Human IgA rabbit polyclonal antibody, Purified
| Applications | Immunohistochemistry on Frozen and Paraffin Sections (ABC method: 1/25-1/100). Formalin-Fixed Paraffin Embedded Tissue Sections requires High temperature antigen unmasking with 10mM citrate buffer, pH 6.0 must be performed prior to the immunostaining. Incubation Time: 30 minutes at room temperature. Positive Control: Tonsil. |
| Reactivities | Human |
| Conjugation | Unconjugated |



![Western Blot of Goat Anti-Mouse IgG (H&L) Antibody. Lane M: Molecular Ladder. Lane 1: Mouse IgG whole molecule (p/n 010-0102). Lane 2: Mouse IgG F(c) Fragment (p/n 010-0103). Lane 3: Mouse IgG F(ab) Fragment (p/n 010-0105). Lane 4: Mouse IgM Kappa (p/n 010-0107). Lane 5: Mouse Serum (p/n [D308-05]). Load: 50ng per lane. Block: (p/n MB-070) for 30 min at RT. Primary Antibody: Anti-Mouse IgG (H&L) Antibody 1:1000 for 60 min at RT. Secondary antibody: HRP Donkey Anti-Goat IgG (p/n 705-703-125) 1:40,000 for 30 min at RT . Predicted/Observed Size: 28 and 55 kDa for Mouse IgG, F(c), F(ab), IgM Kappa, and Serum. Mouse F(c) migrates slightly higher.](https://cdn.origene.com/catalog/product/assets/images/antibody/secondary-antibody/115/610-1102-anti-mouse-igg-1-wb-4x3.jpg?browse)

![SDS PAGE Results of Goat Anti-Mouse IgG Antibody. Lane 1: Goat Anti-Mouse IgG Reduced [1.0µg]. Lane 2: Opal Prestained Molecular Weight Marker (p/n MB-210-0500).
Lane 3: Goat Anti-Mouse IgG Non-Reduced [1.0µg]. 4-20% Gel, Coomassie Stained.](https://cdn.origene.com/catalog/product/assets/images/antibody/secondary-antibody/115/210-1102-gt-a-mouseigg-1-sds-4x3.jpg?browse)
![SDS PAGE Results of Goat Anti-Bovine IgG Antibody. Lane 1: Goat Anti-Bovine IgG Reduced [10µg].
Lane 2: Opal Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 3: Goat Anti-Bovine IgG Non-Reduced [10µg].
4-20% Gel, Coomassie Stained.](https://cdn.origene.com/catalog/product/assets/images/antibody/secondary-antibody/115/201-1102-gt-a-bovine-igg-1-sds-4x3.jpg?browse)
![ELISA results of purified Goat Anti-Golden Syrian & Armenian Hamster IgG (Min X Mouse and Rat Serum Proteins) tested against purified Golden Syrian Hamster IgG and Armenian Hamster IgG. Each well was coated in duplicate with 1.0 µg of protein from different species. Golden Syrian Hamster IgG (Green Line), Armenian Hamster IgG (Red Line), Mouse IgG [p/n 010-0102] (Blue Line), and Rat IgG [012-0102] (Purple Line). The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer (p/n MB-060-1000), Donkey Anti-Goat IgG HRP conjugated (p/n 605-703-125), and TMB substrate (p/n TMBE-1000).](https://cdn.origene.com/catalog/product/assets/images/antibody/secondary-antibody/115/620-101-440-gsah-igg-1-e-4x3.jpg?browse)



![Dot Blot Results of F(ab')2 Donkey Anti-GOAT IgG [H&L] Antibody Min X Ch, GP, Ham, Hs, Hu, Ms, Rb, Rt Serum Proteins. Goat IgG (1) 100ng, (2) 33.33ng, (3) 11.11ng, (4) 3.70ng, (5) 1.23ng. Primary Antibody: F(ab')2 Dk-a-Gt IgG Mx8 at 1µg/mL for 1hr at RT. Secondary Antibody: Goat Anti-Donkey HRP at 1:40,000 for 30 mins at RT. Block: BlockOut Buffer (p/n MB-073) for 30 mins at RT. Exposure: 1 sec.](https://cdn.origene.com/catalog/product/assets/images/antibody/secondary-antibody/115/705-701-125-fab2-anti-goat-igg-1-db-4x3.jpg?browse)
![Western Blot of Anti-Rat IgG (H&L) (DONKEY) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins) (p/n 612-701-120). Lane M: 3 μl Molecular Ladder. Lane 1: Rat IgG whole molecule (p/n 012-0102). Lane 2: Rat IgG F(c) Fragment (p/n 012-0103). Lane 3: Rat IgG Fab Fragment (p/n 012-0105). Lane 4: Rat IgM Whole Molecule (p/n 012-0107). Lane 5: Rat Serum (p/n [D310-05]). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rat IgG (H&L) (DONKEY) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins) (p/n 612-701-120) 1:10,000 for 60 min at RT. Secondary Antibody: Anti-Donkey IgG (GOAT) Peroxidase Conjugated Antibody (p/n 616-1302) 1:40,000 in MB-070 for 30 min at RT. Predicted/Obsevered Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.](https://cdn.origene.com/catalog/product/assets/images/antibody/secondary-antibody/115/612-701-120-anti-rat-igg-donkey-mx10-antibody-1-wb-4x3.jpg?browse)


![ELISA Results of Donkey Anti-Sheep IgG Antibody (Min X Ch GP Ham Hs Hu Ms Rb Rt Serum Proteins) tested against purified Sheep IgG Mx8. Each well was coated in duplicate with 1.0 µg of Sheep IgG (p/n 013-0102) [Red Line], Chicken IgG (p/n 003-0102) [Green Line], Guinea Pig IgG (p/n 006-0102) [Blue Line], Hamster IgG (p/n 007-0102) [Purple Line], Horse IgG (p/n 008-0102), Human IgG (p/n 009-0102), Mouse IgG (p/n 010-0102), Rabbit IgG (p/n 011-0102), and Rat IgG (p/n 012-0102). The working dilution of Sheep IgG MX8 is 1:49,000. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer (p/n MB-076), Goat Anti-Donkey IgG HRP conjugated (p/n 616-4302), and TMB substrate (p/n TMBE-1000).](https://cdn.origene.com/catalog/product/assets/images/antibody/secondary-antibody/115/613-701-168-anti-sheep-igg-mx8-1-e-4x3.jpg?browse)