Secondary Antibodies
Rabbit IgG (H+L chain, adsorbed) goat polyclonal antibody, Aff - Purified
| Applications | Suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user. ELISA: 1/15,000-1/30,000 Western blot: 1/500-1/2,000 Immunohistochemistry: 1/500-1/2,000. |
| Reactivities | Rabbit |
| Conjugation | Unconjugated |
Rabbit IgA+IgG+IgM (H+L chain) goat polyclonal antibody, Azide Free
| Applications | The cytochemical grade allows the use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates; in radioimmunoassay; for the production of immunoconjugates with a selected marker; to prepare immunoaffinity adsorbents by coupling to an artificial carrier; in non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent; in Western blotting. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used. Recommended Working Dilutions: Histochemical and Cytochemical Use: 1/100-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5000. |
| Reactivities | Rabbit |
| Conjugation | Unconjugated |
Rabbit IgA (Fc specific) goat polyclonal antibody, Azide Free
| Applications | As unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Recommended Working Dilutions: Histochemistry: 1/100-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/2500. Antibody titre: Precipitin titre not less than 1/32 when tested against pooled normal rabbit serum in agar-block immunodiffusion titration. |
| Reactivities | Rabbit |
| Conjugation | Unconjugated |
Rabbit IgA+IgG+IgM (H+L chain) goat polyclonal antibody, TRITC
| Applications | Can be used to identify and measure immunoglobulins, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates. If two immunoconjugates with different labels but originating from the same host animal species are used for direct two-color staining, they can safely be mixed and used in a single step, without the danger of interaction between the two reagents. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended working dilutions: 1/20 - 1/80. |
| Reactivities | Rabbit |
| Conjugation | TRITC |
Rabbit IgA+IgG+IgM (H+L chain) goat polyclonal antibody, FITC
| Applications | Can be used for direct immunofluorescence staining of cytoplasmic Ig of appropriately treated cell and tissue substrates; to demonstrate immunoglobulins or specific antibodies in cells and tissues; to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of rabbit origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended working dilutions: 1/20 - 1/80. |
| Reactivities | Rabbit |
| Conjugation | FITC |
Rabbit IgM (Fc specific) goat polyclonal antibody, FITC
| Applications | Can be used in Immunocytochemical and Immunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of rabbit origin known to be of the IgM isotype in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended working dilutions: 1/10 - 1/40. |
| Reactivities | Rabbit |
| Conjugation | FITC |
Rabbit IgM (Fc specific) goat polyclonal antibody, Azide Free
| Applications | Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Recommended working dilutions: Histochemistry: 1/50 - 1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500 - 1/3000. Antibody titre: Precipitin titre not less than 1/32 when tested against pooled normal rabbit serum in agar-block immunodiffusion titration. |
| Reactivities | Rabbit |
| Conjugation | Unconjugated |
Rabbit IgG (H+L chain) chicken polyclonal antibody, Aff - Purified
| Applications | ELISA. Western Blot (1/1000). Immunocytochemistry. Immunohistochemistry (1/250). Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose. |
| Reactivities | Rabbit |
| Conjugation | Unconjugated |
Rabbit IgG (H&L) Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed
| Applications | WB: 1:1,000 - 1:5,000 IHC: 1:400 - 1:2,000 ELISA: 1:10,000 - 1:20,000 |
| Reactivities | Rabbit |
| Conjugation | Alkaline Phosphatase |
Rabbit IgG (H&L) Antibody Peroxidase Conjugated
| Applications | WB: 1:1,000 - 1:3,000 IHC: 1:500 - 1:2,000 ELISA: 1:40,000 |
| Reactivities | Rabbit |
| Conjugation | HRP |
Rabbit IgG (H&L) Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed
| Applications | WB: 1:500 - 1:2,500 IHC: 1:200 - 1:1,000 ELISA: 1:50,000 - 1:100,000 |
| Reactivities | Rabbit |
| Conjugation | Alkaline Phosphatase |
Rabbit IgG (H&L) Antibody Biotin Conjugated Pre-Adsorbed
| Applications | WB: 1:2,000 - 1:10,000 IHC: 1:1,000 - 1:5,000 ELISA: 1:20,000 - 1:100,000 |
| Reactivities | Rabbit |
| Conjugation | Biotin |
Rabbit IgG (H&L) Antibody Pre-adsorbed
| Applications | WB: 1:3,000 - 1:30,000 IHC: 1:1,000 - 1:5,000 ELISA: 1:65,000 |
| Reactivities | Rabbit |
| Conjugation | Unconjugated |
Rabbit IgG (H&L) Antibody Pre-adsorbed
| Applications | WB: 1:2,000 - 1:10,000 IHC: 1:1,000 - 1:5,000 ELISA: 1:20,000 - 1:100,000 |
| Reactivities | Rabbit |
| Conjugation | Unconjugated |
