Secondary Antibodies

Goat Polyclonal Anti-Human IgG Antibody

Applications WB 1:500-1:2,000
Reactivities Human
Conjugation Unconjugated

Chicken IgM goat polyclonal antibody, Purified

Applications ELISA: (1/100-1/1000).
Immunodiffusion.
Reactivities Chicken
Conjugation Unconjugated

Equine IgM goat polyclonal antibody, Aff - Purified

Applications ELISA.
Immunodiffusion.
Reactivities Equine
Conjugation Unconjugated

Bovine IgG1 sheep polyclonal antibody, Aff - Purified

Applications ELISA.
Immunodiffusion.
Reactivities Bovine
Conjugation Unconjugated

Canine IgE mouse monoclonal antibody, clone E6-71A1, Purified

Applications ELISA.
Western Blotting: Under reducing conditions, this antibody detects a single major band of approximately 62-65 kDa in samples of affinity purified Dog IgE.
Reactivities Canine
Conjugation Unconjugated

Rat IgD (heavy chain) mouse monoclonal antibody, clone MARD-3, Purified

Applications Immunohistochemistry on Frozen Sections.
ELISA: This antibody may be used as a coating antibody in ELISA in combination with clone MARK-1/MARL-15 (Cat.-No AM05512HR-N) as detection reagent.
Reactivities Rat
Conjugation Unconjugated

Mouse IgG (H+L chain) goat polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/150,000.
Western Blot: 1/3,000-1/15,000.
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Mouse
Conjugation Unconjugated

Porcine IgM (heavy chain) rabbit polyclonal antibody, Purified

Applications This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Recommended Dilutions:
FLISA: 1/10000-1/50000.
Immunofluorescence: 1/1000-1/5000.
Flow Cytometry: 1/500-1/2500.
Reactivities Porcine
Conjugation Unconjugated

Turkey IgG (H+L chain) goat polyclonal antibody, Purified

Applications Immunofluorescence.
ELISA.
Western blotting.
Dot- and Slot-Immunoblotting.
Immunohistochemistry.
Reactivities Turkey
Conjugation Unconjugated

Mouse IgA (alpha chain specific) goat polyclonal antibody, Aff - Purified

Applications Immunoblotting.
Flow Cytometry.
Immunohistochemistry.
Enzyme-Linked-Immunosorbent-Assay (ELISA).
Fluorescent-Linked-Immunosorbent-Assay (FLISA).
Reactivities Mouse
Conjugation Unconjugated

Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, Aff - Purified

Applications

Indirect Immunostaining (In conjunction with mouse monoclonal antibodies).
Enzyme-Linked-Immunosorbent-Assay (ELISA)
Fluorescent-Linked-Immunosorbent-Assay (FLISA)
Immunoblotting.
Flow Cytometry.

Reactivities Mouse
Conjugation Unconjugated

Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, Aff - Purified

Applications ELISA: 1/10,000 dilutions produced a detectable signal, as measured using HRP-labeled Chicken anti-Goat IgG Antibody Cat.-No AP31796HR-N (1/5000 dilution).
Western blot (1/5000).
Immunocytochemistry.
Immunohistochemistry (1/250).
Immunoprecipitation (1/250).
Immunoelectrophoresis: Chicken serum (3 µl) was placed in the center well (at the clear circle) and then subjected to electrophoresis. After electrophoresis was complete, goat anti-chicken serum (75 μl) was placed in the lower trough and A30-106-10 (75 μl) was placed in the upper trough. After overnight incubation at 4°C, the gel was washed, fixed and stained with Coomassie. Note the single precipitin line between the center well and upper trough.
Reactivities Chicken
Conjugation Unconjugated

Human IgG (Fc specific) mouse monoclonal antibody, clone H2, Low Endotoxin

Applications Indirect Immunofluorescent staining of IgG+ human B lymphocytes.
ELISA.
Western blotting.
Dot- and Slot-Immunoblotting.
Immunohistochemistry on Frozen Sections.
Immunocytochemistry.
Reactivities Human
Conjugation Unconjugated

Human IgG (Fc specific) mouse monoclonal antibody, clone H2, Purified

Applications Indirect Immunofluorescent staining of IgG+ human B lymphocytes: ≤ 1 µg/106 cells.
ELISA.
Western blotting.
Dot- and Slot-Immunoblotting.
Immunohistochemistry on Frozen Sections.
Immunocytochemistry.
Reactivities Human
Conjugation Unconjugated

Human IgG2 (Fc specific) mouse monoclonal antibody, clone 31-7-4, Purified

Applications Fluorescent-linked-immunosorbent assay (FLISA)
ELISA.
Western blotting.
Dot- and Slot-Immunoblotting.
Immunohistochemistry (frozen Sections).
Immunocytochemistry.
Reactivities Human
Conjugation Unconjugated