Secondary Antibodies

Rabbit IgG (H+L chain) goat polyclonal antibody, AP, Liquid

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/3,000-1/25,000.
Western blot: 1/1,000-1/4,000.
Immunohistochemistry: 1/200-1/1,000.
Reactivities Rabbit
Conjugation AP

Biotin Conjugated Rabbit Anti-human IgG

Applications WB: 1:200-400; ICC: 1:100-200; IHC: 1:100-200; ELISA: 1:5,000-20,000
Reactivities Human IgG
Conjugation Biotin

Mouse IgG (H+L chain), adsorbed goat polyclonal antibody, FITC

Applications Flow Cytometry: Use 50µl of 1/50-1/200 diluted antibody to label 106 cells in 100 µl.
Reactivities Mouse
Conjugation FITC

Sheep IgG F(ab)2 specific rabbit polyclonal antibody, FITC

Applications This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. It is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
FLISA: 1/10,000-1/50,000.
IF Microscopy: 1/1,000-1/5,000.
Reactivities Sheep
Conjugation FITC

Human IgM (Mu chain specific) goat polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended dilutions:
ELISA: 1/10,000-1/45,000.
Western blot: 1/1,000-1/5,000.
Reactivities Human
Conjugation Unconjugated

Human IgM (Mu chain specific) goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions
ELISA: 1/80,000.
Western blot: 1/2,000-1/20,000.
Immunohistochemistry: 1/500-1/2,500.
Reactivities Human
Conjugation HRP

Monkey IgA (Secretory component) goat polyclonal antibody, Azide Free

Applications Tested in immunoelectrophoresis and double radial immunodiffusion against a panel of appropriate secretions and purified immunoglobulin isotypes.
As unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
Working dilutions:
Histochemistry: 1/50 - 1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/200 - 1/1000.
Reactivities Chimpanzee, Monkey
Conjugation Unconjugated

Monkey IgG (Fab specific) rabbit polyclonal antibody, Azide Free

Applications Can be used for indirect staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of free or Ig-bound subunits of both kappa and lambda type. In general this kind of products is not recommended as direct or indirect screening reagents for immunoglobulin isotypes on the surface of membranes of vital lymphoid cells. The presence of activity to the common Fab subunit may result in the staining of Ig bound to Fc-receptors on non-lymphoid cells. Combinations of isotype-specific reagents should be used instead for this purpose. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
Recommended Working Dilutions:
Histochemistry Use: 1/50-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000.
Reactivities Monkey
Conjugation Unconjugated

Rabbit IgG (H+L chain) goat polyclonal antibody, FITC, Lyophilized

Applications This product is designed for Immunofluorescence Microscopy, Fluorescence based plate assays (FLISA) and Fluorescent Western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000.
Immunofluoresence: 1/1,000-1/5,000.
Fluorescent Western Blot.
Reactivities Rabbit
Conjugation FITC

Mouse IgG1 goat polyclonal antibody, FITC

Applications Flow Cytometry: Use 10 µl of neat-1/10 diluted antibody to label 106 cells in 100 µl.
Immunohistochemistry on Frozen Sections.
Immunocytochemistry: neat-1/10.
Reactivities Mouse
Conjugation FITC

Mouse IgG (H+L chain), Fab Fragment goat polyclonal antibody, Aff - Purified

Applications Suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
For use in ELISA a dilution of 1:3,000-1:12,000 is recommended.
Reactivities Mouse
Conjugation Unconjugated

Guinea Pig IgG2 (subclass specific) goat polyclonal antibody, HRP

Applications This antibody can be used in Enzyme Immunocytochemical and Immunohistochemical staining for the detection of IgG2 at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates.
To demonstrate circulating IgG2 antibodies in serodiagnostic microbiology and autoimmune diseases. To identify a specific antigen using a reference antibody of guinea pig origin known to be of the IgG2 isotype in the middle layer of the indirect test procedure In non-isotopic assay methodology (e.g. ELISA) to measure IgG2 in guinea pig serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working Dilutions:
Histochemical and Cytochemical Use: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/5,0000.
Reactivities Guinea Pig
Conjugation HRP

Monkey IgA (Secretory component) goat polyclonal antibody, Biotin

Applications Tested in Immunoelectrophoresis, Double Radial Immunodiffusion and ELISA against a panel of appropriate secretions and purified Ig isotypes.
Can be used in Immunocytochemical and Immunohistochemical staining for the detection of free SC and secretory IgA at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. In non-isotopic assay methodology (e.g. ELISA) to identify and measure SC or secretory IgA in Monkey body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used.
Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working Dilutions:
Histochemical and Cytochemical Use: 1/50-1/200.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/1,000.
Reactivities Monkey
Conjugation Biotin

Mouse IgA (alpha chain specific) goat polyclonal antibody, FITC

Applications FLISA: 1/100–1/400.
Flow Cytometry: ≤ 1 μg/106 cells.
Immunofluorescence: ≤ 1 μg/106 cells.
Immunohistochemistry.
Reactivities Mouse
Conjugation FITC

Bovine IgG (H+L chain) goat polyclonal antibody, Agarose

Applications Suitable for antibody purification or to remove unwanted cross-reactivity.
Reactivities Bovine
Conjugation Agarose