Secondary Antibodies

Bovine IgM sheep polyclonal antibody, HRP

Applications ELISA: 1/10,000 - 1/100,000.
Reactivities Bovine
Conjugation HRP

Chicken IgG / Chicken IgY (H+L chain) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
Western blot: 1/1,000-1/10,000.
ELISA: 1/10,000-1/50,000.
Immunohistochemistry: 1/500-1/2500.
Reactivities Chicken
Conjugation HRP

Human IgG (F(ab)2 specific) rabbit polyclonal antibody, HRP

Applications Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/10,000-1/50,000.
Western Blot: 1/1,000-1/10,000.
Immunohistochemistry: 1/500-2,500.
This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1/20,000 to 1/100,000 of the reconstitution concentration is suggested for this product.
Reactivities Human
Conjugation HRP

Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays.
Recommended Dilutions:
ELISA: 1/10,000-1/150,000.
Western blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/1,000-1/10,000.
Reactivities Chicken
Conjugation HRP

Hamster IgG goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. This product has been assayed against 1.0 µg of Hamster IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:15,000 to 1:65,000 of the reconstitution concentration is suggested for this product.
Reactivities Hamster
Conjugation HRP

Rat IgG2c goat polyclonal antibody, HRP

Applications ELISA: 1:10000.
Western Blot: 1:1000 - 1:10000.
Reactivities Rat
Conjugation HRP

Rat IgG1 goat polyclonal antibody, HRP

Applications ELISA: 1/10,000.
Western Blot: 1/1,000-1/10,000.
Reactivities Rat
Conjugation HRP

Mouse IgG (adsorbed) goat polyclonal antibody, HRP

Applications ELISA: 1/10000-1/100000.
Western Blot: 1/1000-1/10000.
Reactivities Mouse
Conjugation HRP

Rat IgM goat polyclonal antibody, HRP

Applications ELISA: 1:10,000 - 1:100,000.
Western Blot: 1:1000 - 1:10,000.
Reactivities Rat
Conjugation HRP

Rat IgA goat polyclonal antibody, HRP

Applications ELISA: 1:10,000.
Western Blot: 1:1,000 - 10,000.
Reactivities Rat
Conjugation HRP

Mouse IgG (Fc specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, HRP

Applications ELISA: 1/20000.
Western Blot: 1/500-1/2000.
Immunohistochemistry: 1/250-1/1000.
Reactivities Mouse
Conjugation HRP

Mouse IgM (Mu chain specific), F(ab)2 Fragment goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/100,000.
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/500-1/2,500.
Reactivities Mouse
Conjugation HRP

Rat IgG (H+L chain), Fab Fragment rabbit polyclonal antibody, HRP

Applications ELISA: 1/1000 - 1/2000.
Immunohistochemistry on frozen sections: 1/50.
Immunohistochemistry on paraffin sections: 1/50.
Immunohistochemistry on resin sections: 1/50.
Reactivities Rat
Conjugation HRP

Mouse IgG (H+L chain), F(ab)2 Fragment rabbit polyclonal antibody, HRP

Applications ELISA: 1/500 - 1/1000.
Western Blot: 1/2000 - 1/5000.
Immunohistochemistry on frozen, paraffin and resin sections: 1/50.
Reactivities Mouse
Conjugation HRP

Goat IgG (H+L chain), adsorbed rabbit polyclonal antibody, HRP

Applications Suitable for immunoblotting (Western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended dilutions
ELISA: 1/10,000-1/50,000.
Western blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/500-1/2,500.
Reactivities Goat
Conjugation HRP