Secondary Antibodies

Rabbit Ig (IgG + IgM) rat monoclonal antibody, clone SB87a, HRP

Applications ELISA: 1/4,000-1/8,000.
Reactivities Rabbit
Conjugation HRP

Mouse IgG1 (heavy chain) rat monoclonal antibody, clone LO-MG1-2, Purified

Applications ELISA: 5 µg/ml as coating antibody.
Reactivities Mouse
Conjugation Unconjugated

Mouse IgM (heavy chain) rat monoclonal antibody, clone LO-MM-3, Purified

Applications ELISA: 5 µg/ml (Coating).
This antibody may be used as a Coating antibody in Sandwich ELISA in combination with LO-MM-9 (SM1025B) and Streptavidin-HRP as the detection reagents.
Reactivities Mouse
Conjugation Unconjugated

Mouse IgG1 (heavy chain) rat monoclonal antibody, clone LO-MG1-13, Purified

Applications ELISA: 5 µg/ml as coating antibody.
This antibody may be used as a Coating antibody in ELISA in combination with SM1017HRP as the detection reagent.
Reactivities Mouse
Conjugation Unconjugated

Rat IgM (heavy chain) mouse monoclonal antibody, clone MARM-4, Purified

Applications ELISA: 5 µg/ml as Coating antibody in combination with clone MARK-1/MARL-15 (AM05512HR-N) as Detection reagent and purified IgM (SA025).
Reactivities Rat
Conjugation Unconjugated

Mouse IgE (heavy chain) rat monoclonal antibody, clone LO-ME-3, HRP

Applications ELISA: 500ng/ml.
Reactivities Mouse
Conjugation HRP

Human IgE mouse monoclonal antibody, clone I27/mAb27

Applications FACS surface, Western Blot, Basophil degranulation, Study of IgE binding to CD23 (Fc epsilon R2).
Usage recommendation
:
*This monoclonal antibody may be used between 1-10 μg/ml.
*Optimal dilution should be determined by each laboratory for each application.
*Coupled antibody: to maintain RT before use.
Reactivities Human
Conjugation Unconjugated

Chicken IgG / Chicken IgY mouse monoclonal antibody, clone 4G12, Purified

Applications Western Blot: 0.5 µg/ml for HRPO/ECL detection.
Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
ELISA: 0.1 µg/ml.
Immunoprecipitation: 1 - 10 µg.
Reactivities Chicken
Conjugation Unconjugated

Rabbit IgG (heavy chain) rat monoclonal antibody, clone RG-1, Aff - Purified

Applications ELISA.
Immunofluorescence.
Reactivities Rabbit
Conjugation Unconjugated

Rat IgG2a (gamma 2a chain specific) mouse monoclonal antibody, clone 2A 8F4, Biotin

Applications Flow Cytometry: ≤ 1 µg/106 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG2a+ cells.
ELISA: 1/5,000-1/20,000.
Reactivities Rat
Conjugation Biotin

Rat IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone G1 1C5, Purified

Applications Flow Cytometry.
Immunofluorescence Microscopy: Identification and enumeration of IgG1+ cells.
ELISA: Can be used as Capture antibody.
Second step reagent for rat IgG1 Monoclonal Antibodies.
Reactivities Rat
Conjugation Unconjugated

Chicken IgM (Mu heavy chain specific) mouse monoclonal antibody, clone M-4, Purified

Applications Flow Cytometry: ≤ 1 µg/106 cells.
Indirect Immunofluorescence staining. (Ref.1)
In ovo suppression of Chicken B lymphocytes.
In vitro activation of Chicken peripheral B lymphocytes.
Reactivities Chicken
Conjugation Unconjugated

Chicken IgA (alpha chain specific) mouse monoclonal antibody, clone A-1, Biotin

Applications ELISA.
Flow Cytometry: < / = 1 µg/10e6 cells.
Immunofluorescence.
Reactivities Chicken
Conjugation Biotin