Human IgM mouse monoclonal antibody, clone R1/69, Supernatant
| Applications | Immunohistochemistry on Formalin-Fixed Paraffin Embedded Sections: Use a dilution of 1/25-1/50 in an ABC method (30 minutes at room temperature). Enzymatic predigestion with proteolytic enzymes is recommended for paraffin embedded tissue sections. Recommended Positive Control: Tonsil. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgM (Mu chain, Fab-Region) mouse monoclonal antibody, clone MA2, Purified
| Applications | ELISA. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgG (heavy chain) mouse monoclonal antibody, clone B33/20, Purified
| Applications | ELISA. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Mouse IgG1 (heavy chain) rat monoclonal antibody, clone LO-MG1-2, Purified
| Applications | ELISA: 5 µg/ml as coating antibody. |
| Reactivities | Mouse |
| Conjugation | Unconjugated |
Mouse IgM (heavy chain) rat monoclonal antibody, clone LO-MM-3, Purified
| Applications | ELISA: 5 µg/ml (Coating). This antibody may be used as a Coating antibody in Sandwich ELISA in combination with LO-MM-9 (SM1025B) and Streptavidin-HRP as the detection reagents. |
| Reactivities | Mouse |
| Conjugation | Unconjugated |
Mouse IgG1 (heavy chain) rat monoclonal antibody, clone LO-MG1-13, Purified
| Applications | ELISA: 5 µg/ml as coating antibody. This antibody may be used as a Coating antibody in ELISA in combination with SM1017HRP as the detection reagent. |
| Reactivities | Mouse |
| Conjugation | Unconjugated |
Rat IgM (heavy chain) mouse monoclonal antibody, clone MARM-4, Purified
| Applications | ELISA: 5 µg/ml as Coating antibody in combination with clone MARK-1/MARL-15 (AM05512HR-N) as Detection reagent and purified IgM (SA025). |
| Reactivities | Rat |
| Conjugation | Unconjugated |
Human IgE mouse monoclonal antibody, clone I27/mAb27
| Applications | FACS surface, Western Blot, Basophil degranulation, Study of IgE binding to CD23 (Fc epsilon R2). Usage recommendation: *This monoclonal antibody may be used between 1-10 μg/ml. *Optimal dilution should be determined by each laboratory for each application. *Coupled antibody: to maintain RT before use. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgA (alpha chain) goat polyclonal antibody, Aff - Purified
| Applications | Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. Recommended Dilutions: ELISA: 1/80,000. Wester Blot: 1/2,000-1/10,000. Immunohistochemistry: 1/1,000-1/5,000. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Mouse IgG (Fc specific) goat polyclonal antibody, Ig Fraction
| Applications | ELISA: 1/20000-1/100000. Western Blot: 1/2000-1/10000. Immunohistochemistry: 1/1000-1/5,000. |
| Reactivities | Mouse |
| Conjugation | Unconjugated |
Chicken IgG / Chicken IgY mouse monoclonal antibody, clone 4G12, Purified
| Applications | Western Blot: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. ELISA: 0.1 µg/ml. Immunoprecipitation: 1 - 10 µg. |
| Reactivities | Chicken |
| Conjugation | Unconjugated |
Sheep IgM (H+L chain) rabbit polyclonal antibody, Azide Free
| Applications | ELISA. Dot blot. Immunoblotting. Indirect Immunofluorescence. Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Recommended Working Dilutions: Histochemistry Use: 1/50-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000. Precipitin titre: 1/64 when tested against pooled normal sheep serum in agar-block immunodiffusion titration. |
| Reactivities | Sheep |
| Conjugation | Unconjugated |
Sheep IgM (Fc specific) rabbit polyclonal antibody, Azide Free
| Applications | ELISA. Dot blot. Immunoblotting. Indirect Immunofluorescence. Can be used as unlabelled primary or secondary reagent for indirect detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to prepare conjugates of the user’s own choice; to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in nonisotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used. Recommended Working Dilutions: Histochemistry Use: 1/50-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000. Precipitin titre: 1/64 when tested against pooled normal sheep serum in agar-block immunodiffusion titration. |
| Reactivities | Sheep |
| Conjugation | Unconjugated |
Porcine IgG (H+L chain) rabbit polyclonal antibody, Azide Free
| Applications | ELISA. Dot blot. Immunoblotting. Indirect Immunofluorescence. Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Recommended Working Dilutions: Histochemistry Use: 1/50-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000. Precipitin titre: 1/64 when tested against pooled normal Swine serum in agar-block immunodiffusion titration. |
| Reactivities | Porcine |
| Conjugation | Unconjugated |
Sheep IgG (H+L chain) rabbit polyclonal antibody, Azide Free
| Applications | ELISA. Dot blot. Immunoblotting. Indirect Immunofluorescence. Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Recommended Working Dilutions: Histochemistry Use: 1/50-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5.000. Precipitin titre: 1/64 when tested against pooled normal sheep serum in agar-block immunodiffusion titration. |
| Reactivities | Sheep |
| Conjugation | Unconjugated |
