-Mouse Ig.
Reactivities Human
Conjugation Unconjugated

Human IgE mouse monoclonal antibody, clone 090-11368, Purified

Applications ELISA.
Recommended pair for Sandwich Immunoassay
Capture: AM00919PU-N
Detection: AM00917PU-N
Reactivities Human
Conjugation Unconjugated

Human IgD (heavy chain) goat polyclonal antibody, Purified

Applications ELISA.
Reactivities Human
Conjugation Unconjugated

Human IgM rat monoclonal antibody, clone HM-7, Aff - Purified

Applications ELISA.
Reactivities Human
Conjugation Unconjugated

Human IgM mouse monoclonal antibody, clone 47.2.E9, Purified

Applications ELISA (1/5000-1/10000)
Reactivities Human
Conjugation Unconjugated

Human IgA (Fc specific) mouse monoclonal antibody, clone 15D6, Purified

Applications ELISA: 1/500-1/1000.
Reactivities Human
Conjugation Unconjugated