Secondary Antibodies

Mouse IgG2a Antibody Rhodamine Conjugated Pre-adsorbed

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
IF: 1:1,000 - 1:5,000
FLISA: 1:20,000 - 1:100,000
Reactivities Mouse
Conjugation TRITC

Mouse IgG2b Antibody ATTO 425 Conjugated Pre-adsorbed

Applications WB: 1:4,000 - 1:20,000
IF: >1:5,000
FC: 1:500 - 1:2,500
FLISA: >1:20,000
Reactivities Mouse
Conjugation ATTO 425

Mouse IgM (H&L) Antibody Peroxidase Conjugated

Applications WB: 1:1,000 - 1:10,000
IHC: 1:500 - 1:2,500
ELISA: 1:10,000 - 1:50,000
Reactivities Mouse
Conjugation HRP

Mouse IgG (H+L chain), adsorbed goat polyclonal antibody, HRP

Applications ELISA: 1/500-1/1000.
Immunohistochemistry on Frozen Sections: 1/50.
Immunohistochemistry on Paraffin Sections.
Reactivities Mouse
Conjugation HRP

Mouse IgG (H+L chain) goat polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/150,000.
Western Blot: 1/3,000-1/15,000.
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Mouse
Conjugation Unconjugated

Mouse IgM (Fc specific) goat polyclonal antibody, TRITC

Applications Direct immunofluorescence staining of cytoplasmic Ig of appropriately treated cell and tissue substrates; to demonstrate immunoglobulins or specific antibodies in cells and tissues; to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions are usually between 1/10 and 1/80.
Reactivities Mouse
Conjugation TRITC

Mouse IgM (Fc specific) goat polyclonal antibody, Biotin

Applications Can be used in Immunocytochemical and Immunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases. To identify a specific antigen using a reference antibody of Mouse origin known to be of the IgM isotype in the middle layer of the indirect test procedure. In non-isotopic assay methodology (e.g. ELISA) to measure IgM in Mouse serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working Dilutions:
For histochemical and Cytochemical use are usually between 1/100 and 1/250
In ELISA and comparable non-precipitating antibody-binding assays between 1/1500 and 1/6000.
Reactivities Mouse
Conjugation Biotin

Mouse IgM (Fc specific) goat polyclonal antibody, FITC

Applications In direct staining of cytoplasmic IgM in fixed mouse cells and tissue substrates; to identify circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of mouse in the middle layer of the test procedure.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions are usually between 1/20 and 1/80.
Reactivities Mouse
Conjugation FITC

Mouse IgA (alpha chain specific) goat polyclonal antibody, PE

Applications FLISA.
Flow Cytometry.
Immunofluorescence:
≤ 0.1 µg/106 cells.
Immunohistochemistry.
Reactivities Mouse
Conjugation PE

Mouse IgA (alpha chain specific) goat polyclonal antibody, Aff - Purified

Applications Immunoblotting.
Flow Cytometry.
Immunohistochemistry.
Enzyme-Linked-Immunosorbent-Assay (ELISA).
Fluorescent-Linked-Immunosorbent-Assay (FLISA).
Reactivities Mouse
Conjugation Unconjugated

Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, Aff - Purified

Applications

Indirect Immunostaining (In conjunction with mouse monoclonal antibodies).
Enzyme-Linked-Immunosorbent-Assay (ELISA)
Fluorescent-Linked-Immunosorbent-Assay (FLISA)
Immunoblotting.
Flow Cytometry.

Reactivities Mouse
Conjugation Unconjugated

Mouse IgG3 Antibody Fluorescein Conjugated Pre-adsorbed

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
IF: 1:1,000 - 1:5,000
FC: 1:200 - 1:500
FLISA: 1:10,000 - 1:50,000
Reactivities Mouse
Conjugation FITC

Mouse IgG2b Antibody Fluorescein Conjugated Pre-adsorbed

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
IF: 1:1,000 - 1:5,000
FC: 1:200 - 1:500
FLISA: 1:10,000 - 1:50,000
Reactivities Mouse
Conjugation FITC

Mouse IgG3 Antibody Rhodamine Conjugated

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
IF: 1:1,000 - 1:5,000
ELISA: 1:2,000 - 1:10,000
FLISA: 1:20,000 - 1:100,000
Reactivities Mouse
Conjugation TRITC

Mouse IgG (Fc specific) goat polyclonal antibody, Biotin

Applications ELISA: 1/20,000-1/100,000
Western blot: 1/2,000-1/10,000
Immunohistochemistry: 1/1,000-1/5,000.
Note: This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:40,000 to 1:130,000 of the reconstitution concentration is suggested for this product.
Reactivities Mouse
Conjugation Biotin