Secondary Antibodies

Fab Rabbit IgG (H&L) Antibody Rhodamine Conjugated

Applications IF: 1:1,000 - 1:5,000
FC: 1:500 - 1:2,500
FLISA: 1:10,000 - 1:50,000
Reactivities Rabbit
Conjugation TRITC

Rabbit IgG (H+L chain) chicken polyclonal antibody, FITC

Applications Immunocytochemistry.
Immunohistochemistry (1/250).
Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose and for non-specific binding using Cryostat sections of unfixed embryonic chick tissues.
Reactivities Rabbit
Conjugation FITC

Rabbit IgG (H+L chain) chicken polyclonal antibody, HRP

Applications ELISA: a 1/20,000 dilution gave an O.D. of 1.0 in a 15 minute reaction using Tetramethylbenzidine as substrate.
Western Blot (1/5000).
Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose gels and ELISA analysis.
Reactivities Rabbit
Conjugation HRP

Rabbit IgG (H+L chain) (Fab Fragment) goat polyclonal antibody, FITC

Applications Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended dilutions:
Flow cytometry 1:2,000 - 1:10,000
Immunofluorescence 1:1,000 - 1:5,000
FLISA:1:10,000-1:50,000.
Reactivities Rabbit
Conjugation FITC

Rabbit IgG F(ab')2 specific goat polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, Conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
ELISA 1:30,000
Western Blot: 1/5,000
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Rabbit
Conjugation Unconjugated

Rabbit IgG (Fc specific) goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/110,000.
Western Blot: 1/2,000-1/20,000.
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Rabbit
Conjugation HRP

Rabbit IgG (H+L chain) goat polyclonal antibody, TRITC, Lyophilized

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000.
IF Microscopy: 1/1,000-1/5,000.
Reactivities Rabbit
Conjugation TRITC

F(ab')2 Rabbit IgG (H&L) Antibody

Applications WB: 1:2,000-1:10,000
IHC: 1:1,000-1:5,000
ELISA: 1:20,000 - 1:100,000
Reactivities Rabbit
Conjugation Unconjugated

Rabbit IgG F(c) Antibody

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:20,000 - 1:100,000
Reactivities Rabbit
Conjugation Unconjugated

Rabbit IgG (H+L chain) sheep polyclonal antibody, PE

Applications Flow Cytometry (Use 50 µl of 1/5-1/10 diluted antibody to label 10e6 cells in 100 µl).
Reactivities Rabbit
Conjugation PE

Rabbit IgA goat polyclonal antibody, Purified

Applications ELISA: 1/100 - 1/1000.
Immunodiffusion.
Reactivities Rabbit
Conjugation Unconjugated

Rabbit IgG (H+L chain) sheep polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000.
Flow Cytometry: 1/500-1/2,500.
IF Microscopy: 1/1,000-1/5,000.
Reactivities Rabbit
Conjugation TRITC

Rabbit IgG (H+L chain) F(ab)2 Fragment goat polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
FLISA: 1/1000 - 1/50,000
IF Microscopy: 1/1000 - 1/5000
Reactivities Rabbit
Conjugation FITC

Rabbit IgG (H+L chain) sheep polyclonal antibody, FITC

Applications Flow Cyometry: 1/25 - 1/50; Use 50µl of the suggested working dilution to label 10e6 cells in 100µl.
Immunohistochemistry on frozen sections: 1/25 - 1/50.
Reactivities Rabbit
Conjugation FITC

Rabbit IgA goat polyclonal antibody, HRP

Applications ELISA: 1/10000-1/100000.
Reactivities Rabbit
Conjugation HRP